LOCUS       V015088                 8809 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015088
VERSION     V015088
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             302..3373
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             complement(3940..4587)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             5092..6093
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    6710..6850
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7036..7624)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7798..8655)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8656..8760)
                     /label="AmpR promoter"
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
       61 gaaaaatata cctgtatctt ttttcaaaag caaacatgct ttgggtaaac atgtaggtat
      121 taacgtcaat gcgacaatag tagcattgat taaatgagaa ttagtaagtg ttttacttac
      181 taaattttat ttaacctaaa aatgaaccac ctggatgtgt gggattaaaa agtgaagaga
      241 ggaggacata tcactaggcg atggagttcg ccatagtcta tttttttagg aggaaggatc
      301 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      481 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
      541 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
      601 ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
      661 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
      721 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
      781 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
      841 cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt atctggaaga
      901 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac
      961 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt
     1021 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc
     1081 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
     1141 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa
     1201 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga
     1261 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
     1321 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca
     1381 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
     1441 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta
     1501 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg
     1561 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg
     1621 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
     1681 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga
     1741 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
     1801 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
     1861 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
     1921 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
     1981 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     2041 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
     2101 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga
     2161 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
     2221 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga
     2281 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt
     2341 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg
     2401 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca
     2461 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca
     2521 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa
     2581 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc
     2641 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
     2701 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
     2761 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
     2821 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
     2881 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
     2941 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
     3001 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     3061 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
     3121 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat
     3181 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga
     3241 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
     3301 gagcccgtca gtatcggcgg aattacagct gagcgccggt cgctaccatt accagttggt
     3361 ctggtgtcaa aaataaggat cctctagagt cgacctgcag gcatgcaagc ttctgtaggt
     3421 ttttaggcat aaaactatat gatttacccc taaatcttta aaatgcccct taaaattcaa
     3481 aataaaggca tttaaaattt aaatatttct tgtgataaag tttgttaaaa aggagtggtt
     3541 ttatgactgt tatgtggtta tcgattatag gtatgtggtt ttgtattgga atggcatttt
     3601 ttgctatcaa ggttattaaa aataaaaatt agaccacgca tttatgccga gaaaatttat
     3661 tgtgcgttga gaagaaccct taactaaact tgcagacgaa tgtcggcata gcgtgagcta
     3721 ttaagccgac cattcgacaa gttttgggat tgttaagggt tccgaggctc aacgtcaata
     3781 aagcaattgg aataaagaag cgaaaaagga gaagtcggtt cagaaaaaga aggatatgga
     3841 tctggagctg taatataaaa accttcttca actaacgggg caggttagtg acattagaaa
     3901 accgactgta aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct
     3961 atctgacaat tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga
     4021 atgatgtacc tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc
     4081 tgctgtaata atgggtagaa ggtaattact attattattg atatttaagt taaacccagt
     4141 aaatgaagtc catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg
     4201 aaacaatttc cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc
     4261 tttgaagtca ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa
     4321 aattgtataa agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc
     4381 agttctaaaa gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta
     4441 aaatttatat ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt
     4501 tatactaaaa gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt
     4561 gtctaaatca attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga
     4621 atttaggagg cttacttgtc tgctttcttc attagaatca atcctttttt taaaagtcaa
     4681 tattactgta acataaatat atattttaaa aatatcccac tttatccaat tttcgtttgt
     4741 tgaatttttt taaaagtcaa tattactgta acataaatat atattttaaa aatatcccac
     4801 tttatccaat tttcgtttgt tgaactaatg ggtgctttag ttgaagaata aaagaccaca
     4861 ttaaaaaatg tggtcttttg tgttttttta aaggatttga gcgtagcgaa aaatcctttt
     4921 ctttcttatc ttgatacata tagaaataac gtcattttta ttttagttgc tgaaaggtgc
     4981 gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca
     5041 gaaaaacccc atctgttaaa gttataagtg actaaacaaa taactaaata gatgggggtt
     5101 tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc aagggtttta
     5161 gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt
     5221 gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt
     5281 gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg
     5341 tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat gaaacatggc
     5401 attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt tcgttggttg
     5461 tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa gagtttgtca
     5521 gatatggctc aaggatttcg ccgaatgatg caatataaaa aaattaataa aaatcttgtt
     5581 ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta taatcagcac
     5641 atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa ctacgtgaat
     5701 caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga tccaaatgta
     5761 aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc ggcaattgac
     5821 gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga agaaaagaat
     5881 ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat ctcctatggt
     5941 ggtttgttaa aagaaataca taaaaaatta aaccttgatg acacagaaga aggcgatttg
     6001 attcatacag atgatgacga aaaagccgat gaagatggat tttctattat tgcaatgtgg
     6061 aattgggaac ggaaaaatta ttttattaaa gagtagttca acaaacgggc cagtttgttg
     6121 aagattagat gctataattg ttattaaaag gattgaagga tgcttaggaa gacgagttat
     6181 taatagctga ataagaacgg tgctctccaa atattcttat ttagaaaagc aaatctaaaa
     6241 ttatctgaaa agggaatgag aatagtgaat ggaccaataa taatgactag agaagaaaga
     6301 atgaagattg ttcatgaaat taaggaacga atattggata aatatgggga tgatgttaag
     6361 gctattggtg tttatggctc tcttggtcgt cagactgatg ggccctattc ggatattgag
     6421 atgatgtgtg tcatgtcaac agaggaagca gagttcagcc atgaatggac aaccggaaag
     6481 agcgaaaatg cctcacattt gtgccaccta aaaaggagcg atttacatat gagttatgca
     6541 gtttgtagaa tgcaaaaagt gaaatcagct gcctcgcgcg tttcggtgat gacggtgaaa
     6601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6721 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6781 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6841 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7441 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7801 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7861 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7921 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7981 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8041 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8101 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8161 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8221 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8281 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8341 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8401 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8461 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8521 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8581 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8641 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8701 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8761 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatc
//
