LOCUS       40924_28262        9154 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLH9000, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1482 to 1583)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING 
            OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; Gesellschaft fuer 
            Pflanzenzuechtung e. V., Von Sieboldstr. 8, Goettingen, Germany 
            (1999)
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2001) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 9154)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: " 
            BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
            Gesellschaft fuer Pflanzenzuechtung e. V., Von Sieboldstr. 8, 
            Goettingen, Germany (1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2001) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             439..1239
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     regulatory      1256..1455
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus; GenBank Accession Number: X05868, M31331"
                     /regulatory_class="terminator"
     misc_difference 1289
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1421..1436
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    1451
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     misc_feature    1482..1583
                     /note="multiple cloning site from vector pBlueSfi BA;
                     GenBank Accession Number: AF327875"
                     /citation=[1]
     regulatory      1489..1498
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 1609
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1628..1652
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   1676..1688
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 1682
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   1711..1722
                     /label=repeat C
                     /note="repeat C"
     repeat_region   1736..1746
                     /label=repeat B
                     /note="repeat B"
     repeat_region   1763..1773
                     /label=repeat B
                     /note="repeat B"
     misc_difference 1768
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 1779
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1796..2186
                     /note="3' sequence of the ornithine cyclodeaminase gene ocd
                     (non-functional)"
     misc_difference 1972
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      2297..2885
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 2983..2984
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(3070..3210)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      3498..3503
                     /regulatory_class="minus_35_signal"
     regulatory      3524..3529
                     /regulatory_class="minus_10_signal"
     regulatory      3546..3550
                     /regulatory_class="ribosome_binding_site"
     CDS             3560..4246
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase (GenBank Accession Number 
                     V00613), Tn917 resolvase (GenBank Accession Number M11180) 
                     and ParA (RK2) (GenBank Accession Number L27758)"
                     /protein_id="AAL78961.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 3979
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       4390..4422
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 4398
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 4413
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      4480..4485
                     /regulatory_class="minus_35_signal"
     regulatory      4501..4506
                     /regulatory_class="minus_10_signal"
     regulatory      4534..4541
                     /regulatory_class="ribosome_binding_site"
     CDS             4545..5171
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5441..5453
                     /note="identical to the korB operator binding site of
                     plasmid RK2 (Pansegrau et al. 1994, J. Mol. Biol. 239: 
                     623-663); GenBank Accession Number L27758"
     CDS             5603..6673
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       6690..6712
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      6742..6936
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   7033..7039
                     /label=repeat E
                     /note="repeat E"
     repeat_region   7040..7046
                     /label=repeat E
                     /note="repeat E"
     regulatory      7277..7282
                     /regulatory_class="minus_35_signal"
     regulatory      7300..7305
                     /regulatory_class="minus_10_signal"
     CDS             7541..8329
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       8335..8392
                     /note="palindromic sequence; putative termination signal"
     misc_difference 8569..8570
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    8852..8876
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      481 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      541 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      601 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      661 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      721 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      781 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      841 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      901 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      961 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     1021 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     1081 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1141 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1201 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1261 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1321 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1381 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1441 aaaccaaaat ccagtgacct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     1501 cactagtgga tcccccggga tgcatgaatt caagcttatc gatgtcgaca ggccttaagg
     1561 gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat
     1621 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     1681 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     1741 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac
     1801 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt
     1861 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga
     1921 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct
     1981 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag
     2041 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca
     2101 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc
     2161 cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt tttgataatc
     2221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg cctttgagtg
     3001 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3061 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3121 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     3181 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     3241 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3301 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc
     3361 gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     3421 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     3481 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     3541 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     3601 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     3661 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     3721 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     3781 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     3841 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     3901 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     3961 gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     4021 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     4081 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     4141 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     4201 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     4261 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     4321 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     4381 ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc
     4441 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     4501 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     4561 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     4621 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     4681 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     4741 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     4801 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     4861 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     4921 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     4981 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     5041 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     5101 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     5161 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     5221 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     5281 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     5341 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     5401 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     5461 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     5521 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     5581 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     5641 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg cccagcggca
     5701 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     5761 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     5821 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     5881 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     5941 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     6001 gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     6061 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     6121 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     6181 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     6241 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     6301 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6361 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6421 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     6481 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     6541 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6601 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6661 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     6721 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     6781 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     6841 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     6901 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     6961 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     7021 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     7081 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg
     7141 tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     7201 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     7261 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     7321 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     7381 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     7441 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     7501 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     7561 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     7621 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     7681 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     7741 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     7801 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     7861 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     7921 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     7981 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     8041 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     8101 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     8161 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     8221 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     8281 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     8341 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     8401 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     8461 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     8521 tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg
     8581 gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc
     8641 ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg atgtttaaaa
     8701 gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg
     8761 gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag aaaccaacat
     8821 gcaagctcca ccgggtgcaa agcggcagcg gcggcaggat atattcaatt gtaaatggct
     8881 tcatgtccgg gaaatctaca tggatcagca atgagtatga tggtcaatat ggagaaaaag
     8941 aaagagtaat taccaatttt ttttcaattc aaaaatgtag atgtccgcag cgttattata
     9001 aaatgaaagt acattttgat aaaacgacaa attacgatcc gtcgtattta taggcgaaag
     9061 caataaacaa attattctaa ttcggaaatc tttatttcga cgtgtctaca ttcacgtcca
     9121 aatgggggct tagatgagaa acttcacgat cggc
//
