LOCUS       V014120                 7472 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014120
VERSION     V014120
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             282..299
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             309..1271
                     /gene="mraY"
                     /label="Phospho-N-acetylmuramoyl-pentapeptide-transferase"
                     /note="Phospho-N-acetylmuramoyl-pentapeptide-transferase
                     from Staphylococcus aureus (strain JH9). Accession#:
                     A5IS68"
     CDS             1311..2027
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             complement(2603..3250)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3755..4756
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    5373..5513
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5699..6287)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6461..7318)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7319..7423)
                     /label="AmpR promoter"
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
       61 gaaaaatata cctgtatctt ttttcaaaag caaacatgct ttgggtaaac atgtaggtat
      121 taacgtcaat gcgacaatag tagcattgat taaatgagaa ttagtaagtg ttttacttac
      181 taaattttat ttaacctaaa aatgaaccac ctggatgtgt gggattaaaa agtgaagaga
      241 ggaggacata tcacttagga ggatgattat ttgagctcat gcatcatcat catcatcaca
      301 gcagcggcat gatttttgta tatgcgttat tagcgctagt gattacattt gttttggtac
      361 ctgttttaat acctacatta aaaaggatga aatttggtca aagtattcga gaagaaggtc
      421 cacaaagcca tatgaagaag actggtacac caacgatggg tggactaaca tttctattaa
      481 gtattgtgat aacgtctttg gtggctatta tatttgtaga tcaagctaat ccaatcatac
      541 tgttattatt tgtgacgatt ggttttgggt taattggttt tatagatgat tatattattg
      601 ttgttaaaaa gaataaccaa ggtttaacaa gtaaacagaa gtttttggcg caaattggta
      661 ttgcgattat attctttgtt ttaagtaatg tgtttcattt ggtgaatttt tctacgagca
      721 tacatattcc atttacgaat gtagcaatcc cactatcatt tgcatatgtt attttcattg
      781 ttttttggca agtaggtttt tctaatgcag taaatttaac agatggttta gatggattag
      841 caactggact gtcaattatc ggatttacaa tgtatgccat catgagcttt gtgttaggag
      901 aaacggcaat tggtattttc tgtatcatta tgttgtttgc acttttagga tttttaccat
      961 ataacattaa ccctgctaaa gtgtttatgg gagatacagg tagcttagct ttaggtggta
     1021 tatttgctac catttcaatc atgcttaatc aggaattatc attaattttt ataggtttag
     1081 tattcgtaat tgaaacatta tctgttatgt tacaagtcgc tagctttaaa ttgactggaa
     1141 agcgtatatt taaaatgagt ccgattcatc atcattttga attgatagga tggagcgaat
     1201 ggaaagtagt tacagtattt tgggctgttg gtctgatttc aggtttaatc ggtttatgga
     1261 ttggagtgca tggtacctct ggcggtggag gcagtggtgg cggaggctct atggtgagca
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ggtacccggg
     2041 gatcctctag agtcgacctg caggcatgca agcttctgta ggtttttagg cataaaacta
     2101 tatgatttac ccctaaatct ttaaaatgcc ccttaaaatt caaaataaag gcatttaaaa
     2161 tttaaatatt tcttgtgata aagtttgtta aaaaggagtg gttttatgac tgttatgtgg
     2221 ttatcgatta taggtatgtg gttttgtatt ggaatggcat tttttgctat caaggttatt
     2281 aaaaataaaa attagaccac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
     2341 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2401 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2461 aagcgaaaaa ggagaagtcg gttcagaaaa agaaggatat ggatctggag ctgtaatata
     2521 aaaaccttct tcaactaacg gggcaggtta gtgacattag aaaaccgact gtaaaaagta
     2581 cagtcggcat tatctcatat tataaaagcc agtcattagg cctatctgac aattcctgaa
     2641 tagagttcat aaacaatcct gcatgataac catcacaaac agaatgatgt acctgtaaag
     2701 atagcggtaa atatattgaa ttacctttat taatgaattt tcctgctgta ataatgggta
     2761 gaaggtaatt actattatta ttgatattta agttaaaccc agtaaatgaa gtccatggaa
     2821 taatagaaag agaaaaagca ttttcaggta taggtgtttt gggaaacaat ttccccgaac
     2881 cattatattt ctctacatca gaaaggtata aatcataaaa ctctttgaag tcattcttta
     2941 caggagtcca aataccagag aatgttttag atacaccatc aaaaattgta taaagtggct
     3001 ctaacttatc ccaataacct aactctccgt cgctattgta accagttcta aaagctgtat
     3061 ttgagtttat cacccttgtc actaagaaaa taaatgcagg gtaaaattta tatccttctt
     3121 gttttatgtt tcggtataaa acactaatat caatttctgt ggttatacta aaagtcgttt
     3181 gttggttcaa ataatgatta aatatctctt ttctcttcca attgtctaaa tcaattttat
     3241 taaagttcat ttgatatgcc tcctaaattt ttatctaaag tgaatttagg aggcttactt
     3301 gtctgctttc ttcattagaa tcaatccttt ttttaaaagt caatattact gtaacataaa
     3361 tatatatttt aaaaatatcc cactttatcc aattttcgtt tgttgaattt ttttaaaagt
     3421 caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc aattttcgtt
     3481 tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa atgtggtctt
     3541 ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt atcttgatac
     3601 atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg
     3661 tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt
     3721 aaagttataa gtgactaaac aaataactaa atagatgggg gtttctttta atattatgtg
     3781 tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag
     3841 tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact ttgaacttct
     3901 gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta
     3961 taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag
     4021 gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt
     4081 tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt
     4141 taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt
     4201 tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac
     4261 ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg tattggtatg
     4321 tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca
     4381 attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg
     4441 accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc
     4501 tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga
     4561 tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat
     4621 acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga
     4681 cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa
     4741 ttattttatt aaagagtagt tcaacaaacg ggccagtttg ttgaagatta gatgctataa
     4801 ttgttattaa aaggattgaa ggatgcttag gaagacgagt tattaatagc tgaataagaa
     4861 cggtgctctc caaatattct tatttagaaa agcaaatcta aaattatctg aaaagggaat
     4921 gagaatagtg aatggaccaa taataatgac tagagaagaa agaatgaaga ttgttcatga
     4981 aattaaggaa cgaatattgg ataaatatgg ggatgatgtt aaggctattg gtgtttatgg
     5041 ctctcttggt cgtcagactg atgggcccta ttcggatatt gagatgatgt gtgtcatgtc
     5101 aacagaggaa gcagagttca gccatgaatg gacaaccgga aagagcgaaa atgcctcaca
     5161 tttgtgccac ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa
     5221 agtgaaatca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     5401 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     5461 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     5521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6121 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     7081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7441 tgccacctga cgtctaagaa accattatta tc
//
