LOCUS       40924_28267        9146 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLH9500, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1474 to 1575)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING 
            OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; Gesellschaft fuer 
            Pflanzenzuechtung e. V., Von Sieboldstr. 8, Goettingen, Germany 
            (1999)
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2001) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 9146)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: " 
            BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
            Gesellschaft fuer Pflanzenzuechtung e. V., Von Sieboldstr. 8, 
            Goettingen, Germany (1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2001) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             439..1239
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     regulatory      1256..1455
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus; GenBank Accession Number: X05868, M31331"
                     /regulatory_class="terminator"
     misc_difference 1289
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1421..1436
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    1451
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     misc_feature    1474..1575
                     /note="multiple cloning site from vector pBlueSfi AB;
                     GenBank Accession Number: AF327874"
                     /citation=[1]
     regulatory      1545..1554
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 1601
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1620..1644
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   1668..1680
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 1674
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   1703..1714
                     /label=repeat C
                     /note="repeat C"
     repeat_region   1728..1738
                     /label=repeat B'
                     /note="repeat B'"
     repeat_region   1755..1765
                     /label=repeat B
                     /note="repeat B"
     misc_difference 1760
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 1771
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1788..2178
                     /note="3' sequence of the ornithine cyclodeaminase gene ocd
                     (non-functional)"
     misc_difference 1964
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      2289..2877
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 2975..2976
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(3062..3202)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      3490..3495
                     /regulatory_class="minus_35_signal"
     regulatory      3516..3521
                     /regulatory_class="minus_10_signal"
     regulatory      3538..3542
                     /regulatory_class="ribosome_binding_site"
     CDS             3552..4238
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase (GenBank Accession Number 
                     V00613), Tn917 resolvase (GenBank Accession Number M11180) 
                     and ParA (RK2) (GenBank Accession Number L27758)"
                     /protein_id="AAL78966.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 3971
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       4382..4414
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 4390
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 4405
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      4472..4477
                     /regulatory_class="minus_35_signal"
     regulatory      4493..4498
                     /regulatory_class="minus_10_signal"
     regulatory      4526..4533
                     /regulatory_class="ribosome_binding_site"
     CDS             4537..5163
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5433..5445
                     /note="identical to the korB operator binding site of
                     plasmid RK2 (Pansegrau et al. 1994, J. Mol. Biol. 239: 
                     623-663); GenBank Accession Number L27758"
     CDS             5595..6665
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       6682..6704
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      6734..6928
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   7025..7031
                     /label=repeat E
                     /note="repeat E"
     repeat_region   7032..7038
                     /label=repeat E
                     /note="repeat E"
     regulatory      7269..7274
                     /regulatory_class="minus_35_signal"
     regulatory      7292..7297
                     /regulatory_class="minus_10_signal"
     CDS             7533..8321
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       8327..8384
                     /note="palindromic sequence; putative termination signal"
     misc_difference 8561..8562
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    8844..8868
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      481 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      541 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      601 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      661 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      721 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      781 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      841 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      901 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      961 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     1021 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     1081 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1141 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1201 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1261 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1321 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1381 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1441 aaaccaaaat ccagtgacct gcaggcatgc aagctagagg cccttaaggc cttactagtg
     1501 gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga cgcggccgcc atggccagat
     1561 ctgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt
     1621 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     1681 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     1741 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa
     1801 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg
     1861 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc
     1921 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg
     1981 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct
     2041 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata
     2101 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg
     2161 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2941 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata
     3001 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3061 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     3121 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3181 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga
     3361 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     3421 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     3481 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     3541 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     3601 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     3661 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     3721 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     3781 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     3841 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     3901 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     3961 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     4021 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     4081 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     4141 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     4201 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     4261 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     4321 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     4381 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct
     4441 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     4501 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     4561 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     4621 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     4681 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     4741 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     4801 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     4861 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     4921 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     4981 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     5041 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     5101 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     5161 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     5221 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     5281 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     5341 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     5401 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     5461 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     5521 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
     5581 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     5641 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg
     5701 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     5761 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     5821 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     5881 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     5941 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt
     6001 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     6061 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     6121 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     6181 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     6241 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     6301 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     6361 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     6421 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     6481 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     6541 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     6601 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     6661 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     6721 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     6781 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     6841 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     6901 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     6961 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     7021 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     7081 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg
     7141 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct
     7201 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     7261 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     7321 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     7381 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     7441 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     7501 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     7561 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     7621 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     7681 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     7741 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     7801 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     7861 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     7921 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     7981 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     8041 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     8101 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     8161 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     8221 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     8281 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt
     8341 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     8401 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     8461 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata
     8521 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt
     8581 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc
     8641 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg
     8701 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt
     8761 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc
     8821 caccgggtgc aaagcggcag cggcggcagg atatattcaa ttgtaaatgg cttcatgtcc
     8881 gggaaatcta catggatcag caatgagtat gatggtcaat atggagaaaa agaaagagta
     8941 attaccaatt ttttttcaat tcaaaaatgt agatgtccgc agcgttatta taaaatgaaa
     9001 gtacattttg ataaaacgac aaattacgat ccgtcgtatt tataggcgaa agcaataaac
     9061 aaattattct aattcggaaa tctttatttc gacgtgtcta cattcacgtc caaatggggg
     9121 cttagatgag aaacttcacg atcggc
//
