LOCUS       V016090                 3848 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016090
VERSION     V016090
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..3848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    208..242
                     /label="constitutive promoter J23100"
                     /note="constitutive promoter J23100"
     misc_feature    243..276
                     /label="B0034"
                     /note="B0034"
     misc_feature    277..981
                     /label="ccaR"
                     /note="ccaR"
     misc_feature    427..429
                     /label="D51N"
                     /note="D51N"
     terminator      1002..1073
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1089..1116
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    1123..1227
                     /label="pEXT20 expression vector"
                     /note="pEXT20 expression vector"
     misc_feature    1240..1411
                     /label="PcpcG2-172"
                     /note="PcpcG2-172"
     misc_feature    1344..1365
                     /label="G box"
                     /note="G box"
     misc_feature    1412..1431
                     /label="B0034"
                     /note="B0034"
     misc_feature    1432..1437
                     /label="biobricks suffix"
                     /note="biobricks suffix"
     CDS             1438..2058
                     /gene="rcsA"
                     /label="Transcriptional regulatory protein RcsA"
                     /note="Transcriptional regulatory protein RcsA from
                     Escherichia coli (strain K12). Accession#: P0DMC9"
     terminator      complement(2085..2128)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2398..2986)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3074..3168)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3192..3848)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgaccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgggccgg ccaacgtctc attttcgcca
      121 gatatcgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
      181 acgaggccct ttcgtcttca cctcgagttg acggctagct cagtcctagg tacagtgcta
      241 gctgttaaac gctcctcgta agaacacggg aaagcaatga gaattctttt agtggaggat
      301 gatttgccgc tggcggaaac ccttgctgaa gcattgagtg accagcttta caccgttgat
      361 attgccaccg acgcttccct cgcctgggac tatgcctccc gactggaata tgacctcgtt
      421 attttgaatg tgatgctgcc ggagttggac gggattaccc tctgtcaaaa atggcgatcg
      481 cacagttatt taatgccaat tttgatgatg acagccaggg atacgatcaa tgataaaatc
      541 acgggcttgg atgcgggggc ggatgattat gtggtcaagc cagtggattt gggggagtta
      601 tttgccaggg tgcgagcttt gttgcgtcgg ggttgtgcaa cgtgccaacc agttttagag
      661 tgggggccaa tcaggttgga tccaagcacc tatgaagtta gttatgacaa tgaggttttg
      721 tctttgaccc gcaaggaata cagcattctg gaattactac tccgcaatgg ccgtcgggtg
      781 ctaagtcgga gcatgattat cgatagtatc tggaagttgg agagtccccc agaggaagat
      841 acggttaagg tgcatgtgcg gagtttgcga caaaaattaa aaagtgccgg tttatcagca
      901 gatgccattg aaacggtcca tggcattggg tatcgtctgg ccaatttaac ggaaaaatct
      961 ttgtgccaag ggaaaaacta gtaataatct agaccaggca tcaaataaaa cgaaaggctc
     1021 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1081 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tagtaaatca ctgcataatt
     1141 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg
     1201 ttctggcaaa tattctgaaa tgagctggtt agctagtcaa gcccattgtg cttttctcta
     1261 tcaacctcag cttacctgaa ggggtgaaca ggtctgggtt aattcatgtt gcgaaatgta
     1321 acagttttag tcgcatcagc taactttccg atttctttac gattttctcc cccttttctt
     1381 caattttact ttgttaggat cgcattttta accttagcct aagaggtatc tactagtatg
     1441 tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg
     1501 agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata
     1561 gcttgtgatt cacagcgccc ttcagtggtg tttattaatg aggactgttt catccacgat
     1621 gcttctaaca gtcagcgtat caagctcatc attaatcaac atcccaatac gttatttatc
     1681 gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta
     1741 ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg
     1801 aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc
     1861 gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctctgaccaa
     1921 atgaatatca aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa
     1981 acgcataata aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt
     2041 atttttgtca acatgcgcta ataataatct agaccaggca tcaaataaaa cgaaaggctc
     2101 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2161 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtacccg tggatcctct
     2221 agagtcgacc tgcaggcatg caagcttcct aggcgttcgg ctgcggcgag cggtatcagc
     2281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2581 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2821 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3061 gactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     3121 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     3181 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     3241 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
     3301 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     3361 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     3421 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     3481 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     3541 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     3601 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     3661 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     3721 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     3781 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     3841 ttctccat
//
