LOCUS       V005067                12242 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005067
VERSION     V005067
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R.
  TITLE     A load driver device for engineering modularity in biological
            networks
  JOURNAL   Nat. Biotechnol. 32 (12), 1268-1275 (2014)
   PUBMED   25419739
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Mishra D, Rivera-Ortiz PM, Lin A, Del Vecchio D, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2014) Synthetic Biology Center, MIT, 500
            Technology Square, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 12242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2014"; volume: "32"; issue: "12"; pages:
            "1268-1275"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-SEP-2014) Synthetic Biology Center, MIT, 500 Technology Square,
            NE47, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    50..542
                     /label="HO-R"
                     /note="HO-R"
     promoter        569..599
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             653..1309
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     rep_origin      1487..2075
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2383..2866
                     /label="HO-L"
                     /note="HO-L"
     protein_bind    2966..2986
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        3040..3439
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     primer_bind     3443..3459
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    3485..3509
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3512..4516
                     /label="rtTA"
                     /note="tetracycline-controlled transactivator, comprising a
                     fusion of the reverse tetracycline repressor rTetR with the
                     C-terminal activation domain of herpes simplex virus VP16"
     protein_bind    complement(4521..4545)
                     /gene="mutant version of attB"
                     /label="attB2"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(4523..4544)
                     /label="attB2"
                     /note="attB2"
     terminator      4568..4755
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    4858..4879
                     /label="attB4"
                     /note="attB4"
     protein_bind    4859..4879
                     /gene="mutant version of attB"
                     /label="attB4"
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    4933..5203
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      5229..5385
                     /regulatory_class="promoter"
     protein_bind    5395..5419
                     /gene="mutant version of attB"
                     /label="attB1"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5396..5416
                     /label="attP1"
                     /note="attP1"
     CDS             5428..7293
                     /gene="SKN7"
                     /label="Transcription factor SKN7"
                     /note="Transcription factor SKN7 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P38889"
     protein_bind    complement(7301..7325)
                     /gene="mutant version of attB"
                     /label="attB2"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(7303..7324)
                     /label="attB2"
                     /note="attB2"
     terminator      7340..7587
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     misc_feature    7688..7709
                     /label="attB4"
                     /note="attB4"
     protein_bind    7689..7709
                     /gene="mutant version of attB"
                     /label="attB4"
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     regulatory      7743..8475
                     /regulatory_class="promoter"
     misc_feature    8159..8198
                     /label="SKN7 binding site"
                     /note="SKN7 binding site"
     misc_feature    8199..8238
                     /label="SKN7 binding site"
                     /note="SKN7 binding site"
     protein_bind    8487..8511
                     /gene="mutant version of attB"
                     /label="attB1"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8487..8508
                     /label="attP1"
                     /note="attP1"
     CDS             8519..9232
                     /label="GFP"
                     /note="green fluorescent protein"
     protein_bind    complement(9240..9264)
                     /gene="mutant version of attB"
                     /label="attB2"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(9242..9263)
                     /label="attB2"
                     /note="attB2"
     terminator      9274..9471
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    9640..9661
