LOCUS       62056_13725        7915 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLBT1, complete sequence.
ACCESSION   MK176918
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..7915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1215..1226
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     primer_bind     2593..2609
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2659..2755)
                     /regulatory_class="promoter"
     primer_bind     complement(2776..2792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2800..2816)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2824..2854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2869..2890)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3178..3766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4019..4819
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(5256..5624)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5657..5766)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             6059..7843
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69765.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
ORIGIN
        1 gtttaaactc cgcgaacggc tcatcaggta cggcgtgccg acggtcgagg cgggcggcgt
       61 atgggccaac caggaagggc cggtaagcgt cacggcacgc gccgactgag agacgtttcc
      121 gcaggtcaac cccgttccag cccaacagtg ttagtctttg ctcttaccca gttgggcggg
      181 atagcctgcc cggcatgagc gtgaaggttg aaggcatggt cattctggca ggcggctacg
      241 accgacagtc ggcggaacgg gagaacagtt cgaccgcttc accggccacc cagcgcgccg
      301 cgaaccgggg gaaggctgag gcgctggcga aggagtacgc gcgcgacggc gtcgaggtga
      361 agtggctggg tcacttcagc gaagcgcccg gcacgtcggc attcacgggc gtcgaccggc
      421 cggagttcaa ccggattttg gacatgtgcc ggaaccggga aatgaacatg atcattgttc
      481 attacatttc gcgcctcagc cgcgaagagc cgctggacat tattccggtc gtcacggaat
      541 tgctccggct gggcgtgacc attgtcagcg tgaacgaagg cacattccgc cccggcgaaa
      601 tgatggacct tattcacctg atcatgcgcc ttcaggcttc gcatgatgag tcgaagaaca
      661 agagcgtcgc cgtgtcgaac gctaaggaat tggcgaagcg gctgggcgga cacacggggt
      721 cgacgccgta cggattcgac acggtcgagg aaatggttcc gaacccggaa gacggcggaa
      781 agctggttgc cattcgccga ctggtgccca gcgcgcacac ctgggaaggc gcacacggca
      841 gcgaaggggc ggtaatccgc tgggcgtggc aggagatcaa gacgcaccgc gatacgccat
      901 tcaagggtgg cggagccggg tcgtttcacc ctgggtcgct gaacgggctt tgtgagcggc
      961 tgtaccgcga caaggtgcct acgcgcggca cgctggtcgg taagaagcgc gccggttccg
     1021 attgggaccc cggcgttttg aagcgcgtac tcagcgaccc gcgcattgcc gggtatcaag
     1081 ctgacatcgc atacaaggtg cgcgccgacg gttcgcgggg cggcttcagc cattacaaga
     1141 tcaggcgcga cccggtcacc atggagccgc tgaccctgcc cggcttcgag ccgtacattc
     1201 ccccggcgga atggtgggaa cttcaggagt ggcttcaggg tcgaggacgc gggaagggtc
     1261 agtaccgggg gcaatcgctc ctgtcggcaa tggacgtcct ttactgctac ggctccggcc
     1321 agctcgaccc ggagacgggt tacagcaacg ggtcgaccat ggcgggcaac gtccgcgaag
     1381 gtgatcaagc tcacaagtcg tcgtacgcgt gcaagtgccc ccgccgggtt catgacgggt
     1441 cgtcatgctc gatcacgatg cacaaccttg acccgtacat cgtcggcgcg atcttcgcgc
     1501 gcatcacggc cttcgaccct gccgaccctg acgacctcga aggcgacacg gcagcgctca
     1561 tgtacgaagc cgcacggcgc tggggagcga cgcacgaacg cccggagttg aagggtcagc
     1621 gctccgaact gatggcacag cgcgcggacg ccgtgaaggc gctcgaagag ctttacgaag
     1681 acaagcggaa cggcggctac cggtccgcca tgggacggcg cgcgtttctc gaagaggaag
     1741 ccgcgctgac gctccgcatg gaaggggccg aagaacggct tcgtcagctc gacgccgccg
     1801 actcccccgt gctgccgatc ggcgaatggc tgggcgaccg gggcagcgac ccgacgggac
     1861 cgggttcgtg gtgggcgcta gcgccccttg aagaccgtcg ggcgttcgtc cggctcttcg
     1921 tggaccggat cgaggtgatc aagcttccga agggcgttca gcggcccgga cgggttcccc
     1981 cgatcgccga ccgtgtgcgt atccactggg cgaagccgaa ggtcgaggaa gagacggagc
     2041 cggagacgct gaacgggttc acagcggcgg cgtgacggcg gcaccagcgc aacgggaagg
     2101 ggcttcggcc ccttttctcg tgcccggcgt cggttcgttg ccctaagcaa ctgttcctag
     2161 cgtcacgtca atgcatatgc ggccgccgca attgatttaa attgaagccg ccgaagcccc
     2221 gaagcttccc cttgaccaat actagtgaat ctcgtgcttt cagcttcgat gtaggagggc
     2281 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg
     2341 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta
     2401 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2461 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2521 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2581 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag
     2641 gatccttaat taaggcatat ggacactcct tacttagact gtcgtattct cctggccacg
     2701 actttacaac accgcacagc atgttgtcaa agcagagacg gttcgaatgt gaacagatat
     2761 cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2821 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2881 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2941 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3001 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3061 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3121 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3181 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3241 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3301 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3361 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3421 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3481 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3541 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3601 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3661 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3721 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3781 ctactagtag ctccatcagc aaaaggggat gataagttta tcaccaattt aaatctacgg
