LOCUS       40924_28237        8958 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLH5000, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING 
            OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; GPZ e. V., Von 
            Sieboldstr. 8, Goettingen, Germany (1999)
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 8958)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: " 
            BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
            GPZ e. V., Von Sieboldstr. 8, Goettingen, Germany (1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             446..1006
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     regulatory      1060..1259
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus in GenBank Accession Numbers X05868 and 
                     V00140"
                     /regulatory_class="terminator"
     misc_difference 1098
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1225..1240
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    1255
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     misc_feature    1286..1387
                     /note="multiple cloning site from vector pBlueSfi BA in
                     GenBank Accession Number AF327875"
     regulatory      1293..1302
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_difference 1413
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    1432..1456
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   1480..1492
                     /label=repeat C'
                     /note="repeat C'"
     misc_difference 1486
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     repeat_region   1515..1526
                     /label=repeat C
                     /note="repeat C"
     repeat_region   1540..1550
                     /label=repeat B
                     /note="repeat B"
     repeat_region   1567..1577
                     /label=repeat B
                     /note="repeat B"
     misc_difference 1572
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 1583
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(1600..1990)
                     /note="3' sequence of the ornithine cyclodeaminase gene
                     ocd; non-functional"
     misc_difference 1776
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      2101..2689
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 2787..2788
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    complement(2874..3014)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     regulatory      3302..3307
                     /regulatory_class="minus_35_signal"
     regulatory      3328..3333
                     /regulatory_class="minus_10_signal"
     regulatory      3350..3354
                     /regulatory_class="ribosome_binding_site"
     CDS             3364..4050
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAO85346.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 3783
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI
                     restriction site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     stem_loop       4194..4226
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 4202
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_difference 4217
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      4284..4289
                     /regulatory_class="minus_35_signal"
     regulatory      4305..4310
                     /regulatory_class="minus_10_signal"
     regulatory      4338..4345
                     /regulatory_class="ribosome_binding_site"
     CDS             4349..4975
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5245..5257
                     /note="identical to the korB operator binding site of
                     plasmid RK2 in GenBank Accession Number L27758.1"
     CDS             5407..6477
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     stem_loop       6494..6516
                     /label=palindromic sequence
                     /note="palindromic sequence"
     rep_origin      6546..6740
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     repeat_region   6837..6850
                     /label=repeat E
                     /rpt_unit_range=6837 .. 6843
                     /note="repeat E"
     regulatory      7081..7086
                     /regulatory_class="minus_35_signal"
     regulatory      7104..7109
                     /regulatory_class="minus_10_signal"
     CDS             7345..8133
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     stem_loop       8139..8196
                     /note="palindromic sequence; putative termination signal"
     misc_difference 8373..8374
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    8656..8680
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag ggccaatttg ccatcatggt tcgaccattg aactgcatcg tcgccgtgtc
      481 ccaaaatatg gggattggca agaacggaga ccgaccctgg cctccgctca ggaacgagtt
      541 caagtacttc caaagaatga ccacaacctc ttcagtggaa ggtaaacaga atctggtgat
      601 tatgggtagg aaaacctggt tctccattcc tgagaagaat cgacctttaa aggacagaat
      661 taatatagtt ctcagtagag aactcaaaga accaccacga ggagctcatt ttcttgccaa
      721 aagtttggat gatgccttaa gacttattga acaaccggaa ttggcaagta aagtagacat
      781 ggtttggata gtcggaggca gttctgttta ccaggaagcc atgaatcaac caggccacct
      841 cagactcttt gtgacaagga tcatgcagga atttgaaagt gacacgtttt tcccagaaat
      901 tgatttgggg aaatataaac ttctcccaga atacccaggc gtcctctctg aggtccagga
      961 ggaaaaaggc atcaagtata agtttgaagt ctacgagaag aaagactaac aggaagatgc
     1021 tttcaagttc tctgctcccc tcctaaagct agtccgcaaa aatcaccagt ctctctctac
     1081 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     1141 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     1201 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     1261 acctgcaggc atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc
     1321 gggatgcatg aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc
     1381 ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat
     1441 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     1501 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     1561 tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa
     1621 agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag
     1681 gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga
     1741 aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg
     1801 gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag
     1861 ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg
     1921 cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat
     1981 gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2221 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2761 gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc
     2821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2881 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2941 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     3001 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3061 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3121 ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc
     3181 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     3241 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     3301 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     3361 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     3421 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     3481 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     3541 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     3601 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     3661 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     3721 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     3781 gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     3841 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     3901 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     3961 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     4021 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     4081 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     4141 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     4201 tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     4261 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     4321 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     4381 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     4441 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     4501 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     4561 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     4621 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     4681 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     4741 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     4801 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     4861 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     4921 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     4981 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     5041 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     5101 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     5161 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     5221 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     5281 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     5341 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     5401 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     5461 gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa
     5521 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     5581 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     5641 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     5701 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     5761 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac
     5821 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     5881 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     5941 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     6001 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     6061 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     6121 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     6181 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     6241 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     6301 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     6361 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     6421 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     6481 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     6541 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     6601 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     6661 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     6721 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     6781 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     6841 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     6901 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga
     6961 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg
     7021 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     7081 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     7141 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     7201 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     7261 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     7321 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     7381 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     7441 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     7501 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     7561 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     7621 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     7681 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     7741 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     7801 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     7861 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     7921 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     7981 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     8041 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     8101 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     8161 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     8221 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     8281 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga
     8341 agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc
     8401 gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat
     8461 cgactgcttc tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac
     8521 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg
     8581 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt
     8641 gcaaagcggc agcggcggca ggatatattc aattgtaaat ggcttcatgt ccgggaaatc
     8701 tacatggatc agcaatgagt atgatggtca atatggagaa aaagaaagag taattaccaa
     8761 ttttttttca attcaaaaat gtagatgtcc gcagcgttat tataaaatga aagtacattt
     8821 tgataaaacg acaaattacg atccgtcgta tttataggcg aaagcaataa acaaattatt
     8881 ctaattcgga aatctttatt tcgacgtgtc tacattcacg tccaaatggg ggcttagatg
     8941 agaaacttca cgatcggc
//