                     /label="attB4"
                     /note="attB4"
     protein_bind    9641..9661
                     /gene="mutant version of attB"
                     /label="attB4"
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     regulatory      9711..10118
                     /regulatory_class="promoter"
     promoter        9716..10114
                     /gene="S. cerevisiae TEF1"
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     primer_bind     10118..10134
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    10160..10184
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10193..10999
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(11004..11028)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      11033..11157
                     /label="PHO5 Terminator"
                     /note="PHO5 Terminator"
                     /regulatory_class="terminator"
     CDS             11314..12120
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gtatcactag tattacagag gtaagttata acagtcgcct aacccctacc tgggggaaca
       61 acttcacaga atgttttgtc atattgtcga agtggtcaca aaacaagaga agttccgcca
      121 attataaaaa gggaacccgt atatttcagc ttcacggatg atttccaggg tgagagtact
      181 gtatatgggc ttacgataga aggccataaa aatttcttgc ttggcaacaa aatagaagtg
      241 aaatcatgtc gaggctgctg tgtgggagaa cagcttaaaa tatcacaaaa aaagaatcta
      301 aaacactgtg ttgcttgtcc cagaaaggga atcaagtatt tttataaaga ttggagtggt
      361 aaaaatcgag tatgtgctag atgctatgga agatacaaat tcagcggtca tcactgtata
      421 aattgcaagt atgtaccaga agcacgtgaa gtgaaaaagg caaaagacaa aggcgaaaaa
      481 ttgggcatta cgcccgaagg tttgccagtt aaaggaccag agtgtaggga taacagggta
      541 atctgaggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      601 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      661 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      721 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      781 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      841 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      901 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      961 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1021 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1081 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1141 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1201 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1261 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatggcctgt
     1321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1621 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2161 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2221 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2281 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2341 cgggcagtga gcgcaacgca acgtattacc ctgttatccc tatgggcacg agtgaaacaa
     2401 agctaaaacc tttatttagc atggccattg aatgtaacaa ttatatatat cgcaagcaca
     2461 aaaaatcaag gagagagaac taccactttg ttcatgtgta caatgttcat tatctccata
     2521 agcaaaaaaa aaaaatagaa aacatatgct ataaggttga tattctcacg agtaagcggc
     2581 acttgctact tattgacatt gcagattttt ggctacagaa atagtatatt agagattata
     2641 attgctaatc aaatcaaaat ataaaattag taaaccaaac catttatacc cttccttagt
     2701 agttatggat tgttttttaa tgatatttct gcaaaccaaa gaaagattgt tatccagata
     2761 gaatttagtt ttgatattca tttttttgtt gaagattgaa cgccatatct gggcctcata
     2821 attcaaaaga cggtgccatt atcggtagcg tttcgcattg tactgggagc gaggtttacc
     2881 gagctcttat tggttttcaa acttcattga ctgtgccgtt taccgagctc ttattggttt
     2941 tcaaacttca ttgactgtgc cgagccaact ttgtatagaa aagttggctc cgaatttctc
     3001 gagcgatact aacgccgcca tccagtgtcg aaaacgagct catagcttca aaatgtttct
     3061 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
     3121 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
     3181 cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa
     3241 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt gatttttttc
     3301 tctttcgatg acctcccatt gatatttaag ttaataaacg gtcttcaatt tctcaagttt
     3361 cagtttcatt tttcttgttc tattacaact ttttttactt cttgctcatt agaaagaaag
     3421 catagcaatc taatctaagt tttctagaac tagtggatcc cccccggacg acagagaatt
     3481 cgacccaagt ttgtacaaaa aagcaggctg aatgtctaga ttagataaaa gtaaagtgat
     3541 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     3601 cgcccagaag cttggtgtag agcagcctac actgtattgg catgtaaaaa ataagcgggc
     3661 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     3721 aaaaggggaa agctggcaag attttttacg caataacgct aaaagtttta gatgtgcttt
     3781 actaagtcat cgcaatggag caaaagtaca ttcagataca cggcctacag aaaaacagta
     3841 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaacgc
