LOCUS       62056_13720        7443 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLBS2, complete sequence.
ACCESSION   MK176922
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..7443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(137..153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(161..177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(185..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(230..251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(539..1127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1635..2426)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(3013..3122)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             3415..5199
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69777.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     CDS             complement(5503..7146)
                     /codon_start=1
                     /transl_table=11
                     /product="SV1 integrase"
                     /label=SV1 integrase
                     /protein_id="QCO69778.1"
                     /translation="MKRDLPSTFRGSRTPGEPWLGYIRVSTWREEKISPELQQSAIESW
                     AARTGRRIVDWIVDLDATGRNFKRKIMGGIQRVEGREAVGIAVWKFSRFGRNDLGIAIN
                     LARLEQAGGDLASATEEVDARTAVGRFNRAILFDLAVFESDRAGEQWKETHAHRRALKL
                     PATGRQRFGYVWHPRRVPDLTAPGGFRLQEERYERHPEFAPVAAELYERKLAGQGFSQL
                     AYWLNDELLIPTTRGNRWGTNTVQRYLDSGFAAGLLRVHDPECRCKLGQDHFSACKENR
                     WLWLPGAQPALIVPEQWKEYGAHREQTRKTPPRARRASYPTSGIMRHGHCRGTAVARSG
                     RDGKGGFVPGHVFVCFNRRNKGKSACEPGLYVRRDEVEAEVLKWLADTVADDIDNAPAL
                     PAQRTAPGTAPDPRARLVEERTRTEAELAKIEGALDRLVTDYALDPDKYPADTFGRVRD
                     QLLGKKGDIIKHLKSLSEVEVAPTREEFRPLIVGLLQEWDILHTTEKNAILRRLLRRLV
                     IHNRKSDQGAQWSVVRSFEFHPVWEPDPWS"
ORIGIN
        1 ggatccttaa ttaaggcata tggacactcc ttacttagac tgtcgtattc tcctggccac
       61 gactttacaa caccgcacag catgttgtca aagcagagac ggttcgaatg tgaacagata
      121 tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1201 tgagattatc aaaaaggatc ttcacctaga tccttttggt tcatgtgcag ctccatcagc
     1261 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     1321 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     1381 cgagctcggt acccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     1441 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     1501 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttact agtagctcca tcagcaaaag
     1561 gggatgataa gtttatcacc aatttaaatt gggcgtcgct tggtcggtca tttcgaaccc
     1621 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     1681 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     1741 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     1801 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     1861 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     1921 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     1981 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2041 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2101 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2161 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2221 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     2281 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     2341 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     2401 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     2461 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     2521 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     2581 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     2641 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     2701 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     2761 tgcttgcggc agcgtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     2821 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     2881 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     2941 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     3001 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     3061 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     3121 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     3181 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     3241 gttatgcagc ggaaaagatc cgtcgacctg caggcatgct ggcgccggac ggggcttcag
     3301 acgtttcggg tgctgggttg ttgtctctgg acagtgatcc atgggaaact actcagcacc
     3361 accaatgttc ccaaaagaaa gcgcaggtca gcgcccatga gccaagatct aggcatgtcg
     3421 cccttcatcg ctcccgacgt ccctgagcac cttctagaca ctgttcgcgt cttcctgtac
     3481 gcgcgtcagt ctaagggccg gtccgacggc tcagacgtgt cgaccgaagc acagctagcg
     3541 gccggtcgtg cgttggtcgc gtctcgcaac gcccaggggg gtgcgcgctg ggtcgtggca
     3601 ggtgagttcg tggacgtcgg gcgctccggc tgggacccga acgtgacccg tgccgacttc
