LOCUS       V013874                11100 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013874
VERSION     V013874
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     intron          516..1088
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        1143..1161
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1172..1792
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        1821..2320
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2341..2937
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    2954..3542
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3614..3847
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3919..4053
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4080..4215
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4236..4254)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4422..4877
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4903..5007
                     /label="AmpR promoter"
     CDS             5008..5865
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6039..6627
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6915..6936
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6951..6981
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        7050..7068
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7096..7322
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7323..7503
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7550..7675
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8168..8401
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8586..8630
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8779..8820
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        8919..9013
                     /label="H1-2O2 promoter"
                     /note="doxycycline-inducible variant of the human H1 RNA
                     promoter (Henriksen et al., 2007)"
     promoter        10000..10507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_feature    10931..11048
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt
      541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
      841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
      901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
      961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
     1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
     1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     1801 cggctcccga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     1861 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     1921 ttccacatcc tccggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     1981 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2041 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2101 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2161 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2221 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     2281 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     2341 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2401 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2461 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2521 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     2581 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2641 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2701 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     2761 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2821 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2881 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     2941 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     3361 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3541 gcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3601 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3841 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3901 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4021 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     4081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4141 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4201 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4261 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4321 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4381 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4861 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5221 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5401 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5521 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5641 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5881 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6181 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6781 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6841 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6901 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6961 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7021 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     7081 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     7141 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     7201 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7261 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7321 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7501 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7561 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7621 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7681 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7741 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7801 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7861 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7921 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7981 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     8041 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8101 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8161 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8221 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8281 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8341 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8401 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8461 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8521 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8581 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8641 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8701 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8761 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     8821 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     8881 gacggtatcg atcacgagac tagcctcgag cggccgcaat atttgcatgt cgctatgtgt
     8941 tctgggaaat caccataaac gtgaaatccc tatcagtgat agagacttat aagttcccta
     9001 tcagtgatag agacaccggt gtgttgtaaa tgagcacaca aaatacacat gctaaaatat
     9061 tatattctat gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa
     9121 taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa
     9181 acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact
     9241 tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac
     9301 tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc
     9361 ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc
     9421 ttcaacagct atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat
     9481 aatacattta acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac
     9541 taattcataa tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc
     9601 accatcttta attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag
     9661 aggaaaacct acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata
     9721 gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca
     9781 taattgttta atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt
     9841 gtttagtttc attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg
     9901 tctactttaa ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt
     9961 ttaggttcag ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt
    10021 agtaagtctt gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact
    10081 ttaagtccag caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt
    10141 tttaatttct tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc
    10201 atttctttaa ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat
    10261 caagcagcct ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca
    10321 tcaattacat atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct
    10381 tctttagcaa tttctctacc cattcattta aatccagtta aagtttttac aatattaact
    10441 ccatattttt catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga
    10501 gccggatttt ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa
    10561 attggtcctg tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt
    10621 ccaaatctgt cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca
    10681 tattcaagcg gtatttttca tgcaggatca aattctggat ttggatttac aacattttta
    10741 aatgtttcat cttcaaatgc atgctcttca acctcaataa cgttatatcc tgattcacgt
    10801 aatatttttg gggtaaattt agttcctgtt ccattaactg cgctaaaaat aatttttaaa
    10861 tcttttttag cttcttgctc ttttttgtag aattctcgac ctcgagacaa atggcagtat
    10921 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10981 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11041 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
//