     3901 gttatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     3961 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     4021 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     4081 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     4141 ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg atctcccgga
     4201 cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc tccccgcggg
     4261 acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg acgagctcca
     4321 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     4381 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     4441 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     4501 tgacgagtac ggtgggtagt acccagcttt cttgtacaaa gtggtctacc gcgccacttc
     4561 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     4621 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tgttcttgag
     4681 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     4741 ccacacctct accggcagat cctgaggtgc gtttttatgc ttgtagtatt gtataatgtt
     4801 tttaagatcc gtgcgttttt atgcttgtag tattgtataa tgtttttaag atccgagcca
     4861 actttgtata gaaaagttgg ctccgaattt ctcgagctac cggcagatca attcctcgat
     4921 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     4981 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5041 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     5101 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     5161 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
     5221 cggtacccta tggcatgcat gtgctctgta tgtatataaa actcttgttt tcttcttttc
     5281 tctaaatatt ctttccttat acattaggtc ctttgtagca taaattacta tacttctata
     5341 gacacgcaaa cacaaataca cacactaaat taccggatca attcggaatt cgacccaagt
     5401 ttgtacaaaa aagcaggctg aaaaaaaatg agcttttcca ccataaatag caacgtcaat
     5461 aaaaccaccg gcgatagcaa taataacacc accgagaaca gttcgactgc agacctttta
     5521 ggaatggact tgttgcagag cgggcctcga ctgatgaaca cgatgcagcc aaacaactct
     5581 tctgacatgc tgcacattaa caacaagact aataacgttc aacaaccagc tggaaacaca
     5641 aatatcagca gtgctaatgc gggagcaaag gctccagcaa atgagttcgt aagaaaactg
     5701 ttcaggatac tggaaaacaa tgaatatcct gacattgtaa cttggactga gaacggcaaa
     5761 agtttcgtcg ttttggacac aggaaagttc actacgcata tattgcctaa tcacttcaaa
     5821 cattcaaatt ttgcatcttt tgtaaggcaa ctaaacaagt atgactttca caaggttaag
     5881 agaagtcccg aggaaagaca gagatgtaaa tatggcgaac aaagttggga gtttcagcat
     5941 ccagaattta gagtccatta cggaaaaggt ctcgataaca tcaaaaggaa aattccggcg
     6001 caaaggaaag tgcttttgga tgaatctcaa aaggctcttt tgcatttcaa tagtgaaggc
     6061 actaacccca acaatccttc tgggtctctt ttgaatgaat ccaccacaga gctgttgtta
     6121 agcaataccg taagtaaaga tgcatttgga aatctaagaa ggcgagtaga caaactacaa
     6181 aaggagttgg atatgtccaa aatggagagt tatgctacta aagtagaact acaaaagttg
     6241 aactcgaaat acaatacggt tatcgaaagt ttgataacat tcaagaccat aaatgaaaat
     6301 ttactcaaca acttcaacac tctgtgttcc actttggcaa ataatggtat tgaagtgcca
     6361 atatttggcg acaatggaaa ccgtaaccca actggtaata ccaacccagc aacaacaaca
     6421 gctatccaaa gcaacaacaa caccaacaat gcttctccgg caacatctac agtttcctta
     6481 caactaccta atttacccga tcagaatagc ctaacaccaa atgctcaaaa taacacagtc
     6541 acgctacgaa aaggtttcca tgtactgttg gtggaagatg acgcagtgtc tatacagttg
     6601 tgttcaaaat ttttacggaa atatggctgt actgtccaag ttgtcagcga cggtctttca
     6661 gctatctcaa cactagagaa gtataggtat gatttggttt taatggaaat tgttatgcca
     6721 aacctagatg gtgccacagc gacatccatt gtcagaagtt ttgataatga gactcccatc
     6781 attgccatga caggtaacat tatgaatcaa gacttgatca catacttaca acatggaatg
     6841 aatgatatct tggccaaacc attcacgagg gatgatttac actcaatttt aatacgttat
     6901 ctaaaggacc gtattccttt atgcgaacag caattaccac ctcgcaactc ttcgccacaa
     6961 actcattcca acaccaatac tgctaattcg aatcctaata cgattaatga acagtcgtta
     7021 gccatgttac cacaagataa tccgtcaact actacccctg ttaccccagg tgcctctata
     7081 tcttctgcac agcatgttca acaaggtcaa caagaacagc agcatcaaat tttccatgct
     7141 cagcagcagc agcagcatca caacgccatt gctaatgcta ggtcagacgt agccataccg
     7201 aatttggaac atgaaatcaa cactgtacca cattcctcaa tgggttccac tccgcaatta
     7261 ccacaatcta cacttcaaga aaaccagcta tcataataat acccagcttt cttgtacaaa
     7321 gtggtctacg agggccgcat catgtaatta gttatgtcac gcttacattc acgccctccc
     7381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     7441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     7501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     7561 ttgggacgct cgaaggcttt aatttgcggc cctgaggtct aatacccaat ctctcgtctt
     7621 atccagatgt tttatacgcc gtctaatacc caatctctcg tcttatccag atgttttata
     7681 cgccgagcca actttgtata gaaaagttgg ctccgaattt ctcgagggcc agtgccaagc
     7741 tttctttttt actttaatat tatgtatact gaaaaatgca cgtgatgata agactttgga
     7801 aatttgtgta aaacccccat ttttttttgc tgctgctatt gccaaagnca acaagtcttc
     7861 aggagacatc aacactaagt ttctaccccg tcttccccta gaattctttc tgtacgctca
     7921 gggtgggcct ttaaaggata gcaccctacc gaagtcgact tctaagtaaa caccattact
     7981 aggagatgac taaatctgga aaacacatgg tggtctgaat gcgtctagtc tctgccataa
