LOCUS       62056_13790        6192 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    531..611
                     /label=MCS
                     /note="multiple cloning site"
     CDS             615..638
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     LTR             718..1311
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    1522..1662
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1848..2436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2610..3467)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3468..3572)
                     /label=AmpR promoter
     LTR             3818..4405
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    4468..4667
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             4868..5284
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     CDS             5328..6119
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        6140..6192
                     /label=CMV
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      601 gatccaccgg tatggactac aaggacgacg atgacaagta agcggccgcg actctagcct
      661 taatggccta acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
      721 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
      781 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
      841 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
      901 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
      961 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     1021 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     1081 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     1141 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     1201 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     1261 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
     1321 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     1381 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1441 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1501 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     1561 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     1621 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     1681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2281 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2521 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2581 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2641 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2701 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2761 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2821 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2881 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     2941 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3001 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3061 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3121 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3181 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     3241 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3301 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3361 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3421 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3481 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3601 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3661 tttcgtcttc aagaattcat accagatcac cgaaaactgt cctccaaatg tgtccccctc
     3721 acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc cccacactca
     3781 ccggagccaa agccgcggcc cttccgtttc tttgcttttg aaagacccca cccgtaggtg
     3841 gcaagctagc ttaagtaacg ccactttgca aggcatggaa aaatacataa ctgagaatag
     3901 aaaagttcag atcaaggtca ggaacaaaga aacagctgaa taccaaacag gatatctgtg
     3961 gtaagcggtt cctgccccgg ctcagggcca agaacagatg agacagctga gtgatgggcc
     4021 aaacaggata tctgtggtaa gcagttcctg ccccggctcg gggccaagaa cagatggtcc
     4081 ccagatgcgg tccagccctc agcagtttct agtgaatcat cagatgtttc cagggtgccc
     4141 caaggacctg aaaatgaccc tgtaccttat ttgaactaac caatcagttc gcttctcgct
     4201 tctgttcgcg cgcttccgct ctccgagctc aataaaagag cccacaaccc ctcactcggc
     4261 gcgccagtct tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg
     4321 ctgtttgcat ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     4381 ctacccacga cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca
     4441 gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
     4501 ttgtctagtg tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct
     4561 ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg
     4621 gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat
     4681 gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt
     4741 cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct
     4801 gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga
     4861 aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
     4921 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     4981 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     5041 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     5101 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     5161 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     5221 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     5281 cgccggaatt ccgatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5341 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5401 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5461 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5521 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5581 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5641 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5701 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5761 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5821 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5881 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5941 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6001 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6061 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6121 gagcgggact ctggggttcg aatagtaatc aattacgggg tcattagttc atagcccata
     6181 tatggagttc cg
//
