LOCUS       40924_27663        5003 bp DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(104..949)
                     /label=rhaR
                     /note="transcriptional activator of rhaR and rhaS"
     CDS             complement(1026..1859)
                     /label=rhaS
                     /note="positive regulator of the rhaB promoter"
     promoter        2005..2123
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             2147..2599
                     /label=T7 lysozyme
                     /note="lysozyme from bacteriophage T7"
     terminator      2672..2719
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2943..3599)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3600..3702)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(4228..4773)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(4964..4985)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
       61 gggagaggcg gtttgcgtat tgggcgcccg ataagcttaa ttaatctttc tgcgaattga
      121 gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt
      181 tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga
      241 gaagatattg cgcatgacac actctgacct gtcgcagata ttgattgatg gtcattccag
      301 tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt
      361 tatccagcgc aaagggactt ttcaggctag ccgccagccg ggtaatcagc ttatccagca
      421 acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca
      481 tcaccaactg cccgaacagc aactcagcca tttcgttagc aaacggcaca tgctgactac
      541 tttcatgctc aagctgacca ataacctgcc gcgcctgcgc catccccatg ctacctaagc
      601 gccagtgtgg ttgccctgcg ctggcgttaa atcccggaat cgccccctgc cagtcaagat
      661 tcagcttcag acgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag
      721 cgtaggagtg tttatcatca gcatgaatgt aaaagagatc gccacgggta atgcgataag
      781 ggcgatcgtt gagtacatgc aggccattac cgcgccagac aatcaccagc tcacaaaaat
      841 catgtgtatg ttcagcaaag acatcttgcg gataacggtc agccacagcg actgcctgct
      901 ggtcgctggc aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct
      961 cggccagaga acgaagttga ttattcgcaa tatggcgtac aaatacgttg agaagattcg
     1021 cgttattgca gaaagccatc ccgtccctgg cgaatatcac gcggtgacca gttaaactct
     1081 cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata ggcgatgtca
     1141 gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc
     1201 aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt
     1261 gaaagagaaa attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc
     1321 agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg
     1381 cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt
     1441 aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat
     1501 acgctgtggt taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac
     1561 tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attggtcaga
     1621 cacagattat cggtatgttc atacagatgc cgatcatgat cgcgtacgaa acagaccgtg
     1681 ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca
     1741 atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt
     1801 tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata
     1861 ctggcctcct gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt
     1921 aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt
     1981 caggaaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt
     2041 catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc
     2101 aggcgctttt tagactggtc gtaatgaaat tcaggaggtg gtcgacatgg ctcgtgtaca
     2161 gtttaaacaa cgtgaatcta ctgacgcaat ctttgttcac tgctcggcta ccaagccaag
     2221 tcagaatgtt ggtgtccgtg agattcgcca gtggcacaaa gagcagggtt ggctcgatgt
     2281 gggataccac tttatcatca agcgagacgg tactgtggag gcaggacgag atgagatggc
     2341 tgtaggctct cacgctaagg gttacaacca caactctatc ggcgtctgcc ttgttggtgg
     2401 tatcgacgat aaaggtaagt tcgacgctaa ctttacgcca gcccaaatgc aatcccttcg
     2461 ctcactgctt gtcacactgc tggctaagta cgaaggcgct gtgcttcgcg cccatcatga
     2521 ggtggcgccg tacgcttgcc cttcgttcga ccttaagcgt tggtgggaga agaacgaact
     2581 ggtcacttct gaccgtggat aaggatcccc gcgccctcat ccgaaagggc gtattcatat
     2641 gccctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     2701 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
     2761 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
     2821 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
     2881 tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat
     2941 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     3001 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     3061 ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc catattggcc
     3121 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     3181 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     3241 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     3301 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     3361 ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc aagaatgtga
     3421 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     3481 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     3541 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     3601 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3661 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     3721 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     3781 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     3841 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
     3901 tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca
     3961 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     4021 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     4081 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     4141 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     4201 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     4261 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     4321 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     4381 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     4441 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     4501 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     4561 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     4621 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     4681 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     4741 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     4801 tatttctagg tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     4861 cgatataagt tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg
     4921 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     4981 cattaattgc gttgcgctca ctg
//