     3841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3901 aaaggatctt cacctagatc cttttggttc atgtgcagct ccatcagcaa aaggggatga
     3961 taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg atcgactgat
     4021 gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg
     4081 tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca gctccttccg
     4141 tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc gtgctgcgct
     4201 gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg agccgttcga
     4261 tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat tctggcgcct
     4321 gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc cacaggcaga
     4381 gcagatcatc tctgatccat tgcccctgcc acctcactcg cctgcaagcc cggtcgcccg
     4441 tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg ccaacacgac
     4501 gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac actgcaccat
     4561 tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact gctgtgagcg
     4621 ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag gtccagtcgg
     4681 tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc tgggtcaact
     4741 gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc
     4801 cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt ggaggggcaa
     4861 ggtcgcgctg attgctgggg caacacgtgg agcggatcgg ggattgtctt tcttcagctc
     4921 gctgatgata tgctgacgct caatgccgtt tggcctccga ctaacgaaaa tcccgcattt
     4981 ggacggctga tccgattggc acggcggacg gcgaatggcg gagcagacgc tcgtccgggg
     5041 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtat cgggccctgg ccagctagct
     5101 agagtcgacc tgcaggtccc cggggatcgg tcttgccttg ctcgtcggtg atgtacttca
     5161 ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc
     5221 gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg gcggaccacg
     5281 cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg
     5341 atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg
     5401 ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg ctcatagtcc
     5461 acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg
     5521 ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg
     5581 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct
     5641 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     5701 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     5761 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     5821 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     5881 cagggttatg cagcggaaaa gatccgtcga cctgcaggca tgctggcgcc ggacggggct
     5941 tcagacgttt cgggtgctgg gttgttgtct ctggacagtg atccatggga aactactcag
     6001 caccaccaat gttcccaaaa gaaagcgcag gtcagcgccc atgagccaag atctaggcat
     6061 gtcgcccttc atcgctcccg acgtccctga gcaccttcta gacactgttc gcgtcttcct
     6121 gtacgcgcgt cagtctaagg gccggtccga cggctcagac gtgtcgaccg aagcacagct
     6181 agcggccggt cgtgcgttgg tcgcgtctcg caacgcccag gggggtgcgc gctgggtcgt
     6241 ggcaggtgag ttcgtggacg tcgggcgctc cggctgggac ccgaacgtga cccgtgccga
     6301 cttcgagcgc atgatgggcg aagtccgcgc cggcgaaggt gacgttgtcg ttgtgaatga
     6361 gctttcccgg ctcactcgca agggcgccca tgacgcgctc gaaatcgaca acgaattgaa
     6421 gaagcacggc gtgcgcttca tgtcggttct tgagccgttc cttgacacgt ctacccctat
     6481 cggcgtcgcc attttcgcgc tgatcgctgc ccttgcgaaa caggacagtg acctgaaggc
     6541 ggagcgcctg aagggtgcga aagacgagat tgccgcgctg ggtggcgttc actcgtcttc
     6601 cgccccgttc ggaatgcgcg ccgtgcgcaa gaaggtcgat aatctcgtga tctccgttct
     6661 tgagccggac gaagacaacc cggatcacgt cgagctagtt gagcgcatgg cgaaaatgtc
     6721 gttcgaaggc gtgtccgaca acgccattgc aacgaccttc gagaaggaaa agatcccgtc
     6781 gcccggaatg gctgagagac gcgccacgga aaagcgtctt gcgtccatca aggcacgtcg
     6841 cctgaacggc gctgaaaagc cgatcatgtg gcgcgctcaa acggtccgat ggattctcaa
     6901 ccatcccgca atcggcggtt tcgcattcga gcgtgtgaag cacggtaagg cgcacatcaa
     6961 cgtcatacgg cgcgaccccg gcggcaagcc gctaacgccc cacacgggca ttctcagcgg
     7021 ctcgaagtgg cttgagcttc aagagaagcg ttccgggaag aatctcagcg accggaagcc
     7081 tggggccgaa gtcgaaccga cgcttctgag cgggtggcgt ttcctggggt gccgaatctg
     7141 cggcggctca atgggtcagt cccagggtgg ccgtaagcgc aacggcgacc ttgccgaagg
     7201 caattacatg tgcgccaacc cgaaggggca cggcggcttg tcggtcaagc gcagcgaact
     7261 ggacgaattc gttgcttcga aggtgtgggc acggctccgc acagccgaca tggaagatga
     7321 acacgatcag gcatggattg ccgccgctgc ggagcgcttc gcccttcagc acgacctagc
     7381 gggggtggcc gatgagcggc gcgaacaaca ggcgcaccta gacaacgtgc ggcgctccat
     7441 caaggacctt caggcggacc gtaaggccgg tctgtacgtc gggcgtgaag agctggaaac
     7501 gtggcgctca acggtgctgc aataccggtc ctacgaagcg gagtgcacga cccgactcgc
     7561 tgagcttgac gagaagatga acggcagcac ccgcgttccg tctgagtggt tcagcggcga
     7621 agacccgacg gccgaagggg gcatctgggc aagctgggac gtgtacgagc gtcgggagtt
     7681 cctgagcttc ttccttgact ccgtcatggt cgaccggggg cgccaccctg agacgaagaa
     7741 atacatcccc ctgaaggacc gtgtgacgct caagtgggcg gagctgctga aggaggaaga
     7801 cgaagcgagc gaagccactg agcgggagct tgcggcgctg tagcgcacag cgggaggggt
     7861 cgagccggcg gacggttcgg cccctttttt ggccttgaaa tcgttagtta ggcta
//
