LOCUS       40924_28172       12877 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLG107, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Bruckbauer ST, Kvitko BH, Gallagher L, Karkhoff-Schweizer RR, 
            Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Bruckbauer ST, Kvitko BH, Gallagher L, Karkhoff-Schweizer RR, 
            Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 12877)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12877)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(596..964)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(997..1106)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2188..3045)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3046..3150)
                     /label=AmpR promoter
     CDS             complement(3200..4627)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     IPISSEGSEAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    4782..4800
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     misc_recomb     complement(4809..4856)
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(4809..4856)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(4926..5044)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             5190..6023
                     /codon_start=1
                     /label=rhaS
                     /note="positive regulator of the rhaB promoter"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHGVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             6100..6945
                     /codon_start=1
                     /label=rhaR
                     /note="transcriptional activator of rhaR and rhaS"
                     /translation="VAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQSIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     rep_origin      complement(7111..7699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7848..8639)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9073..12144)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     protein_bind    complement(12258..12305)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(12308..12326)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     protein_bind    12413..12429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(12464..12852)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gatccagccg accaggcttt ccacgcccgc gtgccgctcc atgtcgttcg cgcggttctc
       61 ggaaacgcgc tgccgcgttt cgtgattgtc acgctcaagc ccgtagtccc gttcgagcgt
      121 cgcgcagagg tcagcgaggg cgcggtaggc ccgatacggc tcatggatgg tgtttcgggt
      181 cgggtgaatc ttgttgatgg cgatatggat gtgcaggttg tcggtgtcgt gatgcacggc
      241 actgacgcgc tgatgctcgg cgaagccaag cccagcgcag atgcggtcct caatcgcgcg
      301 caacgtctcc gcgtcgggct tctctcccgc gcggaagcta accagcaggt gataggtctt
      361 gtcggcctcg gaacgggtgt tgccgtgctg ggtcgccatc acctcggcca tgacagcggg
      421 cagggtgttt gcctcgcagt tcgtgacgcg cacgtgaccc aggcgctcgg tcttgccttg
      481 ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg
      541 gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct
      601 ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga
      661 tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg
      721 tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct
      781 cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca
      841 ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt
      901 ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
      961 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
     1021 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     1081 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     1141 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     1201 tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc cctgctgttt
     1261 tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac tgaggggaca
     1321 ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt gaatcccgcg
     1381 cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg agggcggatg
     1441 tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac atgcgggaaa
     1501 cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg cacatcaagg
