LOCUS       V014922                 6433 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014922
VERSION     V014922
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             323..997
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     CDS             complement(1564..2211)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             2716..3717
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    4334..4474
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4660..5248)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5422..6279)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6280..6384)
                     /label="AmpR promoter"
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
       61 gaaaaatata cctgtatctt ttttcaaaag caaacatgct ttgggtaaac atgtaggtat
      121 taacgtcaat gcgacaatag tagcattgat taaatgagaa ttagtaagtg ttttacttac
      181 taaattttat ttaacctaaa aatgaaccac ctggatgtgt gggattaaaa agtgaagaga
      241 ggaggacata tcacttagga ggatgattat ttgagctcct ctggctaact agagaaccca
      301 ctgcttactg gcttatcgaa atatggcttc ctccgaagac gttatcaaag agttcatgcg
      361 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      421 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      481 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      541 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      601 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      661 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgatgg
      721 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      781 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      841 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      901 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      961 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa ggatcctcta gagtcgacct
     1021 gcaggcatgc aagcttctgt aggtttttag gcataaaact atatgattta cccctaaatc
     1081 tttaaaatgc cccttaaaat tcaaaataaa ggcatttaaa atttaaatat ttcttgtgat
     1141 aaagtttgtt aaaaaggagt ggttttatga ctgttatgtg gttatcgatt ataggtatgt
     1201 ggttttgtat tggaatggca ttttttgcta tcaaggttat taaaaataaa aattagacca
     1261 cgcatttatg ccgagaaaat ttattgtgcg ttgagaagaa cccttaacta aacttgcaga
     1321 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
     1381 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gaagcgaaaa aggagaagtc
     1441 ggttcagaaa aagaaggata tggatctgga gctgtaatat aaaaaccttc ttcaactaac
     1501 ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata
     1561 ttataaaagc cagtcattag gcctatctga caattcctga atagagttca taaacaatcc
     1621 tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga
     1681 attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt
     1741 attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc
     1801 attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc
     1861 agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga
     1921 gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc
     1981 taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt
     2041 cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa
     2101 aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt
     2161 aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc
     2221 ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga
     2281 atcaatcctt tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
     2341 ccactttatc caattttcgt ttgttgaatt tttttaaaag tcaatattac tgtaacataa
     2401 atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaact aatgggtgct
     2461 ttagttgaag aataaaagac cacattaaaa aatgtggtct tttgtgtttt tttaaaggat
     2521 ttgagcgtag cgaaaaatcc ttttctttct tatcttgata catatagaaa taacgtcatt
     2581 tttattttag ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg
     2641 aaaaccctta aaattggttg cacagaaaaa ccccatctgt taaagttata agtgactaaa
     2701 caaataacta aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat
     2761 tcagatgaaa aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg
     2821 agagaaaaga aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa
     2881 aaggctgaaa gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca
     2941 ggcgaaagaa agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac
     3001 tggaggagag caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa
     3061 caaaagccaa cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc
     3121 gaagaattaa ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat
     3181 aaaaaaatta ataaaaatct tgttggtttt atgcgtgcaa cggaagtgac aataaataat
     3241 aaagataatt cttataatca gcacatgcat gtattggtat gtgtggaacc aacttatttt
     3301 aagaatacag aaaactacgt gaatcaaaaa caatggattc aattttggaa aaaggcaatg
     3361 aaattagact atgatccaaa tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa
     3421 tcggatatac aatcggcaat tgacgaaact gcaaaatatc ctgtaaagga tacggatttt
     3481 atgaccgatg atgaagaaaa gaatttgaaa cgtttgtctg atttggagga aggtttacac
     3541 cgtaaaaggt taatctccta tggtggtttg ttaaaagaaa tacataaaaa attaaacctt
     3601 gatgacacag aagaaggcga tttgattcat acagatgatg acgaaaaagc cgatgaagat
     3661 ggattttcta ttattgcaat gtggaattgg gaacggaaaa attattttat taaagagtag
     3721 ttcaacaaac gggccagttt gttgaagatt agatgctata attgttatta aaaggattga
     3781 aggatgctta ggaagacgag ttattaatag ctgaataaga acggtgctct ccaaatattc
     3841 ttatttagaa aagcaaatct aaaattatct gaaaagggaa tgagaatagt gaatggacca
     3901 ataataatga ctagagaaga aagaatgaag attgttcatg aaattaagga acgaatattg
     3961 gataaatatg gggatgatgt taaggctatt ggtgtttatg gctctcttgg tcgtcagact
     4021 gatgggccct attcggatat tgagatgatg tgtgtcatgt caacagagga agcagagttc
     4081 agccatgaat ggacaaccgg aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg
     4141 agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agctgcctcg
     4201 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4261 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4321 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     4381 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4441 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     4501 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4561 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4621 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4681 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4741 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4801 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4861 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4921 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4981 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5041 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5101 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5161 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5221 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5281 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5341 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5401 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5461 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5521 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5581 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5641 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5701 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     5761 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5821 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5881 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5941 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6001 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     6061 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6121 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6181 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6241 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6301 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6421 aaccattatt atc
//
