LOCUS       V013185                 8002 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013185
VERSION     V013185
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        256..287
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             333..1040
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1085..1202
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1251..1761
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1783..2379
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             2510..2743
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2815..2949
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2976..3111
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(3132..3150)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3160..3176)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3318..3773
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3799..3903
                     /label="AmpR promoter"
     CDS             3904..4761
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4935..5523
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5811..5832
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5847..5877
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5885..5901
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5909..5925
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5946..5964
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5992..6218
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6219..6399
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6446..6571
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7064..7297
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7482..7526
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7675..7716
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtaa ggatgtccat
      121 attaggacat ctaggatgtc catattagga catctaggat gtccatatta ggacatctag
      181 gatgtccata ttaggacatc taggatgtcc atattaggac atctagatct ggcctcggcg
      241 gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg
      301 tactgttggt aaagccacca ccggtcgcca ccatggtgag caagggcgag gaggataaca
      361 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      421 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      481 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      541 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      601 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      661 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      721 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      781 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      841 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      901 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      961 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1021 gcatggacga gctgtacaag taggaattct cgacctcgag acaaatggca gtattcatcc
     1081 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     1141 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     1201 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     1261 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     1321 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     1381 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     1441 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     1501 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     1561 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     1621 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     1681 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     1741 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
     1801 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     1861 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     1921 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     1981 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     2041 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     2101 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     2161 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     2221 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     2281 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     2341 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     2401 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     2461 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2521 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     2581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     2761 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     2821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2941 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3121 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     3181 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3241 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3301 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3601 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3661 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3721 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     3781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3901 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3961 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4021 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4081 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4141 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4201 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4261 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4321 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4381 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4441 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4501 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4561 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4621 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4681 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4741 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4801 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4861 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4921 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4981 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5041 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5101 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5161 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5221 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5281 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5341 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5401 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5461 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5521 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5581 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5641 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5701 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     5761 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     5821 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     5881 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     5941 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6001 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6061 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6121 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     6181 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     6241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     6421 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     6481 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     6541 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     6601 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     6661 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     6721 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     6781 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     6841 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     6901 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     6961 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7021 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7081 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7141 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7201 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     7261 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7321 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7381 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     7441 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     7501 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     7561 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     7621 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     7681 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     7741 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     7801 ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
     7861 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     7921 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     7981 tatgttttaa aatggactat ca
//
