LOCUS       V015169                 8074 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015169
VERSION     V015169
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             379..1086
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1157..1274
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1323..1833
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1855..2451
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             2582..2815
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2887..3021
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3048..3183
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(3204..3222)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3232..3248)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3390..3845
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3871..3975
                     /label="AmpR promoter"
     CDS             3976..4833
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5007..5595
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5883..5904
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5919..5949
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5957..5973
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5981..5997
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6018..6036
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6064..6290
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    6518..6643
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7136..7369
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7554..7598
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7747..7788
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      421 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      481 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      541 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      601 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      661 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      721 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      781 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      841 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      901 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      961 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1021 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1081 tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctcgacctcg agacaaatgg
     1141 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1201 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1261 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     1321 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     1381 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     1441 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     1501 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     1561 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     1621 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     1681 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     1741 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     1801 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc
     1861 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1921 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1981 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     2041 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     2101 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2161 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2221 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2281 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2341 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2401 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     2461 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta
     2521 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     2581 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     2641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     2761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     2821 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     2881 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2941 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3001 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3061 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3121 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3181 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3361 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3421 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3481 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3541 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3601 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3661 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3721 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3781 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3841 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4201 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5821 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5881 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5941 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6001 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6061 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6121 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6181 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6241 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     6301 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6361 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6421 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6481 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6541 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6601 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6661 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6721 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6781 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6841 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6901 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6961 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7021 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7081 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7141 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7201 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7261 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7321 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7381 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7441 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7501 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7561 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7621 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7681 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7741 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     7801 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat
     7861 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt
     7921 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     7981 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     8041 cagttttaaa attatgtttt aaaatggact atca
//
