LOCUS       V015251                 8034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015251
VERSION     V015251
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             330..1046
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    1117..1234
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1283..1793
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1815..2411
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             2542..2775
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2847..2981
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3008..3143
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(3164..3182)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3192..3208)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3350..3805
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3831..3935
                     /label="AmpR promoter"
     CDS             3936..4793
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4967..5555
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5843..5864
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5879..5909
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5917..5933
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5941..5957
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5978..5996
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6024..6250
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6251..6431
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6478..6603
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7096..7329
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7514..7558
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7707..7748
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtag gtacattttg
      121 ttctagaaca aaatgtaccg gtacattttg ttctagatct gccgccccga ctgcatctgc
      181 gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat agaactaaag
      241 acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg ggccacgggg
      301 atgaagcgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      361 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      421 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      541 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      661 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      721 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      781 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      841 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      901 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      961 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1021 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
     1081 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1141 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1201 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1261 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     1321 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     1381 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     1441 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     1501 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     1561 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     1621 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     1681 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     1741 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     1801 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     1861 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     1921 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     1981 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2041 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2101 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2161 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2221 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2281 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2341 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2401 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     2461 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     2521 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2581 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2641 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2701 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2761 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2821 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     2881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2941 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3121 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3181 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3241 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3301 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     3361 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3421 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3481 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3541 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3601 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3661 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3721 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3781 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3901 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3961 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4021 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4081 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4141 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4201 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4261 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4321 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4381 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4441 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4501 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4561 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4621 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4681 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4741 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4801 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5101 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5341 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5581 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5641 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5701 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     5761 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5821 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     5881 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     5941 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6001 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6061 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6121 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6181 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     6241 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6301 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6361 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6421 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     6481 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     6541 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     6601 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     6661 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     6721 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     6781 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     6841 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     6901 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     6961 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7021 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7081 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7141 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7201 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7261 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     7321 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     7381 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     7441 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     7501 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     7561 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     7621 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     7681 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     7741 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     7801 cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg
     7861 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     7921 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     7981 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atca
//
