LOCUS       V012881                 7104 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012881
VERSION     V012881
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    312..429
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        478..988
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1000..1716
                     /label="EGFP"
                     /note="enhanced GFP"
     LTR             1791..2024
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2096..2230
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2257..2392
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2413..2431)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2441..2457)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2599..3054
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3080..3184
                     /label="AmpR promoter"
     CDS             3185..4042
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4216..4804
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5092..5113
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5128..5158
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5166..5182
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5190..5206
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5227..5245
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5273..5499
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5500..5680
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5727..5852
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6345..6578
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6763..6807
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6956..6997
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gtccgcaggt atgcacgcgt gaattctcga cctcgagaca aatggcagta
      301 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      361 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      421 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg
      481 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
      541 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
      601 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
      661 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
      721 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
      781 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
      841 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
      901 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
      961 tgaccgaatc accgacctct ctccccaggg ggatccacca tggtgagcaa gggcgaggag
     1021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gccgggatca ctctcggcat ggacgagctg tacaagtaag gtacctttaa gaccaatgac
     1741 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     1801 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     1861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     1921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     1981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     2041 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     2101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2221 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2341 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     2401 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     2461 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2521 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2581 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2641 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2701 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2761 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2821 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2881 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2941 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3001 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3061 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4861 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4921 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4981 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5041 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5101 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5161 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5221 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     5281 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     5341 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     5401 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     5461 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     5521 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5581 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5641 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     5701 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     5761 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5821 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     5881 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     5941 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6001 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6061 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6121 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6181 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6241 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6301 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6361 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6421 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6481 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6541 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6601 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6661 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6721 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6781 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6841 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6901 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6961 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7021 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag
     7081 cctcgagcgg ccgccccctt cacc
//
