LOCUS       40924_27667        5693 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLEMY, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5693)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             352..381
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             385..414
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             418..447
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             451..480
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             484..513
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             517..546
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             550..579
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             583..612
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             616..645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             649..678
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             682..711
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             715..744
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      787..1279
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     promoter        1526..1931
                     /label=LEU2 promoter
     CDS             1932..3023
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      3027..3503
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     promoter        complement(3582..3600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3858..4446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4620..5477)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5478..5582)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttctcga gccccggatc cgccagcatg
      301 caagggctgc aggcttgcct tcaggtcgtc gattccgagg aggaccttac tgagcaaaag
      361 ctcatttccg aggaggacct tactgagcaa aagctcattt ccgaggagga ccttactgag
      421 caaaagctca tttccgagga ggaccttact gagcaaaagc tcatttccga ggaggacctt
      481 actgagcaaa agctcatttc cgaggaggac cttactgagc aaaagctcat ttccgaggag
      541 gaccttactg agcaaaagct catttccgag gaggacctta ctgagcaaaa gctcatttcc
      601 gaggaggacc ttactgagca aaagctcatt tccgaggagg accttactga gcaaaagctc
      661 atttccgagg aggaccttac tgagcaaaag ctcatttccg aggaggacct tactgagcaa
      721 aagctcattt ccgaggagga ccttactgag caaaagcaat ctcttgaggc tcccctgtaa
      781 tctagagaaa atccttgctt aaaaagattc tcttttttta tgatatttgt acataaactt
      841 tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg
      901 tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatgt
      961 aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg
     1021 cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag gtgtaacaga
     1081 aaatcatgaa actatgattc ctaatttata tattggagga ttttctctaa aaaaaaaaaa
     1141 atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa gtcaatacct
     1201 tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct gtcagaaacg
     1261 gccttaacga cgtagtcgaa gatcttcgag gagaacttct agtatatcta catacctaat
     1321 attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa
     1381 aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata
     1441 attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga
     1501 ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag
     1561 agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc
     1621 gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct
     1681 gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga
     1741 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
     1801 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
     1861 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tttcaaggat
     1921 ataccattct aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc
     1981 aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg
     2041 tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc
     2101 cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg
     2161 tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc
     2221 gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt
     2281 tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag
     2341 aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt
     2401 gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca
     2461 tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg
     2521 cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat
     2581 tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc
     2641 acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct
     2701 ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca
     2761 agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga
     2821 ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga
     2881 acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta
     2941 tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg
     3001 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
     3061 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat
     3121 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     3181 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
     3241 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
     3301 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
     3361 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     3421 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     3481 aacggcctta acgacgtagt cgagtcgacc cccccgagct cgaattcatc gatgatatca
     3541 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     3601 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3661 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3781 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4021 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     4081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4261 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4741 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4921 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5221 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5581 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5641 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tga
//
