LOCUS       V016314                 6282 bp    DNA     circular SYN 27-JUL-2020
DEFINITION  Exported.
ACCESSION   V016314
VERSION     V016314
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Optogenetic control of gut bacterial metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Hartsough LA, Kotlajich MV, Han B, Lin C-C.J., Gambill L, Wang MC,
            Tabor JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Bioengineering, Rice University, 6500 Main
            St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2019) Bioengineering, Rice University, 6500 Main St.,
            Houston, TX 77030, USA"
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     regulatory      52..63
                     /label="sRBS"
                     /note="sRBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             70..789
                     /gene="pbsA1"
                     /label="Heme oxygenase 1"
                     /note="Heme oxygenase 1 from Synechocystis sp. (strain PCC
                     6803 / Kazusa). Accession#: P72849"
     regulatory      795..806
                     /label="RBS pprotet"
                     /note="RBS pprotet"
                     /regulatory_class="ribosome_binding_site"
     CDS             813..1559
                     /codon_start=1
                     /transl_table=11
                     /product="PcyA"
                     /label="PcyA"
                     /protein_id="QLL27767.1"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPED
                     LGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKGLDILHCVMFPEPLYGLPLFGC
                     DIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSEYCL
                     FIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQKNDKT
                     RRVLEKAFGEAWAERYMSQVLFDVIQ"
     terminator      1574..1645
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1661..1688
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     regulatory      1740..1887
                     /note="specR promoter; from KD13"
                     /regulatory_class="promoter"
     CDS             1888..2676
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      2691..2785
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      2899..3444
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3700..3734
                     /label="constitutive promoter J23106"
                     /note="constitutive promoter J23106"
                     /regulatory_class="promoter"
     misc_feature    3735..3769
                     /label="B0034"
                     /note="B0034"
     CDS             3770..6031
                     /codon_start=1
                     /transl_table=11
                     /product="CcaS"
                     /label="CcaS"
                     /protein_id="QLL27768.1"
                     /translation="MGKFLIPIEFVFLAIAMTCYLWHRQNQERRRIEISIKQQTQRERF
                     INQITQHIRQSLNLETVLNTTVAEVKTLLQVDRVLIYRIWQDGTGSAITESVNANYPSI
                     LGRTFSDEVFPVEYHQAYTKGKVRAINDIDQDDIEICLADFVKQFGVKSKLVVPILQHN
                     RASSLDNESEFPYLWGLLITHQCAFTRPWQPWEVELMKQLANQVAIAIQQSELYEQLQQ
                     LNKDLENRVEKRTQQLAATNQSLRMEISERQKTEAALRHTNHTLQSLIAASPRGIFTLN
                     LADQIQIWNPTAERIFGWTETEIIAHPELLTSNILLEDYQQFKQKVLSGMVSPSLELKC
                     QKKDGSWIEIVLSAAPLLDSEENIAGLVAVVADITEQKRQAEQIRLLQSVVVNTNDAVV
                     ITEAEPIDDPGPRILYVNEAFTKITGYTAEEMLGKTPRVLQGPKTSRTELDRVRQAISQ
                     WQSVTVEVINYRKDGSEFWVEFSLVPVANKTGFYTHWIAVQRDVTERRRTEEVRLALER
                     EKELSRLKTRFFSMASAEFRTPLSTALAAAQLLENSEVAWLDPDKRSRNLHRIQNSVKN
                     MVQLLDDILIINRAEAGKLEFNPNWLDLKLLFQQFIEEIQLSVSDQYYFDFICSAQDTK
                     ALVDERLVRSILSNLLSNAIKYSPGGGQIKIALSLDSEQIIFEVTDQGIGISPEDQKQI
