LOCUS       V016710                 8318 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016710
VERSION     V016710
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..200
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        249..759
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             849..1871
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     primer_bind     complement(1898..1914)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             1997..2230
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2302..2436
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2463..2598
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2619..2637)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2647..2663)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2805..3260
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3286..3390
                     /label="AmpR promoter"
     CDS             3391..4248
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4422..5010
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5298..5319
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5334..5364
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5372..5388
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5396..5412
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5433..5451
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5479..5705
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5706..5886
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5933..6058
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6551..6784
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6969..7013
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7162..7203
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        7311..7551
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     CDS             7900..8202
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_RNA        8243..8261
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
ORIGIN
        1 tatggcgtcg ctccaagtgg caccgagtcg gtgctttttt tgaattctcg acctcgagac
       61 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      121 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      181 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
      241 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
      301 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
      361 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
      421 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
      481 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
      541 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
      601 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
      661 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
      721 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc ggatcaagct
      781 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg cagatctgat cccggggggc
      841 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
      901 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
      961 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     1021 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     1081 ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     1141 tcacgttgca agatctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     1201 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     1261 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     1321 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     1381 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     1441 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     1501 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     1561 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     1621 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     1681 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     1741 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     1801 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     1861 gggcaaagga atagagtaga tgccgaccgg gatctatcga taccgtcgac ctcgaggggg
     1921 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     1981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagaaaaga tctgcttttt
     2041 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2221 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     2281 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     2341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2401 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     2461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2521 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2581 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     2641 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     2821 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2881 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2941 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3001 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3061 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3121 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3181 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3241 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3301 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3361 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3421 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3481 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3541 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3601 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3661 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3721 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3781 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3841 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3901 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3961 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4021 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4081 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4141 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4201 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4261 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4321 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4381 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4441 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4501 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4561 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4621 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4681 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4741 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4801 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4861 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4921 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4981 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5041 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5101 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5161 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5221 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5281 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5341 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5401 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5461 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     5521 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     5581 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     5641 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     5701 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5881 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     5941 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6001 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6061 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6121 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     6181 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     6241 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     6301 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     6361 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     6421 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6481 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6541 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6601 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6661 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6721 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6781 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6841 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     6901 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     6961 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7021 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7081 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7141 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     7201 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     7261 ctcgacggta tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat
     7321 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     7381 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     7441 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     7501 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     7561 gtgtcttcga ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     7621 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg
     7681 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     7741 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     7801 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     7861 tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc
     7921 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     7981 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     8041 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     8101 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     8161 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaaccct gcaggacgaa
     8221 gacccgtttg agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
     8281 caaataaggc tagtccgtta tcaacttgga gcagacga
//
