LOCUS       62056_14195        6509 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             990..4034
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     CDS             4974..5765
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      5832..6420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaattaaga tctttttgat ggcagagttc agtttaccgg gtcacattgt acctgggaag
       61 attcaaggat ttatggaaaa agtcaacaac aggagtcaga gcagccggaa aagcatggac
      121 tctgtactta ggactgcgct ttgagcaatg gcacagcaag ctttaaccct gtttgcagtc
      181 agcacacaaa ctgtggttca aagctccact ttatctcttc ttgtggaatt cagatatcag
      241 atcagtttaa accttgcggc cgcactagtg ctcaaatggg agacaaagag attaagctct
      301 tatgtaaaat ttgctgtttt acataacttt aatgaatgga caaagtcttg tgcatggggg
      361 tgggggtggg gttagagggg aacagctcca gatggcaaac atacgcaagg gatttagtca
      421 aacaactttt tggcaaagat ggtatgattt tgtaatgggg taggaaccaa tgaaatgcga
      481 ggtaagtatg gttaataatc tacagttatt ggttaaagaa gtatattaga gcgagtcttt
      541 ctgcacacag atcaccttcc tatcaacccc actagcctct ggcaaaggta ccagtgtaca
      601 ggtttgtttc cttttttaaa atacattgag tatgcttgcc ttttagatat agaaatatct
      661 gatgctgtct tcttcactaa attttgatta catgatttga cagcaatatt gaagagtcta
      721 acagccagca cgcaggttgg taagtactgt gggaacatca cagattttgg ctccatgccc
      781 taaagagaaa ttggctttca gattatttgg attaaaaaca aagactttct taagagatgt
      841 aaaattttca tgatgttttc ttttttgcta aaactaaaga attattcttt tacatttcag
      901 tttttctgct agcaggcgcg ccagtcgact cccgggatcc actagtccag tgtggtggaa
      961 ttctgcagat cgaaacgatg atagatcccg tcgttttaca acgtcgtgac tgggaaaacc
     1021 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1081 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     1141 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
     1201 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
     1261 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
     1321 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
     1381 cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg
     1441 tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg
     1501 ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt tatctggaag
     1561 atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga
     1621 ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg
     1681 tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt
     1741 ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta
     1801 tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga
     1861 aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg
     1921 acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa
     1981 atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc
     2041 atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga
     2101 tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt
     2161 acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg
     2221 gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac
     2281 gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg
     2341 ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg
     2401 atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta
     2461 tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt
     2521 ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg
     2581 cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     2641 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     2701 atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     2761 gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg
     2821 aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga
     2881 ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg
     2941 atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt
     3001 tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac
     3061 gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc
     3121 agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc
     3181 atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta
     3241 accgccagtc aggctctctt tcacagatgt ggattggcga taaaaaccaa ctgctgacgc
     3301 cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga
     3361 cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg
     3421 aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg
     3481 ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg
     3541 atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc
     3601 cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg
     3661 gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg
     3721 atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct
     3781 gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca
     3841 tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg
     3901 aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct
     3961 ggagcccgtc agtatcggcg gaattccagc tgagcgccgg tcgctaccat taccagttgg
     4021 tctggtgtca aaaagcggcc gctcgagtaa catcacattt aaaagcatct caggtaacta
     4081 tattttgaat tttttaaaaa agtaactgta atagttatta ttaaaatagc aaagattgac
     4141 catttccaag agccatatag accagcaccg accactattc taaactattt atgtatgtaa
     4201 atattagctt ttaaaattct caaaatagtt gctgagttgg gaaccactat tatttctatc
     4261 gattcagcag ccgtaagtct aggacaggct taaattgttt tcactggtgt aaattgcaga
     4321 aagatgatct aagtaatttg gcatttattt taataggttt gaaaaacaca tgccatttta
     4381 caaataagac ttatatttgt ccttttgttt ttcagcctac catgagaata agagaaagaa
     4441 aatgaagatc aaaagcttat tcatctgttt ttctttttcg ttggtgtaaa gccaacaccc
     4501 tgtctaaaaa acataaattt ctttaatcat tttgcctctt ttctctgtgc ttcaattaat
     4561 aaaaaatgga aagaatctaa tagagtggta cagcactgtt atttttcaaa gatgtgttgc
     4621 tatcctgaaa attctgtagg ttctgtggaa gttccagtgt tctctcttat tccacttcgg
     4681 tagaggattt ctagtttctt gtgggctaat taaataaatc attaatactc ttctaagtta
     4741 tggattataa acattcaaaa taatattttg acattatgat aattctgaat aaaagaacaa
     4801 aaaccatggt ataggtaagg aatataaaac atggctttta ccttagaaaa aacaattcta
     4861 aaattcatat ggaatcaaaa aagagcctgc aggcggaatt gccagctggg gcgccctctg
     4921 gtaaggttgg gaagccctgc aaatgacaag agacaggatg aggatcgttt cgcatgattg
     4981 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5041 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5101 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5161 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5221 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5281 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5341 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5401 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5461 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5521 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5581 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5641 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5701 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5761 tcttctgagc gggactctgg tctagagttc cactgagcgt cagaccccgt agaaaagatc
     5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6481 gctcgacaga tcgcggccgc aagagatca
//