                     FEPFHRGKNVRNITGTGLGLMVAKKCVDLHSGSILLKSAVDQGTTVTICLKRYNHLPRA
                     "
     misc_feature    5369..5371
                     /label="H534A"
                     /note="H534A"
     terminator      complement(6055..6098)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcaca cagaattcat taaagaggag
       61 aaaggtacca tgagtgtcaa cttagcttcc cagttgcggg aagggacgaa aaaatcccac
      121 tccatggcgg agaacgtcgg ctttgtcaaa tgcttcctca agggcgttgt cgagaaaaat
      181 tcctaccgta agctggttgg caatctctac tttgtctaca gtgccatgga agaggaaatg
      241 gcaaaattta aggaccatcc catcctcagc cacatttact tccccgaact caaccgcaaa
      301 caaagcctag agcaagacct gcaattctat tacggctcca actggcggca agaagtgaaa
      361 atttctgccg ctggccaagc ctatgtggac cgagtccggc aagtggccgc tacggcccct
      421 gaattgttgg tggcccattc ctacacccgt tacctggggg atctttccgg cggtcaaatt
      481 ctcaagaaaa ttgcccaaaa tgccatgaat ctccacgatg gtggcacagc tttctatgaa
      541 tttgccgaca ttgatgacga aaaggctttt aaaaatacct accgtcaagc tatgaatgat
      601 ctgcccattg accaagccac cgccgaacgg attgtggatg aagccaatga cgcctttgcc
      661 atgaacatga aaatgttcaa cgaacttgaa ggcaacctga tcaaggcgat cggcattatg
      721 gtgttcaaca gcctcacccg tcgccgcagt caaggcagca ccgaagttgg cctcgccacc
      781 tccgaaggct agttaaagag gagaaaggat ccatggccgt cactgattta agtttgacca
      841 attcttccct gatgcctacg ttgaacccga tgattcaaca gttggccctg gcgatcgccg
      901 ctagttggca aagtttaccc ctcaagccct atcaattgcc ggaggatttg ggctacgtag
      961 aaggccgcct ggaaggggaa aagttagtga ttgaaaatcg gtgctaccaa acgccccagt
     1021 ttcgcaaaat gcatttggag ttggccaagg tgggcaaagg gttggatatt ctccactgtg
     1081 taatgtttcc tgagccttta tacggtctac ctttgtttgg ctgtgacatt gtggccggcc
     1141 ccggtggagt aagtgcggct attgcggatc tatcccccac ccaaagcgat cgccaattgc
     1201 ccgcagcgta ccaaaaatca ttggcagagc taggccagcc agaatttgag caacaacggg
     1261 aattgccccc ctggggagaa atattttctg aatattgttt attcatccgt cccagcaatg
     1321 tcactgaaga agaaagattt gtacaaaggg tagtggactt tttgcaaatt cattgtcacc
     1381 aatccatcgt tgccgaaccc ttgtctgaag ctcaaacttt ggagcaccgt caggggcaaa
     1441 ttcattactg ccaacaacaa cagaaaaatg ataaaacccg tcgggtactg gaaaaagctt
     1501 ttggggaagc ttgggcggaa cggtatatga gccaagtctt atttgatgtt atccaataat
     1561 ctagaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1681 tttctgcgtt tataggtacc cggggatcct ctagagtcga cctgcaggca tgcaagctta
     1741 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     1801 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat
     1861 caagagacag gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact
     1921 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1981 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2041 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2101 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2161 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2221 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2281 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2341 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2401 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2461 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2521 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2581 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2641 tacgtgaaag gcgagatcac caaggtagtc ggcaaataag agctcgcttg gactcctgtt
     2701 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     2761 ccgggcgttt tttattggtg agaatccaag cactagtaac aacttatatc gtatggggct
     2821 gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagtaat attttatctg