     3661 gagcgcatga tgggcgaagt ccgcgccggc gaaggtgacg ttgtcgttgt gaatgagctt
     3721 tcccggctca ctcgcaaggg cgcccatgac gcgctcgaaa tcgacaacga attgaagaag
     3781 cacggcgtgc gcttcatgtc ggttcttgag ccgttccttg acacgtctac ccctatcggc
     3841 gtcgccattt tcgcgctgat cgctgccctt gcgaaacagg acagtgacct gaaggcggag
     3901 cgcctgaagg gtgcgaaaga cgagattgcc gcgctgggtg gcgttcactc gtcttccgcc
     3961 ccgttcggaa tgcgcgccgt gcgcaagaag gtcgataatc tcgtgatctc cgttcttgag
     4021 ccggacgaag acaacccgga tcacgtcgag ctagttgagc gcatggcgaa aatgtcgttc
     4081 gaaggcgtgt ccgacaacgc cattgcaacg accttcgaga aggaaaagat cccgtcgccc
     4141 ggaatggctg agagacgcgc cacggaaaag cgtcttgcgt ccatcaaggc acgtcgcctg
     4201 aacggcgctg aaaagccgat catgtggcgc gctcaaacgg tccgatggat tctcaaccat
     4261 cccgcaatcg gcggtttcgc attcgagcgt gtgaagcacg gtaaggcgca catcaacgtc
     4321 atacggcgcg accccggcgg caagccgcta acgccccaca cgggcattct cagcggctcg
     4381 aagtggcttg agcttcaaga gaagcgttcc gggaagaatc tcagcgaccg gaagcctggg
     4441 gccgaagtcg aaccgacgct tctgagcggg tggcgtttcc tggggtgccg aatctgcggc
     4501 ggctcaatgg gtcagtccca gggtggccgt aagcgcaacg gcgaccttgc cgaaggcaat
     4561 tacatgtgcg ccaacccgaa ggggcacggc ggcttgtcgg tcaagcgcag cgaactggac
     4621 gaattcgttg cttcgaaggt gtgggcacgg ctccgcacag ccgacatgga agatgaacac
     4681 gatcaggcat ggattgccgc cgctgcggag cgcttcgccc ttcagcacga cctagcgggg
     4741 gtggccgatg agcggcgcga acaacaggcg cacctagaca acgtgcggcg ctccatcaag
     4801 gaccttcagg cggaccgtaa ggccggtctg tacgtcgggc gtgaagagct ggaaacgtgg
     4861 cgctcaacgg tgctgcaata ccggtcctac gaagcggagt gcacgacccg actcgctgag
     4921 cttgacgaga agatgaacgg cagcacccgc gttccgtctg agtggttcag cggcgaagac
     4981 ccgacggccg aagggggcat ctgggcaagc tgggacgtgt acgagcgtcg ggagttcctg
     5041 agcttcttcc ttgactccgt catggtcgac cgggggcgcc accctgagac gaagaaatac
     5101 atccccctga aggaccgtgt gacgctcaag tgggcggagc tgctgaagga ggaagacgaa
     5161 gcgagcgaag ccactgagcg ggagcttgcg gcgctgtagc gcacagcggg aggggtcgag
     5221 ccggcggacg gttcggcccc ttttttggcc ttgaaatcgt tagttaggct agtttaaaca
     5281 gacgcggtgg gccgcacatg gaacggcccc ggtacctgag gtaggtaccg gggccgttct
     5341 tcgcgctccg atgtggtcct ttagatccac tgacgtgggt cagtgtctct aaaggactcg
     5401 cgagcatcgt tccccctctc cctgcactga ggtgcggttt tcagagggtg gcagcaggcg
     5461 gagaaacaat caagcgcgcc tgcatggttt gcgctccagc ggtcaagacc aggggtcggg
     5521 ctcccacacg ggatgaaact cgaagctgcg cacgacactc cactgtgcgc cctgatcgct
     5581 cttacggttg tggatgacca gacggcgcag caggcggcgc aggatggcgt tcttctcggt
     5641 ggtgtgcagg atgtcccact cctgcaagag cccgacgatc agggggcgga actcctcccg
     5701 cgtgggcgcg acttcaacct cgctgagcga cttcaagtgc ttgatgatgt cgcccttctt
     5761 accgagtagc tggtcgcgta cgcgcccgaa cgtgtccgcc gggtacttgt ccgggtccag
     5821 cgcgtaatcc gtgacgagac ggtcgagtgc accctcgatc ttggccaact cggcttccgt
     5881 gcgggtgcgc tcctcgacca ggcgggcccg cgggtccggc gcggtgcccg gcgcggtgcg
     5941 ctgagcggga agggccggcg cgttgtcgat gtcgtcggcg accgtgtcgg cgagccactt
     6001 caacacctcg gcctcgactt cgtcgcggcg cacgtagagg ccgggctcac aggccgactt
     6061 ccccttgttc ctgcggttga agcacacgaa cacgtggccc ggaacgaatc cgcccttccc
     6121 gtcgcggccg gatcgcgcaa cggccgtccc gcggcagtgt ccgtgccgca tgatgccgct
     6181 ggtcgggtat gaggcccggc gggcgcgcgg gggcgtcttg cgcgtctgct ctctgtgcgc
     6241 cccgtactcc ttccactgct cgggaacgat gagcgccggc tgtgccccgg ggagccagag
     6301 ccaccggttt tctttgcacg cagagaagtg gtcctgtccc agcttgcacc ggcactccgg
     6361 gtcgtggacg cgtagcaggc cggcggcgaa tccggagtcg aggtagcgct gaacggtgtt
     6421 ggtgccccag cggttgcccc gtgtggtggg gatgagtagt tcgtcgttca gccagtaggc
     6481 gagctgggag aacccctgtc cggcgagctt tcgctcgtag agttcggccg ccacgggggc
     6541 gaactctggg tgccgttcgt agcgctcctc ttgcaggcgg aacccgcccg gcgcggtgag
     6601 gtcgggtacg cgcctcgggt gccacacgta gccgaaccgc tgacgcccgg tggcggggag
     6661 tttcagggcc cgacggtggg cgtgcgtctc cttccactgt tcgccggccc gatccgactc
     6721 gaatacggcg aggtcgaaca gaatcgcgcg gttgaagcgt ccgacggccg tgcgggcgtc
     6781 gacttcttcc gtggcggacg cgaggtctcc gccggcctgt tcgaggcggg cgaggttgat
     6841 agcgatgccc aggtcgttcc ggccgaagcg gctgaacttc catacggcga ttccgacggc
     6901 ctcgcggccc tcgacgcgct ggatgccgcc catgatcttc cgcttgaagt tgcggcccgt
     6961 agcgtcgagg tcaacgatcc agtcgacgat ccgacgtccc gttcgggcgg cccatgactc
     7021 gatcgcggat tgctgtagct ccgggctgat cttctcctcg cgccatgtgc tgaccctgat
     7081 gtagccgagc cacggctcgc ccggcgtgcg ggagccgcgg aacgtgcttg gtaggtctcg
     7141 tttcatatgt ggatcctacc aaccggcacg attgtgccca caacagcatc gcggtgccac
     7201 gtgtggaccg cgtcggtcag atcctccccg cacctctcgc cagccgtcaa gatcgaccgc
     7261 gtgcacctgc gatcgccgat caaccgcgac tagcatcggg cgcaagccgc cactcgaacg
     7321 gacactcgca tgagatatgc atatgcggcc gccgcaattg atttaaattg aagccgccga
     7381 agccccgaag cttccccttg accaatacta gtagttcctt cgtcaccata gcgggcaggg
     7441 agc
//
