LOCUS       V014664                 8954 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014664
VERSION     V014664
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1073..1306
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1491..1535
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1684..1725
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1824..3035
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             3059..3871
                     /gene="TMEM192"
                     /label="Transmembrane protein 192"
                     /note="Transmembrane protein 192 from Homo sapiens.
                     Accession#: Q8IY95"
     CDS             3887..3913
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3926..3952
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3965..3991
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    4048..4165
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        4214..4724
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4746..5342
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             5473..5706
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5778..5912
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5939..6074
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6095..6113)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6123..6139)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6281..6736
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6762..6866
                     /label="AmpR promoter"
     CDS             6867..7724
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7898..8486
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8774..8795
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8810..8840
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8848..8864
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8872..8888
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8909..8927
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtaccat
     3061 ggcggcgggg ggcaggatgg aggacggttc cttggatatc acccagagta ttgaagacga
     3121 cccacttctg gatgcccagc ttctcccaca ccactcatta caagctcact ttagaccccg
     3181 attccatcct cttcctacag tcatcatagt gaatcttctg tggtttattc atctcgtgtt
     3241 tgttgtttta gcatttttaa caggtgtgct ttgttcttat cctaatccaa atgaggacaa
     3301 gtgcccagga aattacacaa acccattgaa agttcagacg gttataatcc ttgggaaagt
     3361 tattttgtgg attctccatt tactccttga atgctacatc cagtatcacc acagcaaaat
     3421 cagaaaccga ggctataact tgatctaccg atcaacaagg catctcaaga gacttgcgtt
     3481 gatgatacag tcctctggca acacagtgct tctcctcata ctgtgcatgc agcactcctt
     3541 cccagagcct ggcagattgt atcttgacct cattctggcc atcttggcac tggaactcat
     3601 ctgttccctg atatgtctcc tcatttacac agtgaaaatc cggagattta ataaagctaa
     3661 accagagcct gatatacttg aagaagaaaa aatctatgct taccccagca atattacctc
     3721 ggagactgga ttcagaacta tttcaagcct agaagaaatt gttgaaaagc aaggagacac
     3781 cattgaatac ctgaagcgac acaatgcgct gctgagtaag cgattgttgg ctctcacttc
     3841 ctcagacctg ggctgtcagc caagtagaac gggaggtgga ggtagttatc cctatgacgt
     3901 gcctgattac gccggcacag gatcctaccc ctatgatgtg cctgactacg ctggcagcgc
     3961 cggataccct tatgatgtgc ctgattatgc tggagggagc ggctaagaat tctcgacctc
     4021 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4081 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4141 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     4201 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     4261 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     4321 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     4381 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     4441 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     4501 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     4561 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     4621 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     4681 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggagc
     4741 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4801 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4861 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4921 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4981 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5041 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5101 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     5161 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5221 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5281 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5341 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     5401 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5461 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5521 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5701 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5761 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5881 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6001 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6061 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6721 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gtgcggaacc cctatttgtt
     6781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7081 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7141 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7201 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7261 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7321 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7381 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7441 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7501 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7561 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7621 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7681 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7861 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7921 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7981 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8041 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8101 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8161 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8221 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8281 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8341 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8401 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8461 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8521 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8581 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8641 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8701 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8761 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8821 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8881 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8941 agctgcaagc ttaa
//
