LOCUS       V010549                 5505 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010549
VERSION     V010549
KEYWORDS    pLI50
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Lee CY, Buranen SL, Ye ZH
  TITLE     Construction of single-copy integration vectors for Staphylococcus
            aureus.
  JOURNAL   Gene. 1991 Jul 15. 103(1):101-5.
   PUBMED   1652539
REFERENCE   2  (bases 1 to 5505)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5505)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 1991
            Jul 15. 103(1):101-5."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(585..1232)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             1737..2738
                     /label="repB"
                     /note="RepB replication protein"
     primer_bind     complement(3247..3269)
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     misc_feature    3355..3495
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3510..3527)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3681..4269)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4443..5300)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5301..5405)
                     /label="AmpR promoter"
     primer_bind     5473..5491
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttctg
       61 taggttttta ggcataaaac tatatgattt acccctaaat ctttaaaatg ccccttaaaa
      121 ttcaaaataa aggcatttaa aatttaaata tttcttgtga taaagtttgt taaaaaggag
      181 tggttttatg actgttatgt ggttatcgat tataggtatg tggttttgta ttggaatggc
      241 attttttgct atcaaggtta ttaaaaataa aaattagacc acgcatttat gccgagaaaa
      301 tttattgtgc gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
      361 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt
      421 caataaagca attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat
      481 atggatctgg agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt
      541 agaaaaccga ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta
      601 ggcctatctg acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa
      661 acagaatgat gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat
      721 tttcctgctg taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac
      781 ccagtaaatg aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt
      841 ttgggaaaca atttccccga accattatat ttctctacat cagaaaggta taaatcataa
      901 aactctttga agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca
      961 tcaaaaattg tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg
     1021 taaccagttc taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca
     1081 gggtaaaatt tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct
     1141 gtggttatac taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc
     1201 caattgtcta aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa
     1261 agtgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct ttttttaaaa
     1321 gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat ccaattttcg
     1381 tttgttgaat ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     1441 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga
     1501 ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc
     1561 cttttctttc ttatcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa
     1621 ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt
     1681 gcacagaaaa accccatctg ttaaagttat aagtgactaa acaaataact aaatagatgg
     1741 gggtttcttt taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg
     1801 ttttagtgga caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta
     1861 atgttgatta ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag
     1921 attgtgctga aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc
     1981 gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac
     2041 atggcattca gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt
     2101 ggttgtttct cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt
     2161 tgtcagatat ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc
     2221 ttgttggttt tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc
     2281 agcacatgca tgtattggta tgtgtggaac caacttattt taagaataca gaaaactacg
     2341 tgaatcaaaa acaatggatt caattttgga aaaaggcaat gaaattagac tatgatccaa
     2401 atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa atcggatata caatcggcaa
     2461 ttgacgaaac tgcaaaatat cctgtaaagg atacggattt tatgaccgat gatgaagaaa
     2521 agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct
     2581 atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca gaagaaggcg
     2641 atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct attattgcaa
     2701 tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa cgggccagtt
     2761 tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt aggaagacga
     2821 gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga aaagcaaatc
     2881 taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg actagagaag
     2941 aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat ggggatgatg
     3001 ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc tattcggata
     3061 ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa tggacaaccg
     3121 gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt
     3181 atgcagtttg tagaatgcaa aaagtgaaat cagctgcctc gcgcgtttcg gtgatgacgg
     3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     3421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     4141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4741 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5041 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5401 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5461 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa
//
