LOCUS       40924_28257        8903 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLH7500, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING 
            OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; GPZ e. V., Von 
            Sieboldstr. 8, Goettingen, Germany (1999)
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 8903)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: " 
            BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
            GPZ e. V., Von Sieboldstr. 8, Goettingen, Germany (1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             439..987
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     regulatory      1013..1212
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus in GenBank Accession Numbers X05868 and 
                     V00140"
                     /regulatory_class="terminator"
     misc_difference 1051
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1178..1193
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    1208
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     misc_feature    1231..1332
                     /note="multiple cloning site from vector pBlueSfi AB in
                     GenBank Accession Number AF327874"
     regulatory      1302..1311
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 1358
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1377..1401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   1425..1437
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 1431
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   1460..1471
                     /label=repeat C
                     /note="repeat C"
     repeat_region   1485..1495
                     /label=repeat B
                     /note="repeat B"
     repeat_region   1512..1522
                     /label=repeat B
                     /note="repeat B"
     misc_difference 1517
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 1528
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(1545..1935)
                     /note="3' sequence of the ornithine cyclodeaminase gene
                     ocd; non-functional"
     misc_difference 1721
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      2046..2634
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 2732..2733
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(2819..2959)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      3247..3252
                     /regulatory_class="minus_35_signal"
     regulatory      3273..3278
                     /regulatory_class="minus_10_signal"
     regulatory      3295..3299
                     /regulatory_class="ribosome_binding_site"
     CDS             3309..3995
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAO85366.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 3728
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       4139..4171
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 4147
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 4162
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      4229..4234
                     /regulatory_class="minus_35_signal"
     regulatory      4250..4255
                     /regulatory_class="minus_10_signal"
     regulatory      4283..4290
                     /regulatory_class="ribosome_binding_site"
     CDS             4294..4920
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5190..5202
                     /note="identical to the korB operator binding site of
                     plasmid RK2 in GenBank Accession Number L27758.1"
     CDS             5352..6422
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       6439..6461
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      6491..6685
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   6782..6795
                     /label=repeat E
                     /rpt_unit_range=6782 .. 6788
                     /note="repeat E"
     regulatory      7026..7031
                     /regulatory_class="minus_35_signal"
     regulatory      7049..7054
                     /regulatory_class="minus_10_signal"
     CDS             7290..8078
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       8084..8141
                     /note="palindromic sequence; putative termination signal"
     misc_difference 8318..8319
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    8601..8625
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc
      481 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
      541 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
      601 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
      661 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc
      721 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg
      781 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg
      841 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag
      901 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc
      961 cgtccggtcc tgcccgtcac cgagatctga gatcacgcgt tctagtccgc aaaaatcacc
     1021 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
     1081 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     1141 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     1201 ccaaaatcca gtgacctgca ggcatgcaag ctagaggccc ttaaggcctt actagtggat
     1261 cccccgggat gcatgaattc aagcttatcg atgtcgacgc ggccgccatg gccagatctg
     1321 ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac
     1381 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     1441 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     1501 tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt
     1561 cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct
     1621 agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc
     1681 gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc
     1741 tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc
     1801 gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc
     1861 gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg
     1921 ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2701 cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga gctgataccg
     2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2881 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2941 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3001 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3061 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc
     3121 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     3181 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     3241 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     3301 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     3361 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     3421 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     3481 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     3541 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     3601 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     3661 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     3721 ccaaggcgcg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     3781 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     3841 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     3901 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     3961 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     4021 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     4081 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     4141 caagctcgcg gcctggccgg cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     4201 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     4261 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     4321 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     4381 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     4441 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     4501 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     4561 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     4621 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     4681 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     4741 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     4801 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     4861 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     4921 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     4981 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     5041 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     5101 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     5161 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     5221 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     5281 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     5341 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5401 gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg
     5461 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     5521 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     5581 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     5641 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     5701 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcg agtgtgtggg
     5761 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     5821 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     5881 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     5941 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     6001 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     6061 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6121 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6181 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6241 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6301 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6361 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6421 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     6481 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     6541 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     6601 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     6661 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     6721 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     6781 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     6841 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccggt gggtatgcct
     6901 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga
     6961 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     7021 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     7081 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     7141 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     7201 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     7261 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     7321 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     7381 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     7441 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     7501 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     7561 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     7621 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     7681 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     7741 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7801 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7861 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7921 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7981 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     8041 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa
     8101 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     8161 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     8221 cctacacaaa ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt
     8281 ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta
     8341 cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca
     8401 cggatcgact gcttctctcg caacgccatc cgacggatga tgtttaaaag tcccatgtgg
     8461 atcactccgt tgccccgtcg ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc
     8521 aatggaaatt atctgcctaa ccggctcagt tctgcgtaga aaccaacatg caagctccac
     8581 cgggtgcaaa gcggcagcgg cggcaggata tattcaattg taaatggctt catgtccggg
     8641 aaatctacat ggatcagcaa tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt
     8701 accaattttt tttcaattca aaaatgtaga tgtccgcagc gttattataa aatgaaagta
     8761 cattttgata aaacgacaaa ttacgatccg tcgtatttat aggcgaaagc aataaacaaa
     8821 ttattctaat tcggaaatct ttatttcgac gtgtctacat tcacgtccaa atgggggctt
     8881 agatgagaaa cttcacgatc ggc
//