     1561 ccaagcccgc cgatgtgccc gcaccgcagg ccaaggctgc ggaacccgcg ccggcaccca
     1621 agacgccgga gccacggcgg ccgaagcagg ggggcaaggc tgaaaagccg gcccccgctg
     1681 cggccccgac cggcttcacc ttcaacccaa caccggacaa aaaggatcct ctacgccgga
     1741 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     1801 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     1861 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gctgcctcgc
     1921 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     1981 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2041 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     2101 aactatgcgg catcagagca gattgtactg agagtgcacc ataaaatcaa tctaaagtat
     2161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     2521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     2821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcaga
     3181 tctgcaggtc gaccgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     3241 agccatccag tttactttgc agggcttccc aaccttccca gagggcgccc cagctggcaa
     3301 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     3361 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     3421 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct
     3481 ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctttctctg agctgtaaca
     3541 gcctgaccgc aacaaacgag aggatcgaga ccatccgctc cagattatcc ggctcctcca
     3601 tgcgttgcct ctcggctcct gctccggttt tccatgcctt atggaactcc tcgatccgcc
     3661 agcgatgggt ataaatgtcg atgacgcgca aggcttgggc tagcgactcg accggttcgc
     3721 tggtcagcaa caaccatttc aacggggtct cacccttggg cgggttaatc tcctcggcca
     3781 gcaccgcgtt gagcgtgata ttcccctgtt ttagcgtgat gcgcccactg cgcaggctca
     3841 agctcgcctt gcgggctggt cgatttttac gtttaccgcg tttatccacc acgccctttt
     3901 gcggaatgct gatctgatag ccacccaact ccggttggtt cttcagatgg tcgtacagat
     3961 acaacccaga ctctacgtcc ttgcgtgggt gcttggagcg caccacgaag cgctcgttat
     4021 gcgccagttt gtcctgcaga taagcatgaa tatcggcttc gcggtcacag accgcaatca
     4081 cgttgctcat catgctgccc atgcgtaacc ggctagttgc ggccgctgcc agccatttgc
     4141 cactctcctt ttcatccgca tcggcagggt catccgggcg catccaccac tcctgatgca
     4201 gtaatcctac ggtgcggaat gtggtggcct cgagcaagag aacggagtga acccaccatc
     4261 cgcgggattt atcctgaata gagcccagct tgccaagctc ttcggcgacc tggtggcgat
     4321 aactcaaaga ggtggtgtcc tcaatggcca gcagttcggg aaactcctga gccaacttga
     4381 ctgtttgcat ggcgccagcc tttctgatcg cctcggcaga aacgttggga ttgcggataa
     4441 atcggtaagc gccttcctgg gcggcttcac taccctctga tgagatgggt attgatttac
     4501 cagaatattt tgccaattgg gcggcgacgt taaccaagcg ggcagtacgg cgaggatcac
     4561 ccagcgccgc cgaagagaac acagatttag cccagtcggc cgcacgatga agagcagaag
     4621 ttatcatgaa cgttaccatg ttaggaggtc acatggaaga tcagatcctg gaaaacggga
     4681 aaggttccgt tcaggacgct acttgtgtac gggatcggtc gacggatccc aagcttcttc
     4741 tagtaacggc cgccagtgtg ctggaattcg cccttgaatt cctgtctctt atacacatct
     4801 cgccgacgga agttcctata ctttctagag aataggaact tcggaatagg aacttcattt
     4861 aaatggcgcg ccgctaggcc ttacttgtac agctcgtcca catatgtgat cctgctgaat
     4921 ttcattacga ccagtctaaa aagcgcctga attcgcgacc ttctcgttac tgacaggaaa
     4981 atgggccatt ggcaaccagg gaaagatgaa cgtgatgatg ttcacaattt gctgaattgt
     5041 ggtgatgtga tgctcaccgc atttcctgaa aattcacgct gtatcttgaa aaatcgacgt
     5101 tttttacgtg gttttccgtc gaaaatttaa ggtaagaacc tgacctcgtg attactattt
     5161 cgccgtgttg acgacatcag gaagccagta tgaccgtatt acatagtgtg gatttttttc
     5221 cgtctggtaa cgcgtccgtg gcgatagaac cccggctccc gcaggcggat tttcctgaac
     5281 atcatcatga ttttcatgaa attgtgattg tcgaacatgg cacgggtatt catgtgttta
     5341 atgggcagcc ctataccatc accggtggca cggtctgttt cgtacgcgat catgatcggc
     5401 atctgtatga acataccgat aatctgtgtc tgaccaatgt gctgtatcgc tcgccggatc
     5461 gatttcagtt tctcgccggg ctgaatcagt tgctgccaca agagctggat gggcagtatc
     5521 cgtctcactg gcgcgttaac cacggcgtat tgcagcaggt gcgacagctg gttgcacaga
     5581 tggaacagca ggaaggggaa aatgatttac cctcgaccgc cagtcgcgag atcctgttta
     5641 tgcaattact gctcttgctg cgtaaaagca gtttgcagga gaacctggaa aacagcgcat
     5701 cacgtctcaa cttgcttctg gcctggctgg aggaccattt tgccgatgag gtgaattggg
