LOCUS       40924_27653        5958 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLECL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5958)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             302..1012
                     /label=mECFP
                     /note="enhanced CFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_feature    1013..1042
                     /label=1xMYC tag
                     /note="1xMYC tag"
     CDS             1013..1042
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      1052..1544
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     promoter        1791..2196
                     /label=LEU2 promoter
     CDS             2197..3288
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      3292..3768
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     promoter        complement(3847..3865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4885..5742)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5743..5847)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttctcga gccccggatc cgccagcatg
      301 cagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      361 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      421 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      481 cactactttg acttggggtg ttcaatgctt ttcaagatac ccagatcata tgaaacggca
      541 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      601 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      661 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      721 ggaatacaac tatatttcac acaatgtata catcactgca gacaaacaaa agaatggaat
      781 caaagctcat ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      841 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      901 gtccacacaa tctaagcttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
      961 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aagagcaaaa
     1021 gctcatttcc gaggaggacc tttaatctag agaaaatcct tgcttaaaaa gattctcttt
     1081 ttttatgata tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat
     1141 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
     1201 tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc
     1261 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac
     1321 cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg
     1381 gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca
     1441 tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct
     1501 caagaatttt actctgtcag aaacggcctt aacgacgtag tcgaagatct tcgaggagaa
     1561 cttctagtat atctacatac ctaatattat tgccttatta aaaatggaat cccaacaatt
     1621 acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt
     1681 tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta attggttgtt
     1741 tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga
     1801 atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct
     1861 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat
     1921 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag
     1981 aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg
     2041 actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt
     2101 ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag
     2161 caatatatat atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc
     2221 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
     2281 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
     2341 gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag
     2401 gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
     2461 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
     2521 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
     2581 ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
     2641 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
     2701 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
     2761 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
     2821 accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
     2881 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
     2941 ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct
     3001 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
     3061 ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct
     3121 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
     3181 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
     3241 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
     3301 ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
     3361 aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
     3421 tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa
     3481 tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg
     3541 gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat
     3601 attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg
     3661 accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt
     3721 ccctcaagaa ttttactctg tcagaaacgg ccttaacgac gtagtcgagt cgaccccccc
     3781 gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc
     3841 ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat
     3901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5881 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5941 tctcgcgcgt ttcggtga
//
