LOCUS       62056_13715        7329 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLBR2, complete sequence.
ACCESSION   MK176921
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(137..153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(161..177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(185..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(230..251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(539..1127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1635..2426)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(3013..3122)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             3415..5199
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69774.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     CDS             complement(6860..6871)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
ORIGIN
        1 ggatccttaa ttaaggcata tggacactcc ttacttagac tgtcgtattc tcctggccac
       61 gactttacaa caccgcacag catgttgtca aagcagagac ggttcgaatg tgaacagata
      121 tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1201 tgagattatc aaaaaggatc ttcacctaga tccttttggt tcatgtgcag ctccatcagc
     1261 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     1321 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     1381 cgagctcggt acccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     1441 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     1501 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttact agtagctcca tcagcaaaag
     1561 gggatgataa gtttatcacc aatttaaatt gggcgtcgct tggtcggtca tttcgaaccc
     1621 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     1681 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     1741 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     1801 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     1861 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     1921 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     1981 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2041 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2101 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2161 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2221 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     2281 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     2341 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     2401 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     2461 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     2521 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     2581 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     2641 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     2701 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     2761 tgcttgcggc agcgtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     2821 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     2881 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     2941 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     3001 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     3061 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     3121 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     3181 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     3241 gttatgcagc ggaaaagatc cgtcgacctg caggcatgct ggcgccggac ggggcttcag
     3301 acgtttcggg tgctgggttg ttgtctctgg acagtgatcc atgggaaact actcagcacc
     3361 accaatgttc ccaaaagaaa gcgcaggtca gcgcccatga gccaagatct aggcatgtcg
     3421 cccttcatcg ctcccgacgt ccctgagcac cttctagaca ctgttcgcgt cttcctgtac
     3481 gcgcgtcagt ctaagggccg gtccgacggc tcagacgtgt cgaccgaagc acagctagcg
     3541 gccggtcgtg cgttggtcgc gtctcgcaac gcccaggggg gtgcgcgctg ggtcgtggca
     3601 ggtgagttcg tggacgtcgg gcgctccggc tgggacccga acgtgacccg tgccgacttc
