LOCUS       V013304                 7389 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013304
VERSION     V013304
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             375..398
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    472..589
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        638..1148
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1170..1766
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             1897..2130
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2202..2336
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2363..2498
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2519..2537)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2547..2563)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2705..3160
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3186..3290
                     /label="AmpR promoter"
     CDS             3291..4148
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4322..4910
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5198..5219
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5234..5264
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5272..5288
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5296..5312
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5333..5351
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5379..5605
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    5833..5958
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6451..6684
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6869..6913
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7062..7103
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga
      421 cgatatcttc gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
      481 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      541 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      601 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc
      661 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
      721 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
      781 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
      841 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
      901 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
      961 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     1021 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     1081 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     1141 ctccccaggg ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     1201 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     1261 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     1321 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     1381 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     1441 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     1501 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     1561 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     1621 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     1681 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1741 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     1801 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg
     1861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     1921 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     1981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2101 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     2161 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     2221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     2341 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2461 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     2521 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     2581 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2641 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2701 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2761 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2821 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2881 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2941 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3001 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3061 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3121 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5101 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5161 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5221 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5281 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     5341 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     5401 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     5461 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     5521 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     5581 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     5641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5761 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5821 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     5881 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     5941 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6001 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6061 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6121 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6181 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     6241 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     6301 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     6361 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6421 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     6481 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6541 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     6601 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     6661 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     6721 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     6781 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     6841 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     6901 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     6961 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7021 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7081 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     7141 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc
     7201 ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     7261 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     7321 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     7381 ggactatca
//
