LOCUS       62056_13760        8108 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLCT1, complete sequence.
ACCESSION   MK176920
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1291..1302
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     primer_bind     2669..2685
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2735..2831)
                     /regulatory_class="promoter"
     primer_bind     complement(2852..2868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2876..2892)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2900..2930)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2945..2966)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3254..3842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4095..4895
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(5332..5700)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5733..5842)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6080..6091)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    6162..6261
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             6271..8085
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagttt aaactccgcg aacggctcat caggtacggc gtgccgacgg
      121 tcgaggcggg cggcgtatgg gccaaccagg aagggccggt aagcgtcacg gcacgcgccg
      181 actgagagac gtttccgcag gtcaaccccg ttccagccca acagtgttag tctttgctct
      241 tacccagttg ggcgggatag cctgcccggc atgagcgtga aggttgaagg catggtcatt
      301 ctggcaggcg gctacgaccg acagtcggcg gaacgggaga acagttcgac cgcttcaccg
      361 gccacccagc gcgccgcgaa ccgggggaag gctgaggcgc tggcgaagga gtacgcgcgc
      421 gacggcgtcg aggtgaagtg gctgggtcac ttcagcgaag cgcccggcac gtcggcattc
      481 acgggcgtcg accggccgga gttcaaccgg attttggaca tgtgccggaa ccgggaaatg
      541 aacatgatca ttgttcatta catttcgcgc ctcagccgcg aagagccgct ggacattatt
      601 ccggtcgtca cggaattgct ccggctgggc gtgaccattg tcagcgtgaa cgaaggcaca
      661 ttccgccccg gcgaaatgat ggaccttatt cacctgatca tgcgccttca ggcttcgcat
      721 gatgagtcga agaacaagag cgtcgccgtg tcgaacgcta aggaattggc gaagcggctg
      781 ggcggacaca cggggtcgac gccgtacgga ttcgacacgg tcgaggaaat ggttccgaac
      841 ccggaagacg gcggaaagct ggttgccatt cgccgactgg tgcccagcgc gcacacctgg
      901 gaaggcgcac acggcagcga aggggcggta atccgctggg cgtggcagga gatcaagacg
      961 caccgcgata cgccattcaa gggtggcgga gccgggtcgt ttcaccctgg gtcgctgaac
     1021 gggctttgtg agcggctgta ccgcgacaag gtgcctacgc gcggcacgct ggtcggtaag
     1081 aagcgcgccg gttccgattg ggaccccggc gttttgaagc gcgtactcag cgacccgcgc
     1141 attgccgggt atcaagctga catcgcatac aaggtgcgcg ccgacggttc gcggggcggc
     1201 ttcagccatt acaagatcag gcgcgacccg gtcaccatgg agccgctgac cctgcccggc
     1261 ttcgagccgt acattccccc ggcggaatgg tgggaacttc aggagtggct tcagggtcga
     1321 ggacgcggga agggtcagta ccgggggcaa tcgctcctgt cggcaatgga cgtcctttac
     1381 tgctacggct ccggccagct cgacccggag acgggttaca gcaacgggtc gaccatggcg
     1441 ggcaacgtcc gcgaaggtga tcaagctcac aagtcgtcgt acgcgtgcaa gtgcccccgc
     1501 cgggttcatg acgggtcgtc atgctcgatc acgatgcaca accttgaccc gtacatcgtc
     1561 ggcgcgatct tcgcgcgcat cacggccttc gaccctgccg accctgacga cctcgaaggc
     1621 gacacggcag cgctcatgta cgaagccgca cggcgctggg gagcgacgca cgaacgcccg
     1681 gagttgaagg gtcagcgctc cgaactgatg gcacagcgcg cggacgccgt gaaggcgctc
     1741 gaagagcttt acgaagacaa gcggaacggc ggctaccggt ccgccatggg acggcgcgcg
     1801 tttctcgaag aggaagccgc gctgacgctc cgcatggaag gggccgaaga acggcttcgt
     1861 cagctcgacg ccgccgactc ccccgtgctg ccgatcggcg aatggctggg cgaccggggc
     1921 agcgacccga cgggaccggg ttcgtggtgg gcgctagcgc cccttgaaga ccgtcgggcg
     1981 ttcgtccggc tcttcgtgga ccggatcgag gtgatcaagc ttccgaaggg cgttcagcgg
     2041 cccggacggg ttcccccgat cgccgaccgt gtgcgtatcc actgggcgaa gccgaaggtc
     2101 gaggaagaga cggagccgga gacgctgaac gggttcacag cggcggcgtg acggcggcac
     2161 cagcgcaacg ggaaggggct tcggcccctt ttctcgtgcc cggcgtcggt tcgttgccct
     2221 aagcaactgt tcctagcgtc acgtcaatgc atatgcggcc gccgcaattg atttaaattg
     2281 aagccgccga agccccgaag cttccccttg accaatacta gtgaatctcg tgctttcagc
     2341 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     2401 aaagatcgtt atgttgatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     2461 gacattgggg aatttatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2521 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     2581 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     2641 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgggctg
     2701 caggtcgact ctagaggatc cttaattaag gcatatggac actccttact tagactgtcg
     2761 tattctcctg gccacgactt tacaacaccg cacagcatgt tgtcaaagca gagacggttc
     2821 gaatgtgaac agatatcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     2881 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2941 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3841 aagaagatcc tttgatctac tagtagctcc atcagcaaaa ggggatgata agtttatcac
     3901 caatttaaat ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tggttcatgt gcagctccat
