LOCUS       V015128                 8511 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015128
VERSION     V015128
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             282..299
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             309..1271
                     /gene="mraY"
                     /label="Phospho-N-acetylmuramoyl-pentapeptide-transferase"
                     /note="Phospho-N-acetylmuramoyl-pentapeptide-transferase
                     from Staphylococcus aureus (strain JH9). Accession#:
                     A5IS68"
     CDS             1311..2027
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             complement(2654..4030)
                     /gene="tet"
                     /label="Tetracycline resistance protein"
                     /note="Tetracycline resistance protein from Staphylococcus
                     aureus. Accession#: P02983"
     CDS             4794..5795
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    6412..6552
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6738..7326)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7500..8357)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8358..8462)
                     /label="AmpR promoter"
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
       61 gaaaaatata cctgtatctt ttttcaaaag caaacatgct ttgggtaaac atgtaggtat
      121 taacgtcaat gcgacaatag tagcattgat taaatgagaa ttagtaagtg ttttacttac
      181 taaattttat ttaacctaaa aatgaaccac ctggatgtgt gggattaaaa agtgaagaga
      241 ggaggacata tcacttagga ggatgattat ttgagctcat gcatcatcat catcatcaca
      301 gcagcggcat gatttttgta tatgcgttat tagcgctagt gattacattt gttttggtac
      361 ctgttttaat acctacatta aaaaggatga aatttggtca aagtattcga gaagaaggtc
      421 cacaaagcca tatgaagaag actggtacac caacgatggg tggactaaca tttctattaa
      481 gtattgtgat aacgtctttg gtggctatta tatttgtaga tcaagctaat ccaatcatac
      541 tgttattatt tgtgacgatt ggttttgggt taattggttt tatagatgat tatattattg
      601 ttgttaaaaa gaataaccaa ggtttaacaa gtaaacagaa gtttttggcg caaattggta
      661 ttgcgattat attctttgtt ttaagtaatg tgtttcattt ggtgaatttt tctacgagca
      721 tacatattcc atttacgaat gtagcaatcc cactatcatt tgcatatgtt attttcattg
      781 ttttttggca agtaggtttt tctaatgcag taaatttaac agatggttta gatggattag
      841 caactggact gtcaattatc ggatttacaa tgtatgccat catgagcttt gtgttaggag
      901 aaacggcaat tggtattttc tgtatcatta tgttgtttgc acttttagga tttttaccat
      961 ataacattaa ccctgctaaa gtgtttatgg gagatacagg tagcttagct ttaggtggta
     1021 tatttgctac catttcaatc atgcttaatc aggaattatc attaattttt ataggtttag
     1081 tattcgtaat tgaaacatta tctgttatgt tacaagtcgc tagctttaaa ttgactggaa
     1141 agcgtatatt taaaatgagt ccgattcatc atcattttga attgatagga tggagcgaat
     1201 ggaaagtagt tacagtattt tgggctgttg gtctgatttc aggtttaatc ggtttatgga
     1261 ttggagtgca tggtacctct ggcggtggag gcagtggtgg cggaggctct atggtgagca
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ggtacccggg
     2041 gatcctctag agtcgacctg caggcatgca agcttctgta ggtttttagg cataaaacta
     2101 tatgatttac ccctaaatct ttaaaatgcc ccttaaaatt caaaataaag gcatttaaaa
     2161 tttaaatatt tcttgtgata aagtttgtta aaaaggagtg gttttatgac tgttatgtgg
     2221 ttatcgatta taggtatgtg gttttgtatt ggaatggcat tttttgctat caaggttatt
     2281 aaaaataaaa attagaccac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
     2341 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2401 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2461 aagcgaaaaa ggagaagtcg gttcagaaaa agaaggatat ggatctggag ctgtaatata
     2521 aaaaccttct tcaactaacg gggcaggtta gtgacattag aaaaccgact gtaaaaagta
     2581 cagtcggcat tatctcatat gagtggcaaa atgctagcca ctcatagttc taaaccaaaa
     2641 tataatataa ctattcaaac tgcttttcag aacgtttaaa tacaataatc gtcaaaagac
     2701 aacataaaat aataaggata gccatggcta caagaatatt actatacact ccagaagaat
     2761 aatttataaa ttccagaact agtttacgat taatcaattg tagtgacaat aaacctccta
     2821 caattgctat acctgttccc tctgataaaa aacttgtgaa atttagcaaa ctcattccag
     2881 aagcaacttc ttcttcagaa agactacttg atactatttt tgatataact gttttagtaa
     2941 aagataatcc gcccataaca aatataaaca taaaagtagt caaccacata ctaaactcaa
     3001 caaaaaatgc aatagttaaa aaacttatag agatagacaa tgatcctaaa ataaaaacaa
     3061 ataatgatcc ttttctatcc actaaaaaac caccaaaata accaaaaaca ataacactca
