LOCUS       62056_13730        5948 bp DNA     circular SYN 12-MAY-2019
DEFINITION  Cloning vector pLC1, complete sequence.
ACCESSION   MK159855
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..5948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     509..525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(575..671)
                     /regulatory_class="promoter"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(716..732)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(740..770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(785..806)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1094..1682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1935..2735
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(3172..3540)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(3573..3682)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3920..3931)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    4002..4101
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             4111..5925
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagttt aaacgcatgc atatgcggcc gccgcaattg atttaaattg
      121 aagccgccga agccccgaag cttccccttg accaatacta gtgaatctcg tgctttcagc
      181 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
      241 aaagatcgtt atgttgatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
      301 gacattgggg aatttatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      361 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      421 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      481 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgggctg
      541 caggtcgact ctagaggatc cttaattaag gcatatggac actccttact tagactgtcg
      601 tattctcctg gccacgactt tacaacaccg cacagcatgt tgtcaaagca gagacggttc
      661 gaatgtgaac agatatcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
      721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1681 aagaagatcc tttgatctac tagtagctcc atcagcaaaa ggggatgata agtttatcac
     1741 caatttaaat ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tggttcatgt gcagctccat
     1861 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     1921 gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa
     1981 aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg
     2041 tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg
     2101 ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg
     2161 acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg
     2221 acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag
     2281 cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct cactcgcctg
     2341 caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac
     2401 acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc
     2461 cgagacactg caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg
     2521 accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga
     2581 aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga
     2641 cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat
     2701 gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat
     2761 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtggagcg gatcggggat
     2821 tgtctttctt cagctcgctg atgatatgct gacgctcaat gccgtttggc ctccgactaa
     2881 cgaaaatccc gcatttggac ggctgatccg attggcacgg cggacggcga atggcggagc
     2941 agacgctcgt ccgggggcaa tgagatatga aaaagcctga actcaccgcg acgtatcggg
     3001 ccctggccag ctagctagag tcgacctgca ggtccccggg gatcggtctt gccttgctcg
     3061 tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg catggggacg
     3121 tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc
     3181 cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt
     3241 cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag
     3301 ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg
     3361 gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
     3421 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
     3481 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat
     3541 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
     3601 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     3661 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
     3721 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
     3781 tataatgacc ccgaagcagg gttatgcagc ggaaaagatc cgtcgacctg caggcatgca
     3841 agctctagcg attccagacg tcccgaaggc gtggcgcggc ttccccgtgc cggagcaatc
     3901 gccctgggtg ggttacacga cgcccctcta tggcccgtac tgacggacac accgaagccc
     3961 cggcggcaac cctcagcgga tgccccgggg cttcacgttt tcccaggtca gaagcggttt
     4021 tcgggagtag tgccccaact ggggtaacct ttgagttctc tcagttgggg gcgtagggtc
     4081 gccgacatga cacaaggggt tgtgaccggg gtggacacgt acgcgggtgc ttacgaccgt
     4141 cagtcgcgcg agcgcgaaaa ttcgagcgca gcaagcccag cgacacagcg tagcgccaac
     4201 gaagacaagg cggccgacct tcagcgcgaa gtcgagcgcg acgggggccg gttcaggttc
     4261 gtcgggcatt tcagcgaagc gccgggcacg tcggcgttcg ggacggcgga gcgcccggag
     4321 ttcgaacgca tcctgaacga atgccgcgcc gggcggctca acatgatcat tgtctatgac
     4381 gtgtcgcgct tctcgcgcct gaaggtcatg gacgcgattc cgattgtctc ggaattgctc
     4441 gccctgggcg tgacgattgt ttccactcag gaaggcgtct tccggcaggg aaacgtcatg
     4501 gacctgattc acctgattat gcggctcgac gcgtcgcaca aagaatcttc gctgaagtcg
     4561 gcgaagattc tcgacacgaa gaaccttcag cgcgaattgg gcgggtacgt cggcgggaag
     4621 gcgccttacg gcttcgagct tgtttcggag acgaaggaga tcacgcgcaa cggccgaatg
     4681 gtcaatgtcg tcatcaacaa gcttgcgcac tcgaccactc cccttaccgg acccttcgag
     4741 ttcgagcccg acgtaatccg gtggtggtgg cgtgagatca agacgcacaa acaccttccc
     4801 ttcaagccgg gcagtcaagc cgccattcac ccgggcagca tcacggggct ttgtaagcgc
     4861 atggacgctg acgccgtgcc gacccggggc gagacgattg ggaagaagac cgcttcaagc
     4921 gcctgggacc cggcaaccgt tatgcgaatc cttcgggacc cgcgtattgc gggcttcgcc
     4981 gctgaggtga tctacaagaa gaagccggac ggcacgccga ccacgaagat tgagggttac
     5041 cgcattcagc gcgacccgat cacgctccgg ccggtcgagc ttgattgcgg accgatcatc
     5101 gagcccgctg agtggtatga gcttcaggcg tggttggacg gcagggggcg cggcaagggg
     5161 ctttcccggg ggcaagccat tctgtccgcc atggacaagc tgtactgcga gtgtggcgcc
     5221 gtcatgactt cgaagcgcgg ggaagaatcg atcaaggact cttaccgctg ccgtcgccgg
     5281 aaggtggtcg acccgtccgc acctgggcag cacgaaggca cgtgcaacgt cagcatggcg
     5341 gcactcgaca agttcgttgc ggaacgcatc ttcaacaaga tcaggcacgc cgaaggcgac
     5401 gaagagacgt tggcgcttct gtgggaagcc gcccgacgct tcggcaagct cactgaggcg
     5461 cctgagaaga gcggcgaacg ggcgaacctt gttgcggagc gcgccgacgc cctgaacgcc
     5521 cttgaagagc tgtacgaaga ccgcgcggca ggcgcgtacg acggacccgt tggcaggaag
     5581 cacttccgga agcaacaggc agcgctgacg ctccggcagc aaggggcgga agagcggctt
     5641 gccgaacttg aagccgccga agccccgaag cttccccttg accaatggtt ccccgaagac
     5701 gccgacgctg acccgaccgg ccctaagtcg tggtgggggc gcgcgtcagt agacgacaag
     5761 cgcgtgttcg tcgggctctt cgtagacaag atcgttgtca cgaagtcgac tacgggcagg
     5821 gggcagggaa cgcccatcga gaagcgcgct tcgatcacgt gggcgaagcc gccgaccgac
     5881 gacgacgaag acgacgccca ggacggcacg gaagacgtag cggcgtagcg agacacccgg
     5941 gaagcctg
//
