LOCUS       V014931                 8241 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014931
VERSION     V014931
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    762..783
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        798..828
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    836..852
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     860..876
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        897..915
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        943..1169
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1170..1350
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1397..1522
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2015..2248
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2433..2477
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2626..2667
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        2775..3015
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    3086..3203
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        3252..3762
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3771..4478
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             4554..5576
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     primer_bind     complement(5603..5619)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5702..5935
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6007..6141
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6168..6303
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6324..6342)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6352..6368)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6510..6965
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6991..7095
                     /label="AmpR promoter"
     CDS             7096..7953
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8127..8241
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
       61 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      841 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
      901 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
      961 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1021 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1081 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1141 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1201 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1261 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1321 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1381 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1441 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1501 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1561 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1621 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1681 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1741 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1801 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1861 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1921 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1981 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2041 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2101 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2161 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2221 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2281 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2341 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2401 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     2461 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     2521 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2581 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2641 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2701 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg
     2761 ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat atacgataca
     2821 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     2881 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     2941 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     3001 tcttgtggaa aggacgaaac accggtccgc aggtatgcac gcgtgaattc tcgacctcga
     3061 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3121 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3181 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     3241 cgcggctcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     3301 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     3361 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     3421 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     3481 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     3541 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     3601 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     3661 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     3721 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc atggtgagca
     3781 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3841 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3901 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3961 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     4021 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4081 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     4141 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     4201 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     4261 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     4321 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     4381 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4441 gccgccactc caccggcggc atggacgagc tgtacaagga atttggcagc ggccagtgca
     4501 ccaactatgc cctgctgaaa ctggctggcg atgtggaaag caaccccgga cccatgaaaa
     4561 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4621 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4681 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4741 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4801 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4861 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     4921 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     4981 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5041 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5101 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5161 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5221 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5281 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5341 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5401 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5461 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5521 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     5581 gtagatgccg accgggatct atcgataccg tcgacctcga gggggggccc ggtaccttta
     5641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5701 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5941 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6001 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6121 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6181 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6241 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6301 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6481 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6841 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6961 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7021 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7081 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7141 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7201 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7261 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7321 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7381 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7441 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7501 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7561 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7621 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7681 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7741 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7801 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7861 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7921 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7981 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8041 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8101 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8161 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8221 actctttttc cgaaggtaac t
//