     3121 tggttccagg aaaaataaca ctattaccta ttgtcgctac atttacatga taaatagttt
     3181 tcatcatata aggcaccatt gatataaaac cagctactat agaaaatatt agcccaccag
     3241 aaaacaaacc aagcataaac ggaatgtttt tccctagttt aggattaata aaagggttag
     3301 aaactcttga aatatgttta ataaaaatca caaaaaagat tgtgaagagt attaaaaaag
     3361 tccaattata atttgtcgta aataacataa aacatataat acttatagac attaaaacaa
     3421 tacctacgat atctaatgta ttttttgttg atttaccagg taccattact ttaataagaa
     3481 aaggtatagt tactattgta atcataggaa gtataagtag gtaagaccaa tgaatataat
     3541 gtgctattat tccccctatt gaaggaccta acccttcacc taaagctaca attgatccta
     3601 taaaaccaaa ggctttgcct tgtttttttc ttgtaatatt tctagctaca accaccataa
     3661 tcagtgaagg gaatgcagca gatcctactc cttgtactaa cctaccaaaa atcaaaataa
     3721 aaaagtgatt gtgaccaata aaagcaatca atgaaccaag acagctcaaa ctaataccaa
     3781 taattaacaa tttttttata tttatataat cagataattt tccatatact gctgttccta
     3841 tcgaaaaagt taacatatat gcagtgttta cccagtttgt aattccagga gtagtattaa
     3901 aatgatttgc aatatcaggt aaagaaacat ttaaaaccat ttcatttaat acactaaaaa
     3961 atgaaagaat acaaagccaa aataaaacgc tataaaacaa acctttaaat tttttatata
     4021 aactaaacaa ttttacctct ttacttgagg tgactaaagt ttataggggt aaattattct
     4081 ttcacctcat ataaattccc caaattttta aatagacact tcataaaaaa atcctcctaa
     4141 attagaatag tttaaatata tcatttttgt tcagtaatat taatatgtac actatttcca
     4201 aaatttaaat tcatgttgcc aaaaatcgat tatgtctttt gcggggcaaa aaaagggcag
     4261 attatttgaa aaagggcaaa cgcttgtgga aaagctaaaa ggttaaaaat gacaaaaacc
     4321 ttgatacaac agtgtttttg gacgctcgtg tacgttagag aatgaccggt ttgatatgcc
     4381 tcctaaattt ttatctaaag tgaatttagg aggcttactt gtctgctttc ttcattagaa
     4441 tcaatccttt tttaaaagtc aatattactg taacataaat atatatttta aaaatatccc
     4501 actttatcca attttcgttt gttgaactaa tgggtgcttt agttgaagaa taaaagacca
     4561 cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg aaaaatcctt
     4621 ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt gctgaaaggt
     4681 gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa attggttgca
     4741 cagaaaaacc ccatctgtta aagttataag tgactaaaca aataactaaa tagatggggg
     4801 tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa tcaagggttt
     4861 tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa atcgctaatg
     4921 ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga gtaaaagatt
     4981 gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag ttgtatcgag
     5041 tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca atgaaacatg
     5101 gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca gttcgttggt
     5161 tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat aagagtttgt
     5221 cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat aaaaatcttg
     5281 ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct tataatcagc
     5341 acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa aactacgtga
     5401 atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat gatccaaatg
     5461 taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa tcggcaattg
     5521 acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat gaagaaaaga
     5581 atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta atctcctatg
     5641 gtggtttgtt aaaagaaata cataaaaaat taaaccttga tgacacagaa gaaggcgatt
     5701 tgattcatac agatgatgac gaaaaagccg atgaagatgg attttctatt attgcaatgt
     5761 ggaattggga acggaaaaat tattttatta aagagtagtt caacaaacgg gccagtttgt
     5821 tgaagattag atgctataat tgttattaaa aggattgaag gatgcttagg aagacgagtt
     5881 attaatagct gaataagaac ggtgctctcc aaatattctt atttagaaaa gcaaatctaa
     5941 aattatctga aaagggaatg agaatagtga atggaccaat aataatgact agagaagaaa
     6001 gaatgaagat tgttcatgaa attaaggaac gaatattgga taaatatggg gatgatgtta
     6061 aggctattgg tgtttatggc tctcttggtc gtcagactga tgggccctat tcggatattg
     6121 agatgatgtg tgtcatgtca acagaggaag cagagttcag ccatgaatgg acaaccggaa
     6181 agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat atgagttatg
     6241 cagtttgtag aatgcaaaaa gtgaaatcag ctgcctcgcg cgtttcggtg atgacggtga
     6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     6481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7801 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8101 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8461 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat c
//