     4021 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     4081 gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa
     4141 aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg
     4201 tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg
     4261 ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg
     4321 acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg
     4381 acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag
     4441 cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct cactcgcctg
     4501 caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac
     4561 acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc
     4621 cgagacactg caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg
     4681 accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga
     4741 aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga
     4801 cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat
     4861 gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat
     4921 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtggagcg gatcggggat
     4981 tgtctttctt cagctcgctg atgatatgct gacgctcaat gccgtttggc ctccgactaa
     5041 cgaaaatccc gcatttggac ggctgatccg attggcacgg cggacggcga atggcggagc
     5101 agacgctcgt ccgggggcaa tgagatatga aaaagcctga actcaccgcg acgtatcggg
     5161 ccctggccag ctagctagag tcgacctgca ggtccccggg gatcggtctt gccttgctcg
     5221 tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg catggggacg
     5281 tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc
     5341 cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt
     5401 cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag
     5461 ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg
     5521 gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
     5581 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
     5641 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat
     5701 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
     5761 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     5821 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
     5881 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
     5941 tataatgacc ccgaagcagg gttatgcagc ggaaaagatc cgtcgacctg caggcatgca
     6001 agctctagcg attccagacg tcccgaaggc gtggcgcggc ttccccgtgc cggagcaatc
     6061 gccctgggtg ggttacacga cgcccctcta tggcccgtac tgacggacac accgaagccc
     6121 cggcggcaac cctcagcgga tgccccgggg cttcacgttt tcccaggtca gaagcggttt
     6181 tcgggagtag tgccccaact ggggtaacct ttgagttctc tcagttgggg gcgtagggtc
     6241 gccgacatga cacaaggggt tgtgaccggg gtggacacgt acgcgggtgc ttacgaccgt
     6301 cagtcgcgcg agcgcgaaaa ttcgagcgca gcaagcccag cgacacagcg tagcgccaac
     6361 gaagacaagg cggccgacct tcagcgcgaa gtcgagcgcg acgggggccg gttcaggttc
     6421 gtcgggcatt tcagcgaagc gccgggcacg tcggcgttcg ggacggcgga gcgcccggag
     6481 ttcgaacgca tcctgaacga atgccgcgcc gggcggctca acatgatcat tgtctatgac
     6541 gtgtcgcgct tctcgcgcct gaaggtcatg gacgcgattc cgattgtctc ggaattgctc
     6601 gccctgggcg tgacgattgt ttccactcag gaaggcgtct tccggcaggg aaacgtcatg
     6661 gacctgattc acctgattat gcggctcgac gcgtcgcaca aagaatcttc gctgaagtcg
     6721 gcgaagattc tcgacacgaa gaaccttcag cgcgaattgg gcgggtacgt cggcgggaag
     6781 gcgccttacg gcttcgagct tgtttcggag acgaaggaga tcacgcgcaa cggccgaatg
     6841 gtcaatgtcg tcatcaacaa gcttgcgcac tcgaccactc cccttaccgg acccttcgag
     6901 ttcgagcccg acgtaatccg gtggtggtgg cgtgagatca agacgcacaa acaccttccc
     6961 ttcaagccgg gcagtcaagc cgccattcac ccgggcagca tcacggggct ttgtaagcgc
     7021 atggacgctg acgccgtgcc gacccggggc gagacgattg ggaagaagac cgcttcaagc
     7081 gcctgggacc cggcaaccgt tatgcgaatc cttcgggacc cgcgtattgc gggcttcgcc
     7141 gctgaggtga tctacaagaa gaagccggac ggcacgccga ccacgaagat tgagggttac
     7201 cgcattcagc gcgacccgat cacgctccgg ccggtcgagc ttgattgcgg accgatcatc
     7261 gagcccgctg agtggtatga gcttcaggcg tggttggacg gcagggggcg cggcaagggg
     7321 ctttcccggg ggcaagccat tctgtccgcc atggacaagc tgtactgcga gtgtggcgcc
     7381 gtcatgactt cgaagcgcgg ggaagaatcg atcaaggact cttaccgctg ccgtcgccgg
     7441 aaggtggtcg acccgtccgc acctgggcag cacgaaggca cgtgcaacgt cagcatggcg
     7501 gcactcgaca agttcgttgc ggaacgcatc ttcaacaaga tcaggcacgc cgaaggcgac
     7561 gaagagacgt tggcgcttct gtgggaagcc gcccgacgct tcggcaagct cactgaggcg
     7621 cctgagaaga gcggcgaacg ggcgaacctt gttgcggagc gcgccgacgc cctgaacgcc
     7681 cttgaagagc tgtacgaaga ccgcgcggca ggcgcgtacg acggacccgt tggcaggaag
     7741 cacttccgga agcaacaggc agcgctgacg ctccggcagc aaggggcgga agagcggctt
     7801 gccgaacttg aagccgccga agccccgaag cttccccttg accaatggtt ccccgaagac
     7861 gccgacgctg acccgaccgg ccctaagtcg tggtgggggc gcgcgtcagt agacgacaag
     7921 cgcgtgttcg tcgggctctt cgtagacaag atcgttgtca cgaagtcgac tacgggcagg
     7981 gggcagggaa cgcccatcga gaagcgcgct tcgatcacgt gggcgaagcc gccgaccgac
     8041 gacgacgaag acgacgccca ggacggcacg gaagacgtag cggcgtagcg agacacccgg
     8101 gaagcctg
//
