LOCUS       V014014                 9027 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014014
VERSION     V014014
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             516..1307
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             1382..1615
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1687..1821
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1848..1983
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2004..2022)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2032..2048)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2190..2645
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2671..2775
                     /label="AmpR promoter"
     CDS             2776..3633
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3807..4395
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4683..4704
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4719..4749
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4757..4773
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4781..4797
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4818..4836
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4864..5090
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5091..5271
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5318..5443
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5936..6169
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6354..6398
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6547..6588
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        6696..6936
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    8858..8975
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9024..9027
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccatgat tgaacaagat ggattgcacg
      541 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      601 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      661 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      721 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      781 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      841 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      901 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      961 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     1021 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     1081 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1141 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1201 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1261 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggtaccttta
     1321 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     1381 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     1441 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1501 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1561 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     1621 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     1681 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1741 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1801 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     1861 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1921 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1981 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     2041 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     2101 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2161 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     2221 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2281 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2341 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2401 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2461 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2521 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2581 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2641 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3001 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3301 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3541 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3601 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4561 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4681 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     4741 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     4801 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     4861 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     4921 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     4981 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     5041 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     5101 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5161 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5221 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     5281 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     5341 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     5401 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     5461 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     5521 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     5581 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     5641 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     5701 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     5761 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     5821 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     5881 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     5941 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6001 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     6061 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     6121 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     6181 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     6241 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     6301 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     6361 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     6421 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     6481 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     6541 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     6601 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat
     6661 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt
     6721 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     6781 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     6841 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     6901 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggtgtg ttgtaaatga
     6961 gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa tcaaccaaaa
     7021 tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt tttgcaaact
     7081 tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt tgttttcttc
     7141 acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg cttcaatcat
     7201 tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag cattgtcttt
     7261 aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg aaaatcctgt
     7321 tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt tcatgatttc
     7381 ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc cagctgcttt
     7441 ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta atgcaccaat
     7501 atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa cttcgaatgc
     7561 ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta ttcctacatt
     7621 tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac aaactgttgc
     7681 aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg tagcatcttg
     7741 ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta tatattcatt
     7801 tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg aacagattca
     7861 ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg aacagcaaat
     7921 catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt atacattcca
     7981 tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa gggtgttgct
     8041 ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt aaaattcaag
     8101 tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa atagtctact
     8161 aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat agaagcttgt
     8221 attccatctt tatctctagc tgagtcatca attacatatc cataactttc ttcataagca
     8281 aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat tcatttaaat
     8341 ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct atcacccaaa
     8401 tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt taagcgtttt
     8461 agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc taatcttaca
     8521 aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc attcataata
     8581 ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc aggatcaaat
     8641 tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg ctcttcaacc
     8701 tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt tcctgttcca
     8761 ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt tttgtagaat
     8821 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     8881 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     8941 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9001 atccactttg gccgcggctc gaggggg
//
