LOCUS       62056_13740        9879 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLCBR1, complete sequence.
ACCESSION   MK176924
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             220..2004
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69784.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     CDS             complement(3663..3674)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     primer_bind     4440..4456
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4623..4639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4647..4663)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4671..4701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4716..4737)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5025..5613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5866..6666
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(7103..7471)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(7504..7613)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(7851..7862)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    7933..8032
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             8042..9856
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagttt aaactggcgc cggacggggc ttcagacgtt tcgggtgctg
      121 ggttgttgtc tctggacagt gatccatggg aaactactca gcaccaccaa tgttcccaaa
      181 agaaagcgca ggtcagcgcc catgagccaa gatctaggca tgtcgccctt catcgctccc
      241 gacgtccctg agcaccttct agacactgtt cgcgtcttcc tgtacgcgcg tcagtctaag
      301 ggccggtccg acggctcaga cgtgtcgacc gaagcacagc tagcggccgg tcgtgcgttg
      361 gtcgcgtctc gcaacgccca ggggggtgcg cgctgggtcg tggcaggtga gttcgtggac
      421 gtcgggcgct ccggctggga cccgaacgtg acccgtgccg acttcgagcg catgatgggc
      481 gaagtccgcg ccggcgaagg tgacgttgtc gttgtgaatg agctttcccg gctcactcgc
      541 aagggcgccc atgacgcgct cgaaatcgac aacgaattga agaagcacgg cgtgcgcttc
      601 atgtcggttc ttgagccgtt ccttgacacg tctaccccta tcggcgtcgc cattttcgcg
      661 ctgatcgctg cccttgcgaa acaggacagt gacctgaagg cggagcgcct gaagggtgcg
      721 aaagacgaga ttgccgcgct gggtggcgtt cactcgtctt ccgccccgtt cggaatgcgc
      781 gccgtgcgca agaaggtcga taatctcgtg atctccgttc ttgagccgga cgaagacaac
      841 ccggatcacg tcgagctagt tgagcgcatg gcgaaaatgt cgttcgaagg cgtgtccgac
      901 aacgccattg caacgacctt cgagaaggaa aagatcccgt cgcccggaat ggctgagaga
      961 cgcgccacgg aaaagcgtct tgcgtccatc aaggcacgtc gcctgaacgg cgctgaaaag
     1021 ccgatcatgt ggcgcgctca aacggtccga tggattctca accatcccgc aatcggcggt
     1081 ttcgcattcg agcgtgtgaa gcacggtaag gcgcacatca acgtcatacg gcgcgacccc
     1141 ggcggcaagc cgctaacgcc ccacacgggc attctcagcg gctcgaagtg gcttgagctt
     1201 caagagaagc gttccgggaa gaatctcagc gaccggaagc ctggggccga agtcgaaccg
     1261 acgcttctga gcgggtggcg tttcctgggg tgccgaatct gcggcggctc aatgggtcag
     1321 tcccagggtg gccgtaagcg caacggcgac cttgccgaag gcaattacat gtgcgccaac
     1381 ccgaaggggc acggcggctt gtcggtcaag cgcagcgaac tggacgaatt cgttgcttcg
     1441 aaggtgtggg cacggctccg cacagccgac atggaagatg aacacgatca ggcatggatt
     1501 gccgccgctg cggagcgctt cgcccttcag cacgacctag cgggggtggc cgatgagcgg
     1561 cgcgaacaac aggcgcacct agacaacgtg cggcgctcca tcaaggacct tcaggcggac
     1621 cgtaaggccg gtctgtacgt cgggcgtgaa gagctggaaa cgtggcgctc aacggtgctg
     1681 caataccggt cctacgaagc ggagtgcacg acccgactcg ctgagcttga cgagaagatg
     1741 aacggcagca cccgcgttcc gtctgagtgg ttcagcggcg aagacccgac ggccgaaggg
     1801 ggcatctggg caagctggga cgtgtacgag cgtcgggagt tcctgagctt cttccttgac
     1861 tccgtcatgg tcgaccgggg gcgccaccct gagacgaaga aatacatccc cctgaaggac
     1921 cgtgtgacgc tcaagtgggc ggagctgctg aaggaggaag acgaagcgag cgaagccact
     1981 gagcgggagc ttgcggcgct gtagcgcaca gcgggagggg tcgagccggc ggacggttcg
     2041 gccccttttt tggccttgaa atcgttagtt aggctaatgc atttgcaccg tcctcgctgg
     2101 agtcgctgac gaggcgcgtg gtgggcacgt ggagggctgc ggccagttgc cgggcggtct
     2161 ccagacgggt gtcgctacgc tccccttgct ccagcttccg gatcagcgag agggacaccc
     2221 cggagctctt ggcgagctct tgctgactca tgccccgacg cttgcgtacg tcgcggagct
     2281 gcttaccaat gtcggcactc atgagtcaag actacggcgc gtaagctggt gagcacatgg