     2881 attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     2941 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     3001 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     3061 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     3121 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     3181 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     3241 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     3301 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     3421 tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     3481 gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc
     3541 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcccctagg
     3601 tggagtctgg cctcaaatac aaagtctgca aagtctcgaa atattaaacc agagaataaa
     3661 taattaaacg ccaaaatatt tctagcaaaa accccagttt ttacggctag ctcagtccta
     3721 ggtatagtgc tagctctacc gctccagcaa actttaaggg aggttagcaa tgggcaaatt
     3781 tctaattcca atcgaatttg tttttctggc gatcgccatg acctgttatt tatggcacag
     3841 acaaaaccaa gaacgccgca ggattgaaat tagcatcaag caacaaaccc aacgggaacg
     3901 atttattaac caaattaccc aacatatccg ccaatcttta aacttggaaa cggttttaaa
     3961 taccaccgtc gctgaagtta aaaccctgtt gcaagttgat cgagttctaa tttatcgcat
     4021 ttggcaagat ggcacgggca gcgccattac ggaatcggtg aatgccaatt atcctagtat
     4081 tttagggcgg accttttccg atgaagtttt tcccgttgaa taccatcaag cctacaccaa
     4141 aggtaaagta cgggccatta atgacattga ccaggatgac atagagattt gcctagctga
     4201 tttcgtcaaa caatttggcg tgaaatcaaa attagtagtg cccattcttc aacataatcg
     4261 tgcttcttcc ctagataatg aatcagaatt tccctatctt tgggggctgt taattaccca
     4321 tcaatgtgct tttacccggc catggcaacc gtgggaagtg gagttaatga aacagctagc
     4381 caatcaggtc gcgatcgcca tccaacaatc ggaattatat gagcaattac agcaactcaa
     4441 taaagatttg gaaaaccgag tcgaaaaacg cacccagcaa cttgccgcca ccaatcaatc
     4501 cctaagaatg gaaatcagtg agcgacaaaa aacggaagcc gctctccgcc acactaacca
     4561 tactctgcaa tccctgattg cggcctcccc caggggtatt tttaccctta atttagcaga
     4621 ccaaattcag atttggaatc ctacagcaga acgtattttt ggttggacag aaacagaaat
     4681 tattgcccat ccagaattat taacatccaa cattttgctg gaagattatc agcaatttaa
     4741 acagaaagtt ttatcaggca tggtttcccc tagcctagaa ttaaaatgtc aaaaaaaaga
     4801 tggtagttgg attgaaattg tcctttccgc tgctccccta ttggatagtg aagaaaatat
     4861 tgccggattg gtggcggttg tcgccgatat taccgagcaa aagcggcagg cagaacaaat
     4921 tcgtttgcta caatccgttg tggttaatac taatgatgcg gtggtgatta cggaagcgga
     4981 gcccattgat gatcccgggc cgagaattct ctatgtcaat gaagcattta ctaaaatcac
     5041 cggttatact gctgaagaaa tgctaggcaa aaccccccga gttttacagg gaccaaaaac
     5101 tagtcgcact gaattagata gggtgcggca agccattagt caatggcaat cagttaccgt
     5161 tgaagtgatt aattatcgta aggatggcag tgagttttgg gtggaattta gtctggtgcc
     5221 cgttgccaat aaaacaggtt tttacaccca ttggattgct gtgcaaaggg atgtcactga
     5281 gcgccgacgc acggaggaag tccgcctagc tttagaacgg gaaaaagaat taagccgcct
     5341 aaaaactcgt tttttctcca tggcttccgc agaatttcgt actcccctca gtacggcctt
     5401 agctgctgcc caattactgg aaaattctga agtggcctgg cttgatcccg ataagcgtag
     5461 ccggaactta caccgtattc aaaattccgt gaaaaatatg gtacagctcc tggatgatat
     5521 tttaatcatt aaccgtgccg aagcgggcaa attggaattt aatcctaatt ggttagattt
     5581 gaaattattg ttccagcaat ttatcgaaga aattcaatta agtgtcagtg accaatatta
     5641 ttttgacttt atttgtagcg ctcaagatac gaaggcattg gtggatgaaa ggttagtgcg
     5701 gtctatttta tctaatctgt tatctaatgc gattaaatac tctcccgggg gagggcagat
     5761 taaaattgcc ctaagcctag attcggaaca gattattttt gaagtcaccg accagggcat
     5821 tggcatttcg ccagaggacc aaaagcaaat ttttgaaccc tttcatcggg gcaaaaatgt
     5881 cagaaatatt acgggaacag gactcggttt aatggttgcc aagaaatgtg ttgacttaca
     5941 cagtggcagt atcttgctaa aaagtgcagt tgaccaggga acaacagtta ctatctgttt
     6001 aaaacgctat aaccatttgc ctcgagctta gtaataatct agaccaggca tcaaataaaa
     6061 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     6121 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tagtaaatca
     6181 ctgcataatt cgtgtcgatt aaaccgccta agtcccccag gaaaggggga tataacagta
     6241 tagattttgt cagccttcag cttggcttta ccgtcaaaaa aa
//