     8041 acataacatg tttgttttaa tgcattctcg tgtttaatcg acattaatgt gccgggagaa
     8101 agacatccca tccctgaaag gtttttccag ggaatagtca ggacgcattg gctttcactc
     8161 gaatttggcc ggcccacacc ggtagatttg gctgggcctc gaatttggcc ggcccacacc
     8221 ggtagatttg gctgggcctc gagaagaaga aaggaagaca tgtattacat tatccaacaa
     8281 aaaatggttc ttgacgtcta caaatcaaga atcttaaaga cattgaacga agtagctgaa
     8341 taaaaattat gaaaactata aaaactataa aaactgtact taagtcctca ataaaacata
     8401 aacttcttac tgtataaggt tttcgataat ttcttacttg attctaggag agcaacggta
     8461 ataaaagcaa cgacggggaa ttcgacccaa gtttgtacaa aaaagcaggc tggccaccat
     8521 gtctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga
     8581 tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa
     8641 attgacctta aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt
     8701 cactacttta acttatggtg ttcaatgttt ttctagatac ccagatcata tgaaacaaca
     8761 tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa
     8821 agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa
     8881 tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt
     8941 ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat
     9001 caaagttaac ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca
     9061 ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt
     9121 atccactcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt
     9181 agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca aataataata
     9241 cccagctttc ttgtacaaag tggtctacag agtactgaca ataaaaagat tcttgttttc
     9301 aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt
     9361 agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg
     9421 cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt
     9481 cgatactaac gccgccatcc agtttaaacg agctcgaatt catcgatgat atcagatcca
     9541 ctactgaggt gaattccctt atgtgagtgt aaaaggcagg cgagtttgtc ccgtgaattc
     9601 ccttatgtga gtgtaaaagg caggcgagtt tgtcccgagc caactttgta tagaaaagtt
     9661 ggctccgaat ttctcgagcg atactaacgc cgccatccag tgtcgaaaac gagctcatag
     9721 cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc
     9781 cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg
     9841 gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt
     9901 tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt
     9961 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt
    10021 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
    10081 tcattagaaa gaaagcatag caatctaatc taagttttct agaactagtg gatccccccc
    10141 ggacgacaga gaattcgacc caagtttgta caaaaaagca ggctgaaaaa aaatgggtaa
    10201 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
    10261 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
    10321 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
    10381 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
    10441 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
    10501 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
    10561 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
    10621 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
    10681 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
    10741 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
    10801 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
    10861 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
    10921 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
    10981 gatgctcgat gagtttttct aatacccagc tttcttgtac aaagtggtct acgaacacta
    11041 ctcattacaa cgccagtcta ttgagacaat agttttgtat aactaaataa tattggaaac
    11101 taaatacgaa tacccaaatt ttttatctaa attttgccga aagattaaaa tctgcagctg
    11161 aggttgcttg caaaagcagt aattggaaag cactctcaaa gaatcctctg gcccgtgtct
    11221 caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg
    11281 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
    11341 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
    11401 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
    11461 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
    11521 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
    11581 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
    11641 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
    11701 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
    11761 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
    11821 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
    11881 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
    11941 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
    12001 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
    12061 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
    12121 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
    12181 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
    12241 ga
//
