LOCUS       V014696                 8885 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014696
VERSION     V014696
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1073..1306
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1491..1535
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1684..1725
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1824..3035
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             3059..3871
                     /gene="TMEM192"
                     /label="Transmembrane protein 192"
                     /note="Transmembrane protein 192 from Homo sapiens.
                     Accession#: Q8IY95"
     CDS             3887..3934
                     /label="2xFLAG"
                     /note="two tandem FLAG(R) epitope tags"
     misc_feature    3979..4096
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        4145..4655
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4677..5273
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             5404..5637
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5709..5843
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5870..6005
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6026..6044)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6054..6070)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6212..6667
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6693..6797
                     /label="AmpR promoter"
     CDS             6798..7655
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7829..8417
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8705..8726
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8741..8771
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8779..8795
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8803..8819
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8840..8858
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtaccat
     3061 ggcggcgggg ggcaggatgg aggacggttc cttggatatc acccagagta ttgaagacga
     3121 cccacttctg gatgcccagc ttctcccaca ccactcatta caagctcact ttagaccccg
     3181 attccatcct cttcctacag tcatcatagt gaatcttctg tggtttattc atctcgtgtt
     3241 tgttgtttta gcatttttaa caggtgtgct ttgttcttat cctaatccaa atgaggacaa
     3301 gtgcccagga aattacacaa acccattgaa agttcagacg gttataatcc ttgggaaagt
     3361 tattttgtgg attctccatt tactccttga atgctacatc cagtatcacc acagcaaaat
     3421 cagaaaccga ggctataact tgatctaccg atcaacaagg catctcaaga gacttgcgtt
     3481 gatgatacag tcctctggca acacagtgct tctcctcata ctgtgcatgc agcactcctt
     3541 cccagagcct ggcagattgt atcttgacct cattctggcc atcttggcac tggaactcat
     3601 ctgttccctg atatgtctcc tcatttacac agtgaaaatc cggagattta ataaagctaa
     3661 accagagcct gatatacttg aagaagaaaa aatctatgct taccccagca atattacctc
     3721 ggagactgga ttcagaacta tttcaagcct agaagaaatt gttgaaaagc aaggagacac
     3781 cattgaatac ctgaagcgac acaatgcgct gctgagtaag cgattgttgg ctctcacttc
     3841 ctcagacctg ggctgtcagc caagtagaac gggaggtgga ggtagtgact acaaagacga
     3901 tgacgacaag gactacaaag acgatgacga caagtaagaa ttctcgacct cgagacaaat
     3961 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4021 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4081 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
     4141 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     4201 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     4261 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     4321 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     4381 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     4441 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     4501 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     4561 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     4621 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga
     4681 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4741 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4801 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4861 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4921 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4981 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5041 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5101 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5161 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5221 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     5281 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcggtacctt
     5341 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5401 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5461 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5521 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5581 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5641 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5701 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5821 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5941 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6001 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6061 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6121 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6181 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6421 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6541 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6661 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6901 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6961 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7021 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7081 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7141 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7201 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7261 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7321 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7381 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7441 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7501 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7561 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7621 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7681 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7741 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7801 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7861 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7921 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7981 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8041 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8101 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8161 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8221 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8281 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8341 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8401 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8461 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8521 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8581 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8641 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8701 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8761 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8821 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     8881 cttaa
//