     5761 atgccgtggc ggatcaattt tctctttcac tgcgtacgct acatcggcag cttaagcagc
     5821 aaacgggact gacgcctcag cgatacctga accgcctgcg actgatgaaa gcccgacatc
     5881 tgctacgcca cagcgaggcc agcgttactg acatcgccta tcgctgtgga ttcagcgaca
     5941 gtaaccactt ttcgacgctt tttcgccgag agtttaactg gtcaccgcgt gatattcgcc
     6001 agggacggga tggctttctg caataacgcg aatcttctca acgtatttgt acgccatatt
     6061 gcgaataatc aacttcgttc tctggccgag gtagccacgg tggcgcatca gttaaaactt
     6121 ctcaaagatg atttttttgc cagcgaccag caggcagtcg ctgtggctga ccgttatccg
     6181 caagatgtct ttgctgaaca tacacatgat ttttgtgagc tggtgattgt ctggcgcggt
     6241 aatggcctgc atgtactcaa cgatcgccct tatcgcatta cccgtggcga tctcttttac
     6301 attcatgctg acgataaaca ctcctacgct tccgttaacg atctggtttt gcagagtatt
     6361 atttattgcc cggagcgtct gaagctgaat cttgactggc agggggcgat tccgggattt
     6421 aacgccagcg cagggcaacc acactggcgc ttaggtagca tggggatggc gcaggcgcgg
     6481 caggttatcg gtcagcttga gcatgaaagt agtcagcatg tgccgtttgc taacgaaatg
     6541 gctgagttgc tgttcgggca gttggtgatg ttgctgaatc gccatcgtta caccagtgat
     6601 tcgttgccgc caacatccag cgaaacgttg ctggataagc tgattacccg gctggcggct
     6661 agcctgaaaa gtccctttgc gctggataaa ttttgtgatg aggcatcgtg cagtgagcgc
     6721 gttttgcgtc agcaatttcg ccagcagact ggaatgacca tcaatcaata tctgcgacag
     6781 gtcagagtgt gtcatgcgca atatcttctc cagcatagcc gcctgttaat cagtgatatt
     6841 tcgaccgaat gtggctttga agatagtaac tatttttcgg tggtgtttac ccgggaaacc
     6901 gggatgacgc ccagccagtg gcgtcatctc aattcgcaga aagattaaat gcatttggta
     6961 acgaatcaga caattgacgg cttgacggag tagcataggg tttgcagaat ccctgcttcg
     7021 tccatttgac aggcaggtac caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7141 tcacaaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7321 ggtatctcag ttcggtgtag gccgttcgct ccaagctggg ctgtgtgcac gaacccccgt
     7381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagag gaacagtatt
     7561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7621 cggcaaacaa gccaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7741 gaacgaaatc gatgagctcg cacgaaccca gttgacataa gcctgttcgg ttcgtccgga
     7801 ctagtgatgg aaatgttgga tcctcatact cttccttttt caattcagaa gaactcgtca
     7861 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     7921 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     7981 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     8041 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     8101 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     8161 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     8221 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     8281 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgggatga caggagatcc
     8341 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     8401 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     8461 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     8521 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     8581 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     8641 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     8701 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     8761 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     8821 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     8881 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg
     8941 ttccgcttcc tttagcagcc cttgcgccat gcatttgtta atcgaattat ccattgctgt
     9001 tgacaaaggg aatcagggga tccggactag tacgcgaaat acgggcagac atggcctgcc
     9061 cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg cgctcagctg
     9121 taattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca tatggaaacc
     9181 gtcgatattc agccatgtgc cttcttccgc gtgcagcaga tggcgatggc tggtttccat
     9241 cagttgctgt tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg
     9301 gccataattc aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga agacgtacgg
     9361 ggtatacatg tctgacaatg gcagatccca gcggtcaaaa caggcggcag taaggcggtc
     9421 gggatagttt tcttgcggcc ctaatccgag ccagtttacc cgctctgcta cctgcgccag
     9481 ctggcagttc aggccaatcc gcgccggatg cggtgtatcg ctcgccactt caacatcaac
     9541 ggtaatcgcc atttgaccac taccatcaat ccggtaggtt ttccggctga taaataaggt
