LOCUS       62056_14200        4992 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             933..2435
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     CDS             3457..4248
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4315..4903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaattaaga tctttttgat ggcagagttc agtttaccgg gtcacattgt acctgggaag
       61 attcaaggat ttatggaaaa agtcaacaac aggagtcaga gcagccggaa aagcatggac
      121 tctgtactta ggactgcgct ttgagcaatg gcacagcaag ctttaaccct gtttgcagtc
      181 agcacacaaa ctgtggttca aagctccact ttatctcttc ttgtggaatt cagatatcag
      241 atcagtttaa accttgcggc cgcactagtg ctcaaatggg agacaaagag attaagctct
      301 tatgtaaaat ttgctgtttt acataacttt aatgaatgga caaagtcttg tgcatggggg
      361 tgggggtggg gttagagggg aacagctcca gatggcaaac atacgcaagg gatttagtca
      421 aacaactttt tggcaaagat ggtatgattt tgtaatgggg taggaaccaa tgaaatgcga
      481 ggtaagtatg gttaataatc tacagttatt ggttaaagaa gtatattaga gcgagtcttt
      541 ctgcacacag atcaccttcc tatcaacccc actagcctct ggcaaaggta ccagtgtaca
      601 ggtttgtttc cttttttaaa atacattgag tatgcttgcc ttttagatat agaaatatct
      661 gatgctgtct tcttcactaa attttgatta catgatttga cagcaatatt gaagagtcta
      721 acagccagca cgcaggttgg taagtactgt gggaacatca cagattttgg ctccatgccc
      781 taaagagaaa ttggctttca gattatttgg attaaaaaca aagactttct taagagatgt
      841 aaaattttca tgatgttttc ttttttgcta aaactaaaga attattcttt tacatttcag
      901 tttttctgct agcaggcgcg ccagtcgaga ccatgctgct gctgctgctg ctgctgggcc
      961 tgaggctaca gctctccctg ggcatcatcc cagttgagga ggagaacccg gacttctgga
     1021 accgcgaggc agccgaggcc ctgggtgccg ccaagaagct gcagcctgca cagacagccg
     1081 ccaagaacct catcatcttc ctgggcgatg ggatgggggt gtctacggtg acagctgcca
     1141 ggatcctaaa agggcagaag aaggacaaac tggggcctga gatacccctg gccatggacc
     1201 gcttcccata tgtggctctg tccaagacat acaatgtaga caaacatgtg ccagacagtg
     1261 gagccacagc cacggcctac ctgtgcgggg tcaagggcaa cttccagacc attggcttga
     1321 gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc atctccgtga
     1381 tgaatcgggc caagaaagca gggaagtcag tgggagtggt aaccaccaca cgagtgcagc
     1441 acgcctcgcc agccggcacc tacgcccaca cggtgaaccg caactggtac tcggacgccg
     1501 acgtgcctgc ctcggcccgc caggaggggt gccaggacat cgctacgcag ctcatctcca
     1561 acatggacat tgacgtgatc ctaggtggag gccgaaagta catgtttcgc atgggaaccc
     1621 cagaccctga gtacccagat gactacagcc aaggtgggac caggctggac gggaagaatc
     1681 tggtgcagga atggctggcg aagcgccagg gtgcccggta tgtgtggaac cgcactgagc
     1741 tcatgcaggc ttccctggac ccgtctgtga cccatctcat gggtctcttt gagcctggag
     1801 acatgaaata cgagatccac cgagactcca cactggaccc ctccctgatg gagatgacag
     1861 aggctgccct gcgcctgctg agcaggaacc cccgcggctt cttcctcttc gtggagggtg
     1921 gtcgcatcga ccatggtcat catgaaagca gggcttaccg ggcactgact gagacgatca
     1981 tgttcgacga cgccattgag agggcgggcc agctcaccag cgaggaggac acgctgagcc
     2041 tcgtcactgc cgaccactcc cacgtcttct ccttcggagg ctaccccctg cgagggagct
     2101 ccatcttcgg gctggcccct ggcaaggccc gggacaggaa ggcctacacg gtcctcctat
     2161 acggaaacgg tccaggctat gtgctcaagg acggcgcccg gccggatgtt accgagagcg
     2221 agagcgggag ccccgagtat cggcagcagt cagcagtgcc cctggacgaa gagacccacg
     2281 caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc
     2341 aggagcagac cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg
     2401 cctgcgacct ggcgcccccc gccggcacca ccgacgccgc gcacccgggt tactctagag
     2461 tcggggcggc cggccgcttc gagcagacat gactcgactc ccgggatcca gagctcaccg
     2521 cggactcgag taacatcaca tttaaaagca tctcaggtaa ctatattttg aattttttaa
     2581 aaaagtaact gtaatagtta ttattaaaat agcaaagatt gaccatttcc aagagccata
     2641 tagaccagca ccgaccacta ttctaaacta tttatgtatg taaatattag cttttaaaat
     2701 tctcaaaata gttgctgagt tgggaaccac tattatttct atcgattcag cagccgtaag
     2761 tctaggacag gcttaaattg ttttcactgg tgtaaattgc agaaagatga tctaagtaat
     2821 ttggcattta ttttaatagg tttgaaaaac acatgccatt ttacaaataa gacttatatt
     2881 tgtccttttg tttttcagcc taccatgaga ataagagaaa gaaaatgaag atcaaaagct
     2941 tattcatctg tttttctttt tcgttggtgt aaagccaaca ccctgtctaa aaaacataaa
     3001 tttctttaat cattttgcct cttttctctg tgcttcaatt aataaaaaat ggaaagaatc
     3061 taatagagtg gtacagcact gttatttttc aaagatgtgt tgctatcctg aaaattctgt
     3121 aggttctgtg gaagttccag tgttctctct tattccactt cggtagagga tttctagttt
     3181 cttgtgggct aattaaataa atcattaata ctcttctaag ttatggatta taaacattca
     3241 aaataatatt ttgacattat gataattctg aataaaagaa caaaaaccat ggtataggta
     3301 aggaatataa aacatggctt ttaccttaga aaaaacaatt ctaaaattca tatggaatca
     3361 aaaaagagcc tgcaggcgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     3421 tgcaaatgac aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3601 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     3661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3841 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     3901 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     3961 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     4021 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4081 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4141 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4201 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     4261 tggtctagag ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac agatcgcggc
     4981 cgcaagagat ca
//
