LOCUS       40924_28277        7674 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLHBA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 7674)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7674)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..103
                     /note="multiple cloning site from vector pBlueSfi BA in
                     GenBank Accession Number AF327875"
     regulatory      9..18
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 129
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    148..172
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   196..208
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 202
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   231..242
                     /label=repeat C
                     /note="repeat C"
     repeat_region   256..266
                     /label=repeat B
                     /note="repeat B"
     repeat_region   283..293
                     /label=repeat B
                     /note="repeat B"
     misc_difference 288
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 299
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(316..706)
                     /note="3' sequence of the ornithine cyclodeaminase gene
                     ocd; non-functional"
     misc_difference 492
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      817..1405
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 1503..1504
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(1590..1730)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      2018..2023
                     /regulatory_class="minus_35_signal"
     regulatory      2044..2049
                     /regulatory_class="minus_10_signal"
     regulatory      2066..2070
                     /regulatory_class="ribosome_binding_site"
     CDS             2080..2766
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAO85341.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 2499
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       2910..2942
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 2918
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 2933
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      3000..3005
                     /regulatory_class="minus_35_signal"
     regulatory      3021..3026
                     /regulatory_class="minus_10_signal"
     regulatory      3054..3061
                     /regulatory_class="ribosome_binding_site"
     CDS             3065..3691
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    3961..3973
                     /note="identical to the korB operator binding site of
                     plasmid RK2 in GenBank Accession Number L27758.1"
     CDS             4123..5193
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       5210..5232
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      5262..5456
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   5553..5566
                     /label=repeat E
                     /rpt_unit_range=5553 .. 5559
                     /note="repeat E"
     regulatory      5797..5802
                     /regulatory_class="minus_35_signal"
     regulatory      5820..5825
                     /regulatory_class="minus_10_signal"
     CDS             6061..6849
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       6855..6912
                     /note="palindromic sequence; putative termination signal"
     misc_difference 7089..7090
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    7372..7396
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaggcc atggcggccg cactagtgga tcccccggga tgcatgaatt caagcttatc
       61 gatgtcgaca ggccttaagg gccagatctt gggcccggta cccgatcaga ttgtcgtttc
      121 ccgccttcgg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
      181 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
      241 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc
      301 gataatgact tctgttcaac cacccaaacg tcggaaagcc tgacgacgga gcagcattcc
      361 aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga
      421 tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac
      481 gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg acgtcctggc cagtcatcac
      541 gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc tggatctcgc cttcaatccg
      601 ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc
      661 ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt
      721 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      961 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1381 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1441 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1501 ccgattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1561 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     1621 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1681 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     1741 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1801 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1861 gcgcgaggca ggggtacgtc gaggtcgatc caacccctcc gctgctatag tgcagtcggc
     1921 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     1981 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     2041 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     2101 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     2161 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     2221 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     2281 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     2341 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     2401 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     2461 atcgaccgca cccggagcgg gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc
     2521 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     2581 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     2641 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     2701 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     2761 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     2821 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     2881 tgcatgaaat cctggccggt ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg
     2941 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     3001 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     3061 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     3121 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     3181 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     3241 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     3301 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     3361 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     3421 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     3481 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     3541 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     3601 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     3661 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     3721 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     3781 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     3841 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     3901 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     3961 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     4021 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     4081 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     4141 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg
     4201 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     4261 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     4321 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     4381 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     4441 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     4501 gcagggccgg ccggcatggc gagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     4561 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     4621 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     4681 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     4741 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     4801 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     4861 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     4921 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     4981 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     5041 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     5101 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     5161 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     5221 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     5281 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     5341 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     5401 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     5461 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     5521 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     5581 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     5641 cgcccacatc aaggcaccgg tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc
     5701 tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga
     5761 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     5821 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     5881 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     5941 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     6001 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     6061 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     6121 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     6181 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     6241 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     6301 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     6361 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     6421 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     6481 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     6541 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     6601 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     6661 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     6721 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     6781 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     6841 gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     6901 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     6961 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa
     7021 aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat agcttgcttg
     7081 gtcgttccgc gtgaacgtcg gctcgattgt acctgcgttc aaatactttg cgatcgtgtt
     7141 gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac tgcttctctc gcaacgccat
     7201 ccgacggatg atgtttaaaa gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg
     7261 ttggggggaa ggtgcacatg gctcagttct caatggaaat tatctgccta accggctcag
     7321 ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa agcggcagcg gcggcaggat
     7381 atattcaatt gtaaatggct tcatgtccgg gaaatctaca tggatcagca atgagtatga
     7441 tggtcaatat ggagaaaaag aaagagtaat taccaatttt ttttcaattc aaaaatgtag
     7501 atgtccgcag cgttattata aaatgaaagt acattttgat aaaacgacaa attacgatcc
     7561 gtcgtattta taggcgaaag caataaacaa attattctaa ttcggaaatc tttatttcga
     7621 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggc
//
