LOCUS       40924_27643        4105 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLDR9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Wiencis AM, Keagle P, Andersen J, Wotanis J, Newcombe R, August PR.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2000) Molecular Genomics, Aventis Pharmaceuticals,
            26 Landsdowne Street, Cambridge, MA 02139, USA
REFERENCE   2  (bases 1 to 4105)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2000) Molecular Genomics, Aventis Pharmaceuticals, 26 
            Landsdowne Street, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             216..1028
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        1423..1527
                     /label=AmpR promoter
     CDS             1528..2385
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    complement(2622..2853)
                     /label=lambda attP
                     /note="integrase from phage lambda"
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 accagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat
       61 gcgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt
      121 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
      181 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
      241 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      301 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
      361 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      421 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      481 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
      541 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      601 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
      661 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
      721 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct
      781 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
      841 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
      901 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
      961 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1021 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     1081 gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     1141 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     1201 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
     1261 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcacacagg
     1321 tgcggttgct gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1381 tttcttaggc ggccgccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1501 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1621 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1681 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1741 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     1801 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1861 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1921 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1981 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2041 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2101 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2161 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2221 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2281 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2341 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2401 gtttactcat atatacttta gattgattta attctcatgt ttgacagctt atcatcgata
     2461 agcttgggct gcaggtcgac tctagaggat ccccgggcga gctcgaattc gaagcagctt
     2521 tgcactggat tgcgaggctt tgtgcttctc tggagtgcga caggtttgat gacaaaaaat
     2581 tagcgcaaga agacaaaaat caccttgcgc taatgctctg ttacaggtca ctaataccat
     2641 ctaagtagtt gattcatagt gactgcatat gttgtgtttt acagtattat gtagtctgtt
     2701 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2761 cttttttata ctaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac
     2821 aggtcactat cagtcaaaat aaaatcatta tttgatttca attttgtccc actccctgcc
     2881 tctgtcatca cgatactgtg atgccatggt gtccgactta tgcccgagaa gatgttgagc
     2941 aaacttatcg cttatctgct tctcatagag tcttgcagac aaactgcgca actcgtgaaa
     3001 ggtaggcgga tctattctta ctcctagatc ttcctatgcg gtgtgaaata ccgcacagat
     3061 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     3121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3181 ccacagaatc aggggataac gcaggaaaga agcggccgct gagcaaaagg ccagcaaaag
     3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3961 acctagatcc ttttattctt gaagacgaaa gggtgaggtc tgcctcgtga agaaggtgtt
     4021 gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg
     4081 ttgatgagag ctttgttgta ggtgg
//