     2341 aaaagccccc gcttcctggc gggggcgagg gtccgaggca tgttccccaa agcgatacca
     2401 cttgaagcag tggtactgct tgtgggtaca ctctgcgggt gatgaatcga ggggggccca
     2461 ctgtacgggc cgacatctac gtccgaatca gcctggaccg cacaggggaa gagctcgggg
     2521 tcgagcgcca ggaggagtcg tgtcgcgagc tctgcaagag cctcggcatg gaggtggggc
     2581 aggtgtgggt cgacaacgac ctgagcgcca ccaagaagaa cgtcgtccgc cctgacttcg
     2641 aggcgatgat cgcgagcaac ccgcaggcga tcgtctgctg gcacaccgac cggctcatcc
     2701 gcgtcacgcg ggacctggag cgggtgatcg acctcggagt caacgtccac gccgtgatgg
     2761 ccggacacct ggacctgtcc accccggccg gccgagccgt cgcccgcacg gtgacggcct
     2821 gggccacgta cgagggcgag cagaaggctg agcgccagaa gctcgccaac atccagaacg
     2881 cccgcgccgg caagccgtac acccccggca tccgcccctt cgggtacggc gacgaccaca
     2941 tgaccatcgt gacggccgag gcggacgcca tccgcgacgg cgcgaagatg atcctcgacg
     3001 gctggtccct gtcggccgtg gctcgctact gggaggagct caagctccag tcgccccgga
     3061 gtatggccgc aggcggcaag ggctggtctc tgcggggcgt aaagaaggtg ctgacctccc
     3121 cgcgctacgt cgggcggtcc agctacctcg gggaggtcgt gggcgatgct cagtggccgc
     3181 ccatcctcga cccggacgtc tactacgggg tcgtggccat cctgaacaac cccgaccgct
     3241 tcagcggggg ccctcggacc ggccgcaccc ccggcacgct gctcgcaggc atcgccttgt
     3301 gcggtgagtg cggcaagacg gtcagtggac gcggctaccg aggtgtcctg gtctacggat
     3361 gtaaggacac gcacactcgg acgcctcgga gcatcgctga cggccgcgcg agcagctcga
     3421 ccctcgcccg gctcatgttc cccgacttcc tgcccggcct cctggcctct gggcaggccg
     3481 aggacggcca gtcggcagca tccaagcact cggaggccca gacgctgcgc gagcgccttg
     3541 acgggctggc tacggcctac gcggagggtg cgatcagcct gtctcagatg acggccggct
     3601 cggaagcact gcggaagaag ctggaggtga tcgaagccga cctcgtgggc tcggcaggca
     3661 tcccgccctt cgatccagtg gccggagtgg ctggcctgat ctccggctgg cccaccacgc
     3721 ctctcccgac gcgtcgagca tgggtggact tctgcctggt ggtcacgctg aacacccaga
     3781 aggggcgcca tgcgtcgagc atgaccgtgg acgaccacgt caccatcgag tggcgagacg
     3841 tggccgagta gcagatacga cgaagccccg gctaccccct tctgagggtg ccggggcttt
     3901 cgtgtgtcag ttgtggtggc cgctctccag gcggtcgatc tcttcgaggg acgcctcgta
     3961 gttgccggcc tcttggagga ggagcaggcg gaggtcggcg agcttgacct gcgcccacac
     4021 ggggagcggc cgccgcaatt gatttaaatt gaagccgccg aagccccgaa gcttcccctt
     4081 gaccaatact agtgaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     4141 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgttgatc ggcactttgc
     4201 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaatttatgc ggtgtgaaat
     4261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     4321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     4381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     4441 taaaacgacg gccagtgcca agcttgggct gcaggtcgac tctagaggat ccttaattaa
     4501 ggcatatgga cactccttac ttagactgtc gtattctcct ggccacgact ttacaacacc
     4561 gcacagcatg ttgtcaaagc agagacggtt cgaatgtgaa cagatatcga attcgtaatc
     4621 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatcta ctagtagctc
     5641 catcagcaaa aggggatgat aagtttatca ccaatttaaa tctacggggt ctgacgctca
     5701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5761 ctagatcctt ttggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc
     5821 accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg
     5881 gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac
     5941 cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc
     6001 ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctgcgctggg tccgggaggg
     6061 acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg
     6121 cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag
     6181 cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct
     6241 gatccattgc ccctgccacc tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc
     6301 gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc
     6361 gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc
     6421 aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg
     6481 gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct
     6541 cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg
