LOCUS       40924_28242        9401 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLH6000, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING 
            OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; GPZ e. V., Von 
            Sieboldstr. 8, Goettingen, Germany (1999)
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 9401)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: " 
            BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
            GPZ e. V., Von Sieboldstr. 8, Goettingen, Germany (1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_difference 447
                     /gene="hph"
                     /gene_synonym="hpt; hyg"
                     /replace="g"
                     /label=compared to GenBank accesion Number K01193
                     /note="compared to GenBank accesion Number K01193"
     CDS             452..1474
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      1503..1702
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus in GenBank Accession Numbers X05868 and 
                     V00140"
                     /regulatory_class="terminator"
     misc_difference 1541
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1668..1683
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    1698
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     misc_feature    1729..1830
                     /note="multiple cloning site from vector pBlueSfi BA in
                     GenBank Accession Number AF327875"
     regulatory      1736..1745
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 1856
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1875..1899
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   1923..1935
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 1929
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   1958..1969
                     /label=repeat C
                     /note="repeat C"
     repeat_region   1983..1993
                     /label=repeat B
                     /note="repeat B"
     repeat_region   2010..2020
                     /label=repeat B
                     /note="repeat B"
     misc_difference 2015
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 2026
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(2043..2433)
                     /note="3' sequence of the ornithine cyclodeaminase gene
                     ocd; non-functional"
     misc_difference 2219
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      2544..3132
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 3230..3231
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(3317..3457)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      3745..3750
                     /regulatory_class="minus_35_signal"
     regulatory      3771..3776
                     /regulatory_class="minus_10_signal"
     regulatory      3793..3797
                     /regulatory_class="ribosome_binding_site"
     CDS             3807..4493
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAO85351.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 4226
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       4637..4669
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 4645
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 4660
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      4727..4732
                     /regulatory_class="minus_35_signal"
     regulatory      4748..4753
                     /regulatory_class="minus_10_signal"
     regulatory      4781..4788
                     /regulatory_class="ribosome_binding_site"
     CDS             4792..5418
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5688..5700
                     /note="identical to the korB operator binding site of
                     plasmid RK2 in GenBank Accession Number L27758.1"
     CDS             5850..6920
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       6937..6959
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      6989..7183
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   7280..7293
                     /label=repeat E
                     /rpt_unit_range=7280 .. 7286
                     /note="repeat E"
     regulatory      7524..7529
                     /regulatory_class="minus_35_signal"
     regulatory      7547..7552
                     /regulatory_class="minus_10_signal"
     CDS             7788..8576
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       8582..8639
                     /note="palindromic sequence; putative termination signal"
     misc_difference 8816..8817
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    9099..9123
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag ggccccgggg ggcaataaga tatgaaaaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      901 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      961 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1021 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1081 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1141 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1201 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1261 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1321 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1381 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1441 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cggagtccgc
     1501 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     1561 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     1621 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     1681 attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccatg
     1741 gcggccgcac tagtggatcc cccgggatgc atgaattcaa gcttatcgat gtcgacaggc
     1801 cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt
     1861 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     1921 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     1981 gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct
     2041 gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt
     2101 ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg
     2161 tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc
     2221 gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc
     2281 gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg
     2341 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg
     2401 cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt
     2461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct
     3241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3301 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3361 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3421 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3481 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3541 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     3601 gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     3661 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     3721 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     3781 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     3841 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     3901 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     3961 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     4021 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     4081 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     4141 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     4201 ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca
     4261 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     4321 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     4381 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     4441 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     4501 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     4561 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     4621 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac
     4681 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     4741 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     4801 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     4861 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     4921 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     4981 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     5041 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     5101 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     5161 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     5221 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     5281 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     5341 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     5401 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     5461 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     5521 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     5581 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     5641 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     5701 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     5761 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     5821 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     5881 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc
     5941 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     6001 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     6061 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     6121 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     6181 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     6241 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     6301 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     6361 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     6421 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     6481 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     6541 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     6601 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     6661 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     6721 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6781 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6841 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6901 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6961 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     7021 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     7081 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     7141 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     7201 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     7261 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     7321 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     7381 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
     7441 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     7501 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     7561 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     7621 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     7681 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     7741 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     7801 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     7861 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     7921 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     7981 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     8041 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     8101 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     8161 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     8221 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     8281 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     8341 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     8401 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     8461 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     8521 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     8581 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     8641 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     8701 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt
     8761 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg
     8821 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg
     8881 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg
     8941 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt
     9001 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa
     9061 ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta
     9121 aatggcttca tgtccgggaa atctacatgg atcagcaatg agtatgatgg tcaatatgga
     9181 gaaaaagaaa gagtaattac caattttttt tcaattcaaa aatgtagatg tccgcagcgt
     9241 tattataaaa tgaaagtaca ttttgataaa acgacaaatt acgatccgtc gtatttatag
     9301 gcgaaagcaa taaacaaatt attctaattc ggaaatcttt atttcgacgt gtctacattc
     9361 acgtccaaat gggggcttag atgagaaact tcacgatcgg c
//
