LOCUS       V016709                 8297 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016709
VERSION     V016709
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        241..259
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    741..866
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1359..1592
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1777..1821
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1970..2011
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        2119..2359
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     CDS             2708..3010
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    3046..3064
                     /label="boxB"
                     /note="lambda N peptide binding site consisting of an RNA
                     hairpin within the N-utliization (nut) site of the lambda
                     bacteriophage transcript (Tan and Frankel, 1995)"
     misc_feature    3188..3305
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        3354..3864
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3954..4976
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     primer_bind     complement(5003..5019)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5102..5335
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5407..5541
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5568..5703
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5724..5742)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5752..5768)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5910..6365
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6391..6495
                     /label="AmpR promoter"
     CDS             6496..7353
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7527..8115
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1981 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2041 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2101 gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     2161 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     2221 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     2281 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2341 tatatcttgt ggaaaggacg aaacaccggt gtcttcgagg cttactaaaa gccagataac
     2401 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     2461 gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga
     2521 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     2581 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     2641 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac
     2701 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     2761 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     2821 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     2881 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     2941 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     3001 ctggggaata taaaccctgc aggacgaaga cccgtttgag agctagggcc ctgaagaagg
     3061 gccctagcaa gttcaaataa ggctagtccg ttatcaactt gggccctgaa gaagggccca
     3121 agtggcaccg agtcggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattca
     3181 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     3241 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     3301 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     3361 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     3421 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     3481 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     3541 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     3601 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     3661 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     3721 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     3781 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     3841 cgaatcaccg acctctctcc ccagggggat ccaccggatc aagcttgcca gctggggcgc
     3901 cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa
     3961 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4021 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4081 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4141 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4201 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagatc
     4261 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     4321 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     4381 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     4441 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     4501 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     4561 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     4621 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     4681 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     4741 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     4801 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     4861 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     4921 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     4981 gtagatgccg accgggatct atcgataccg tcgacctcga gggggggccc ggtaccttta
     5041 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5101 ctggaagggc taattcactc ccaacgaaga aaagatctgc tttttgcttg tactgggtct
     5161 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5221 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5281 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5341 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5401 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5521 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5581 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5641 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5701 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5761 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5821 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5881 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6361 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6841 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6901 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6961 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7021 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7081 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7141 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7201 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7261 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7321 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7381 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7441 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7501 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7561 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7621 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7681 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7741 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7801 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7861 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7921 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7981 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8041 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8101 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8161 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8221 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8281 agcgaggaag cggaaga
//
