LOCUS       40924_28177        7493 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLG338, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Mitarai N, Jensen MH, Semsey S.
  TITLE     Coupled positive and negative feedbacks produce diverse gene 
            expression patterns in colonies
  JOURNAL   MBio 6 (2), e00059-15 (2015)
  PUBMED    25852158
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Semsey S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2014) Niels Bohr Institute, University of 
            Copenhagen, Blegdamsvej 17, Copenhagen 2100, Denmark
REFERENCE   3  (bases 1 to 7493)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2015"; volume: "6"; issue: "2"; pages: "e00059-15"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2014) Niels Bohr Institute, University of Copenhagen, 
            Blegdamsvej 17, Copenhagen 2100, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2760..2788
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2836..4023
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      4391..4613
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4661..5608
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELITRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             join(6945..7493,1..264)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
       61 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
      121 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
      181 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
      241 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
      301 agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact tttgctgagt
      361 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
      421 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
      481 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag
      541 gcagacctca gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcgtat
      601 tcaggctgac cctgcgcgct gcgcagggct ttattgattc catttttaca ctgatgaatg
      661 ttccgttgcg ctgcccggat tacagctgtg tcagcaggcg ggcaaagtcg gctgcatcgc
      721 gtccgtctat gcgttttgtg cagcccagaa cggaatctca gcaggcaatg cgggctctgc
      781 atcgtgtccg tgaatccctg gttcaggata aggtaaaaac aaccaatcaa atgcatgctt
      841 ttctgctgga atttggcatt agcgttcccc gaggagctgc cgttattagc cgactgagta
      901 ccattcttga ggataatagt ttgcctcttt acctcagcca gttattgctg aaattacaac
      961 agcattatca ctatcttgtt gagcagatta aagatttgga atcccagttg aaacgaaagt
     1021 tggacgaaga tgaggttgga cagcgcttgc tgagcattcc ctgcgtcgga acactgacag
     1081 cgagtactat ttcaactgag attggcgacg ggaagcagta cgccagcagc cgtgactttg
     1141 cggcggcaac agggcttgta cctcggcagt acagcacggg aggtaggacg acattgctgg
     1201 gaattagtaa gcgaggtaat aaaaagatcc gaactttgtt ggttcaatgt gccagggtat
     1261 tcatacaaaa actggaacac cagtctggca aattggccga ttgggtcagg gatttactgt
     1321 gccggaaaag caactttgtc gtcacttgtg ctctggcaaa caagctggcc agaatagcct
     1381 gggccctaac ggcacgacag caaacttatg tagcataacg gcagaaatac accggtttaa
     1441 agaattactg atctggtttt gcgaatactg atattgatga tactaacggc ccaccggcct
     1501 gttgaggaac ctgtaaaacg gaaaggctca ttgaagccgt atattttctg gaggttcatc
     1561 aggcgcggaa ctcatcaagg cgcgggaata aaatcccatt cagacgccgg atagattcaa
     1621 gcaagccaac ttgtcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat agattccgtt
     1681 ttctgagacg acccctatta tggcgaaggt atcattcagg aagcgttgat gaaggattat
     1741 ctctgtaaaa agttattcaa ccggctttcc ggtaccctgg tgatcagagc gcgctgcggc
     1801 aataacatta ctggcctggc atgttgcaat attctttatc cctcgccccg atacagcggt
     1861 cagctgcata ttaaagagct gtatgtttct cagtgcgatc gaaataaggg cacgggtaaa
     1921 gcgataatgc gctttatagc ccggcttgcg cttgaacagg aatgccttag ccttagctgg
     1981 aacgctgaaa aatccaaccc cggcgctaac cgtttttatc aggctctggg aggcagaata
     2041 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     2101 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     2161 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     2221 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa
     2281 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     2341 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     2401 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     2461 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     2521 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     2581 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     2641 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     2701 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttctcatgtt
     2761 tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc
     2821 agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     2881 ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     2941 gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     3001 caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     3061 ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     3121 ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     3181 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     3241 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     3301 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     3361 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     3421 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     3481 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     3541 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     3601 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     3661 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     3721 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     3781 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     3841 cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc
     3901 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     3961 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     4021 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccgcag acccgccata
     4081 aaacgccctg agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga
     4141 atccataaaa ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca
     4201 gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg
     4261 aatattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
     4321 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
     4381 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
     4441 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
     4501 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
     4561 acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa
     4621 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
     4681 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
     4741 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
     4801 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
     4861 tggtgtatta gctaaagcaa ccagagagct gattacgaga actgtggaaa tcaggaatcc
     4921 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
     4981 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
     5041 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
     5101 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
     5161 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
     5221 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
     5281 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
     5341 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
     5401 aacaaccatt acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc
     5461 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
     5521 taagtatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
     5581 gaacatactg gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg
     5641 aagcatcaag actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa
     5701 aactgtccat atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
     5761 gagtttttgg tgcattcaaa gctgttcacc atgaacagat cgacaatgta acagatgaac
     5821 agcatgtaac acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa
     5881 ttgaacacct gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg
     5941 cgatgctgct tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg
     6001 ggaaaaaatc atggcaattc tggaagaaat agcgctttca gccggcaaac ctgaagccgg
     6061 atctgcgatt ctgataacaa actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac
     6121 ccgtactttt ggacgttccg gcggtttttt gtggcgagtg gtgttcgggc ggtgcgcgca
     6181 agatccatta tgttaaacgg gcgagtttac atctcaaaac cgcccgctta acaccatcag
     6241 aaatcctcag cgcgatttta agcaccaacc cccccccgta acacccaaat ccatactgaa
     6301 agtggctttg ttgaataaat cagatttcgg gtaagtctcc cccgtagcgg gttgtgtttt
     6361 caggcaatac gcacgctttc aggcatacct gctttcgtca ttttgttcag cgctcgtacc
     6421 agggccatag cctccgcaac ctgaccatcg tagtcacgca gcgtcagtga acccccgaac
     6481 agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa tcaataaagc
     6541 cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca gtcgtgatgg
     6601 caaggtcaga atagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg
     6661 cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt
     6721 tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt
     6781 cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc
     6841 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     6901 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     6961 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     7021 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     7081 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     7141 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     7201 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
     7261 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     7321 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     7381 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     7441 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cat
//