     6601 ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg
     6661 attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt
     6721 gctggggcaa cacgtggagc ggatcgggga ttgtctttct tcagctcgct gatgatatgc
     6781 tgacgctcaa tgccgtttgg cctccgacta acgaaaatcc cgcatttgga cggctgatcc
     6841 gattggcacg gcggacggcg aatggcggag cagacgctcg tccgggggca atgagatatg
     6901 aaaaagcctg aactcaccgc gacgtatcgg gccctggcca gctagctaga gtcgacctgc
     6961 aggtccccgg ggatcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa
     7021 gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc
     7081 gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct
     7141 tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac
     7201 cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc
     7261 ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga
     7321 ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg
     7381 ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc
     7441 ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg
     7501 tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
     7561 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
     7621 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
     7681 cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
     7741 cggaaaagat ccgtcgacct gcaggcatgc aagctctagc gattccagac gtcccgaagg
     7801 cgtggcgcgg cttccccgtg ccggagcaat cgccctgggt gggttacacg acgcccctct
     7861 atggcccgta ctgacggaca caccgaagcc ccggcggcaa ccctcagcgg atgccccggg
     7921 gcttcacgtt ttcccaggtc agaagcggtt ttcgggagta gtgccccaac tggggtaacc
     7981 tttgagttct ctcagttggg ggcgtagggt cgccgacatg acacaagggg ttgtgaccgg
     8041 ggtggacacg tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgaaa attcgagcgc
     8101 agcaagccca gcgacacagc gtagcgccaa cgaagacaag gcggccgacc ttcagcgcga
     8161 agtcgagcgc gacgggggcc ggttcaggtt cgtcgggcat ttcagcgaag cgccgggcac
     8221 gtcggcgttc gggacggcgg agcgcccgga gttcgaacgc atcctgaacg aatgccgcgc
     8281 cgggcggctc aacatgatca ttgtctatga cgtgtcgcgc ttctcgcgcc tgaaggtcat
     8341 ggacgcgatt ccgattgtct cggaattgct cgccctgggc gtgacgattg tttccactca
     8401 ggaaggcgtc ttccggcagg gaaacgtcat ggacctgatt cacctgatta tgcggctcga
     8461 cgcgtcgcac aaagaatctt cgctgaagtc ggcgaagatt ctcgacacga agaaccttca
     8521 gcgcgaattg ggcgggtacg tcggcgggaa ggcgccttac ggcttcgagc ttgtttcgga
     8581 gacgaaggag atcacgcgca acggccgaat ggtcaatgtc gtcatcaaca agcttgcgca
     8641 ctcgaccact ccccttaccg gacccttcga gttcgagccc gacgtaatcc ggtggtggtg
     8701 gcgtgagatc aagacgcaca aacaccttcc cttcaagccg ggcagtcaag ccgccattca
     8761 cccgggcagc atcacggggc tttgtaagcg catggacgct gacgccgtgc cgacccgggg
     8821 cgagacgatt gggaagaaga ccgcttcaag cgcctgggac ccggcaaccg ttatgcgaat
     8881 ccttcgggac ccgcgtattg cgggcttcgc cgctgaggtg atctacaaga agaagccgga
     8941 cggcacgccg accacgaaga ttgagggtta ccgcattcag cgcgacccga tcacgctccg
     9001 gccggtcgag cttgattgcg gaccgatcat cgagcccgct gagtggtatg agcttcaggc
     9061 gtggttggac ggcagggggc gcggcaaggg gctttcccgg gggcaagcca ttctgtccgc
     9121 catggacaag ctgtactgcg agtgtggcgc cgtcatgact tcgaagcgcg gggaagaatc
     9181 gatcaaggac tcttaccgct gccgtcgccg gaaggtggtc gacccgtccg cacctgggca
     9241 gcacgaaggc acgtgcaacg tcagcatggc ggcactcgac aagttcgttg cggaacgcat
     9301 cttcaacaag atcaggcacg ccgaaggcga cgaagagacg ttggcgcttc tgtgggaagc
     9361 cgcccgacgc ttcggcaagc tcactgaggc gcctgagaag agcggcgaac gggcgaacct
     9421 tgttgcggag cgcgccgacg ccctgaacgc ccttgaagag ctgtacgaag accgcgcggc
     9481 aggcgcgtac gacggacccg ttggcaggaa gcacttccgg aagcaacagg cagcgctgac
     9541 gctccggcag caaggggcgg aagagcggct tgccgaactt gaagccgccg aagccccgaa
     9601 gcttcccctt gaccaatggt tccccgaaga cgccgacgct gacccgaccg gccctaagtc
     9661 gtggtggggg cgcgcgtcag tagacgacaa gcgcgtgttc gtcgggctct tcgtagacaa
     9721 gatcgttgtc acgaagtcga ctacgggcag ggggcaggga acgcccatcg agaagcgcgc
     9781 ttcgatcacg tgggcgaagc cgccgaccga cgacgacgaa gacgacgccc aggacggcac
     9841 ggaagacgta gcggcgtagc gagacacccg ggaagcctg
//
