LOCUS       40924_28297       11429 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLIC-C-TAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Popescu SC, Popescu GV, Bachan S, Zhang Z, Seay M, Gerstein M, 
            Snyder M, Dinesh-Kumar SP.
  TITLE     Differential binding of calmodulin-related proteins to their targets
            revealed through high-density Arabidopsis protein microarrays
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 104 (11), 4730-4735 (2007)
  PUBMED    17360592
REFERENCE   2  (bases 1 to 11429)
  AUTHORS   Popescu SC, Dinesh-Kumar SP.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2007) MCDB, Yale University, 219 Prospect Streeet,
            New Haven, CT 06520-8103, USA
REFERENCE   3  (bases 1 to 11429)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2007"; volume: "104"; issue: "11"; pages:
            "4730-4735"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2007) MCDB, Yale University, 219 Prospect Streeet, New 
            Haven, CT 06520-8103, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             737..1363
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1800..2864
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      2933..3127
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3471..3611
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3797..4385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4632..5420)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5948..5972
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6070..6244)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6282..6812)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6872..7550)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     7779..7795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7894..8567
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    8589..8644
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    8643..8667
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8831..8933
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             8964..9266
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(9320..9344)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9382..9411
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             9418..9447
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9454..9483
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9502..9531
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9538..9567
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9574..9603
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9622..9651
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9658..9687
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9694..9723
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9790..9807
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             9808..9831
                     /codon_start=1
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
                     /translation="LEVLFQGP"
     CDS             9871..10044
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             10048..10218
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     terminator      10291..10543
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10563..10579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10587..10603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10611..10641)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10656..10677)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    10842..10866
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
       61 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
      121 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
      181 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
      241 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
      301 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
      361 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
      421 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
      481 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
      541 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
      601 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
      661 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
      721 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
      781 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
      841 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
      901 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
      961 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1021 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1081 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     1141 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     1201 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     1261 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     1321 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     1381 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     1441 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     1501 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     1561 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     1621 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     1681 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     1741 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     1801 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     1861 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     1921 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     1981 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     2041 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     2101 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     2161 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     2221 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     2281 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     2341 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     2401 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     2461 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     2521 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     2581 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     2641 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     2701 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     2761 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     2821 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     2881 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     2941 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     3001 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     3061 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     3121 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     3181 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     3241 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     3301 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     3361 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3421 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     3481 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     3541 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3601 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4441 gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac
     4501 gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt
     4561 agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg tcggcttgaa cgaatttcta
     4621 gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     4681 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     4741 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     4801 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     4861 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     4921 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     4981 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     5041 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     5101 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     5161 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     5221 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     5281 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     5341 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     5401 gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     5461 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     5521 gatgcccgag gcatagactg taccccaaaa aaacatgtca taacaagaag ccatgaaaac
     5581 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgacgg
     5641 cagttacgct acttgcatta cagcttacga accgaacgag gcttatgtcc actgggttcg
     5701 tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     5761 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     5821 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     5881 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     5941 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6001 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctataggg
     6061 actttaggtg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     6121 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacgtga
     6181 gcgaaaccct ataagaaccc taattccctt atctgggaac tactcacaca ttattatgga
     6241 gaaactcgat gtcgatcgac tctagctaga acgaattgtt aggtggcggt acttgggtcg
     6301 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     6361 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     6421 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     6481 agatcataga tatagatctc actacgcggc tgctcaaacc tgggcagaac gtaagccgcg
     6541 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     6601 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     6661 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     6721 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     6781 tgctgcgtaa catcgttgct gctgcgtacc atggtcgatc gacagatctg cgaatcgaga
     6841 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
     6901 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     6961 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     7021 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     7081 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     7141 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     7201 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     7261 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
     7321 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     7381 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     7441 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     7501 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     7561 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     7621 tgttggcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
     7681 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     7741 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     7801 gcttgcatgc ctgcaggtca acatggtgga gcacgacact ctcgtctact ccaagaatat
     7861 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     7921 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     7981 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     8041 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     8101 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatggtc aacatggtgg
     8161 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
     8221 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     8281 ctatctgtca cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc
     8341 attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg
     8401 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     8461 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     8521 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggccg gtctagagta
     8581 tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa
     8641 ttacaagttt gtacaaaaaa gcaggcttcg acaagaaggc ctagatctcg actaagttgg
     8701 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag
     8761 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     8821 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     8881 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc
     8941 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     9001 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     9061 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     9121 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     9181 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     9241 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     9301 cataggcctc tcttcttcaa cccagctttc ttgtacaaag tggttgagct caagcttgcg
     9361 ggccgctcta gaactagtgg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
     9421 caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac
     9481 ttgaacggat cctctagagg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
     9541 caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac
     9601 ttgaacggat cctctagagg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
     9661 caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac
     9721 ttgaacggat ccactagtgg atcccccggg ctgcagccta gggattacga tatcccaacg
     9781 accgccagtc atcaccatca ccatcacctg gaagttctgt tccaggggcc cgagcttaag
     9841 acggccgccc tagcgcaaca cgatgaagcc gtagacaaca aattcaacaa agaacaacaa
     9901 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
     9961 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
    10021 ctaaatgatg ctcaggcgcc gaaagtagac aacaaattca acaaagaaca acaaaacgcg
    10081 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
    10141 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
    10201 gatgctcagg cgccgaaagt agacgcgaat tcgagctcgg tatgatccta gaggacgtct
    10261 cgaggtaccg cccgggcgag ctcggaannn gatcgttcaa acatttggca ataaagtttc
    10321 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
    10381 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
    10441 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
    10501 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcnnnngaa ttcgtaatca
    10561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10741 atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt gagcttggat
    10801 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
    10861 gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag
    10921 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
    10981 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
    11041 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
    11101 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
    11161 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
    11221 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
    11281 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
    11341 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
    11401 cccgcagcac ccgcgaccta ctggacatt
//