     9601 tttcccctga tgctgccacg cgtgagcggt cgtaatcagc accgcatcag caagtgtatc
     9661 tgccgtgcac tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc
     9721 gacccaggcg ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg
     9781 tgcacgggtg aactgatcgc gcagcggcgt cagcagttgt tttttatcgc caatccacat
     9841 ctgtgaaaga aagcctgact ggcggttaaa ttgccaacgc ttattaccca gctcgatgca
     9901 aaaatccatt tcgctggtgg tcagatgcgg gatggcgtgg gacgcggcgg ggagcgtcac
     9961 actgaggttt tccgccagac gccactgctg ccaggcgctg atgtgcccgg cttctgacca
    10021 tgcggtcgcg ttcggttgca ctacgcgtac tgtgagccag agttgcccgg cgctctccgg
    10081 ctgcggtagt tcaggcagtt caatcaactg tttaccttgt ggagcgacat ccagaggcac
    10141 ttcaccgctt gccagcggct taccatccag cgccaccatc cagtgcagga gctcgttatc
    10201 gctatgacgg aacaggtatt cgctggtcac ttcgatggtt tgcccggata aacggaactg
    10261 gaaaaactgc tgctggtgtt ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa
    10321 gaccagaccg ttcatacaga actggcgatc gttcggcgta tcgccaaaat caccgccgta
    10381 agccgaccac gggttgccgt tttcatcata tttaatcagc gactgatcca cccagtccca
    10441 gacgaagccg ccctgtaaac ggggatactg acgaaacgcc tgccagtatt tagcgaaacc
    10501 gccaagactg ttacccatcg cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc
    10561 aggtagcgaa agccattttt tgatggacca tttcggcaca gccgggaagg gctggtcttc
    10621 atccacgcgc gcgtacatcg ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc
    10681 ttcatactgc accgggcggg aaggatcgac agatttgatc cagcgataca gcgcgtcgtg
    10741 attagcgccg tggcctgatt cattccccag cgaccagatg atcacactcg ggtgattacg
    10801 atcgcgctgc accattcgcg ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc
    10861 ggtcagacga ttcattggca ccatgccgtg ggtttcaata ttggcttcat ccaccacata
    10921 caggccgtag cggtcgcaca gcgtgtacca cagcggatgg ttcggataat gcgaacagcg
    10981 cacggcgtta aagttgttct gcttcatcag caggatatcc tgcaccatcg tctgctcatc
    11041 catgacctga ccatgcagag gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg
    11101 cttgccgttc agcagcagca gaccattttc aatccgcacc tcgcggaaac cgacatcgca
    11161 ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag
    11221 attcgggatt tcggcgctcc acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg
    11281 atcggcataa ccaccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc
    11341 gacctgcgtt tcaccctgcc ataaagaaac tgttacccgt aggtagtcac gcaactcgcc
    11401 gcacatctga acttcagcct ccagtacagc gcggctgaaa tcatcattaa agcgagtggc
    11461 aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat
    11521 gccgctcatc cgccacatat cctgatcttc cagataactg ccgtcactcc agcgcagcac
    11581 catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg
    11641 caaacgactg tcctggccgt aaccgaccca gcgcccgttg caccacagat gaaacgccga
    11701 gttaacgcca tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt catcaacatt
    11761 aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga acaaacggcg gattgaccgt
    11821 aatgggatag gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga
    11881 ggggacgacg acagtatcgg cctcaggaag atcgcactcc agccagcttt ccggcaccgc
    11941 ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga
    12001 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12061 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12121 cagtgaatcc gtaatcatgg tcatagctgt ttcctgtgtg ataaagaaag ttaaaatgcc
    12181 gccagcggaa ctggcggctg tgggattaac tgcgcgtcgc cgctttcatc ggttgtcgga
    12241 tccccctagg gcgcgccgaa gttcctatac tttctagaga ataggaactt cggaatagga
    12301 acttcttaga tgtgtataag agacagctcg agaagggcga attctgcagg tcgactctag
    12361 aggatccccg ggtaccgcat gcgatatcga gctctcccgg gaattccaca aattgttatc
    12421 cgctcacaat tccacatgtg gaattccaca tgtggaattc ccatgtcagc cgttaagtgt
    12481 tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc
    12541 tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt
    12601 tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat
    12661 tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag
    12721 cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca
    12781 tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct
    12841 gatcttcaga tcctctacgc cggacgcatc gtggccg
//
