LOCUS       62056_14065        6209 bp DNA     circular SYN 16-DEC-2019
DEFINITION  Cloning vector pLG173, complete sequence.
ACCESSION   MN443778
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Gonzalez LM, Mukhitov N, Voigt CA.
  TITLE     Resilient living materials built by printing bacterial spores
  JOURNAL   Nat. Chem. Biol. (2019) In press
  PUBMED    31792444
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Mukhitov N, Gonzalez LM, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Biological Engineering, MIT, NE47-140, 500 
            Tech Square, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 6209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Biological Engineering, MIT, NE47-140, 500 Tech 
            Square, Cambridge, MA 02139, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label=AmpR promoter
     CDS             211..1068
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2963..4139
                     /label=similar to AmyE
                     /note="similar to AmyE"
     gene            complement(4264..5046)
                     /gene="specR"
                     /label=specR
     terminator      5342..5428
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5447..5474
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_feature    5562..6209
                     /label=similar to AmyE
                     /note="similar to AmyE"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtcg cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct
     1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg
     1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat
     1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc
     2041 agctggattt cactgatgag aatatcgtcg gagataaata taataattcc acggactata
     2101 gactatacta gtatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
     2161 aacgaggatt gttacgtacg ctaatggcgt caaaacaaag actctagacc taggccttaa
     2221 gatctgatca tatgcatccg cgggcccggg ttaacgcgta atccatggat caagagacag
     2281 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggtgccct
     2341 gaatgaactg cagaaagagc tggtagttgg cgcactgttc gaagaactgc cgatgtccag
     2401 taagattctt actatgctgg ttgaaccgga tgctggtaaa gctacttggg ttgctgcttc
     2461 tacttatggt accgatacaa ctactggtga ggaagttaaa ggagctctta aagaaatcca
     2521 cttcagtagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     2581 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggtataaatc
     2641 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     2701 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     2761 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     2821 gcgactaaga aaatgccgtc aaatccgctc gccatgactt cactaacgat gcctttgaaa
     2881 atcttcaagt tcttttctac taattcaagg cgtgtctcac caggtttttg gtttgctccg
     2941 gcgcaaatgc agacaatatc agcatccttg cagggtatgt ttctctttga tgtctttttg
     3001 tttgtgaagt atttcacatt tatattgtgc aacacttcac aaacttttgc aagagaaaag
     3061 ttttgtctga tttatgaaca aaaaagaaac catcattgat ggtttctttc ggtaagtccc
     3121 gtctagcctt gccctcaatg gggaagagaa ccgcttaagc ccgagtcatt atataaacca
     3181 tttagcacgt aatcaaagcc aggctgattc tgaccgggca cttgggcgct gccattatta
     3241 aaaatcactt ttgcgttggt tgtatccgtg tccgcaggca gcgtcagcgt gtaaattccg
     3301 tctgcatttt tagtcattgg ttttccaggc caagatccgg tcaattcaat tactcggctc
     3361 ccatcatgtt tatagatata agcatttacc tggctccaat gattcggatt ttgatagccg
     3421 atggttttgg ccgacgctgg atctctttta acaaaactgt atttctcggt cctcgttaca
     3481 ccatcactgt tcgttccttt taacatgatg gtgtatgttt tgccaaattg gatctccttt
     3541 tccgattgtg aattgatctc catccttaaa cgcctgtcgt ctggtccatt attgatttga
     3601 taaacggctt ttgttgtatt cgcatctgca cgcaaggtaa tcgtcagttg atcattgaaa
     3661 gaatgtgtta cacctgtttt gtaattctca aggaaaacat gaggcgcttt tgcaatatca
     3721 tcaggataaa gcacagctac agacctggca ttgatcgtgc ctgtcagttt accatcgttc
     3781 acttgaaatg aacccgctcc agctttattg tcatacctgc catcaggcaa ttttgttgcc
     3841 gtattgatag agacagagga tgaacctgca tttgccagca caacgccatg tgagccgcgc
     3901 tgattcataa atatctggtt gtttccattc gggttcgaga gttcctcagg ctgtccagcc
     3961 atcacattgt gaaatctatt gaccgcagtg atagcctgat cttcaaataa agcactcccg
     4021 cgatcgccta tttggctttt ccccgggaac ctcacaccat ttccgcctcc ctcaggtctg
     4081 gaaaagaaaa gaggcgtact gcctgaacga gaagctatca ccgcccagcc taaacggatt
     4141 ccccctatgc aagggtttat tgttttctaa aatctgatta ccaattagaa tgaatatttc
     4201 ccaaatatta aataataaaa caaaaaaatt gaaaaaagtg tttccaccat tttttcaatt
     4261 tttttataat ttttttaatc tgttatttaa atagtttata gttaaattta cattttcatt
     4321 agtccattca atattctctc caagataact acgaactgct aacaaaattc tctccctatg
     4381 ttctaatgga gaagattcag ccactgcatt tcccgcaata tcttttggta tgattttacc
     4441 cgtgtccata gttaaaatca tacggcataa agttaatata gagttggttt catcatcctg
     4501 ataattatct attaattcct ctgacgaatc cataatggct cttctcacat cagaaaatgg
     4561 aatatcaggt agtaattcct ctaagtcata atttccgtat attcttttat tttttcgttt
     4621 tgcttggtaa agcattatgg ttaaatctga atttaattcc ttctgaggaa tgtatccttg
     4681 ttcataaagc tcttgtaacc attctccata aataaattct tgtttgggag gatgattcca
     4741 cggtaccatt tcttgctgaa taataattgt taattcaata tatcgtaagt tgcttttatc
     4801 tcctattttt tttgaaatag gtctaatttt ttgtataagt atttctttac tttgatctgt
     4861 caatggttca gatacgacga ctaaaaagtc aagatcacta tttggtttta gtccactctc
     4921 aactcctgat ccaaacatgt aagtaccaat aaggttattt tttaaatgtt tccgaagtat
     4981 ttttttcact ttattaattt gttcgtatgt attcaaatat atcctcctca ctattttgat
     5041 tagtacctat tttatatcca tagttgttaa ttaaataaac ttaatttagt ttatttatag
     5101 atttcattgg cttctaaatt ttttatctag ataataatta ttttagttaa ttttattcta
     5161 gattatatat gatatgatct ttcatttcca taaaactaaa gtaagtgtaa acctattcat
     5221 tgttttaaaa atatctcttg ccagtcacgt tacgttatta gttatagtta ttataacatg
     5281 tattcacgaa cgaaaatcgc cattcgccag ctgcaggaat tcgacctcta gcttgaggca
     5341 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     5401 ggtgaacgct ctcctgagta ggacaaatcc gccgctctag ctaagcagaa ggccatcctg
     5461 acggatggcc tttttgcgtt tctacaaact cttgttaact ctagagctgc cataataacc
     5521 gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttcgatca
     5581 gaccagtttt taatttgtgt gtttccatgt gtccagtttg gaatactctt aacctcattg
     5641 gaaatcgcgg cataatcact ggtggtatga ttgatgaccg cgtcaacaat gacctttatg
     5701 ccatattctt cagcggctgc acacatttct ttaaattctt gttcagtacc taagtaacgg
     5761 ttgccaattt gatacgatgt cggctgatac agccagtacc agttcgacat gcttttatct
     5821 ccttgattcc cttcctttac ttggttaatc ggagatgtct gaatggctgt atatcctgca
     5881 tcatgaatat ccttcatatt gtgttttaac gtattgaacg accaattcca tgcatgaaga
     5941 atggttccgc ttttgatcga cggtgctgta agctcattcg atttgttcgc cgtttcagca
     6001 ctcgcagccg ccggtcctgc cagaaccaaa tgaaacagca ataaaaatcc agcgaataac
     6061 ggcagtaaag aggttttgaa tcgttttgca aacattcttg acactcctta tttgattttt
     6121 tgaagactta cttcggagtc aaaaatccct cttacttcat tcttccgctt cctcctttca
     6181 aaccgatgtg aagactggag aattttgtt
//