     3661 gagcgcatga tgggcgaagt ccgcgccggc gaaggtgacg ttgtcgttgt gaatgagctt
     3721 tcccggctca ctcgcaaggg cgcccatgac gcgctcgaaa tcgacaacga attgaagaag
     3781 cacggcgtgc gcttcatgtc ggttcttgag ccgttccttg acacgtctac ccctatcggc
     3841 gtcgccattt tcgcgctgat cgctgccctt gcgaaacagg acagtgacct gaaggcggag
     3901 cgcctgaagg gtgcgaaaga cgagattgcc gcgctgggtg gcgttcactc gtcttccgcc
     3961 ccgttcggaa tgcgcgccgt gcgcaagaag gtcgataatc tcgtgatctc cgttcttgag
     4021 ccggacgaag acaacccgga tcacgtcgag ctagttgagc gcatggcgaa aatgtcgttc
     4081 gaaggcgtgt ccgacaacgc cattgcaacg accttcgaga aggaaaagat cccgtcgccc
     4141 ggaatggctg agagacgcgc cacggaaaag cgtcttgcgt ccatcaaggc acgtcgcctg
     4201 aacggcgctg aaaagccgat catgtggcgc gctcaaacgg tccgatggat tctcaaccat
     4261 cccgcaatcg gcggtttcgc attcgagcgt gtgaagcacg gtaaggcgca catcaacgtc
     4321 atacggcgcg accccggcgg caagccgcta acgccccaca cgggcattct cagcggctcg
     4381 aagtggcttg agcttcaaga gaagcgttcc gggaagaatc tcagcgaccg gaagcctggg
     4441 gccgaagtcg aaccgacgct tctgagcggg tggcgtttcc tggggtgccg aatctgcggc
     4501 ggctcaatgg gtcagtccca gggtggccgt aagcgcaacg gcgaccttgc cgaaggcaat
     4561 tacatgtgcg ccaacccgaa ggggcacggc ggcttgtcgg tcaagcgcag cgaactggac
     4621 gaattcgttg cttcgaaggt gtgggcacgg ctccgcacag ccgacatgga agatgaacac
     4681 gatcaggcat ggattgccgc cgctgcggag cgcttcgccc ttcagcacga cctagcgggg
     4741 gtggccgatg agcggcgcga acaacaggcg cacctagaca acgtgcggcg ctccatcaag
     4801 gaccttcagg cggaccgtaa ggccggtctg tacgtcgggc gtgaagagct ggaaacgtgg
     4861 cgctcaacgg tgctgcaata ccggtcctac gaagcggagt gcacgacccg actcgctgag
     4921 cttgacgaga agatgaacgg cagcacccgc gttccgtctg agtggttcag cggcgaagac
     4981 ccgacggccg aagggggcat ctgggcaagc tgggacgtgt acgagcgtcg ggagttcctg
     5041 agcttcttcc ttgactccgt catggtcgac cgggggcgcc accctgagac gaagaaatac
     5101 atccccctga aggaccgtgt gacgctcaag tgggcggagc tgctgaagga ggaagacgaa
     5161 gcgagcgaag ccactgagcg ggagcttgcg gcgctgtagc gcacagcggg aggggtcgag
     5221 ccggcggacg gttcggcccc ttttttggcc ttgaaatcgt tagttaggct agtttaaact
     5281 tgcaccgtcc tcgctggagt cgctgacgag gcgcgtggtg ggcacgtgga gggctgcggc
     5341 cagttgccgg gcggtctcca gacgggtgtc gctacgctcc ccttgctcca gcttccggat
     5401 cagcgagagg gacaccccgg agctcttggc gagctcttgc tgactcatgc cccgacgctt
     5461 gcgtacgtcg cggagctgct taccaatgtc ggcactcatg agtcaagact acggcgcgta
     5521 agctggtgag cacatggaaa agcccccgct tcctggcggg ggcgagggtc cgaggcatgt
     5581 tccccaaagc gataccactt gaagcagtgg tactgcttgt gggtacactc tgcgggtgat
     5641 gaatcgaggg gggcccactg tacgggccga catctacgtc cgaatcagcc tggaccgcac
     5701 aggggaagag ctcggggtcg agcgccagga ggagtcgtgt cgcgagctct gcaagagcct
     5761 cggcatggag gtggggcagg tgtgggtcga caacgacctg agcgccacca agaagaacgt
     5821 cgtccgccct gacttcgagg cgatgatcgc gagcaacccg caggcgatcg tctgctggca
     5881 caccgaccgg ctcatccgcg tcacgcggga cctggagcgg gtgatcgacc tcggagtcaa
     5941 cgtccacgcc gtgatggccg gacacctgga cctgtccacc ccggccggcc gagccgtcgc
     6001 ccgcacggtg acggcctggg ccacgtacga gggcgagcag aaggctgagc gccagaagct
     6061 cgccaacatc cagaacgccc gcgccggcaa gccgtacacc cccggcatcc gccccttcgg
     6121 gtacggcgac gaccacatga ccatcgtgac ggccgaggcg gacgccatcc gcgacggcgc
     6181 gaagatgatc ctcgacggct ggtccctgtc ggccgtggct cgctactggg aggagctcaa
     6241 gctccagtcg ccccggagta tggccgcagg cggcaagggc tggtctctgc ggggcgtaaa
     6301 gaaggtgctg acctccccgc gctacgtcgg gcggtccagc tacctcgggg aggtcgtggg
     6361 cgatgctcag tggccgccca tcctcgaccc ggacgtctac tacggggtcg tggccatcct
     6421 gaacaacccc gaccgcttca gcgggggccc tcggaccggc cgcacccccg gcacgctgct
     6481 cgcaggcatc gccttgtgcg gtgagtgcgg caagacggtc agtggacgcg gctaccgagg
     6541 tgtcctggtc tacggatgta aggacacgca cactcggacg cctcggagca tcgctgacgg
     6601 ccgcgcgagc agctcgaccc tcgcccggct catgttcccc gacttcctgc ccggcctcct
     6661 ggcctctggg caggccgagg acggccagtc ggcagcatcc aagcactcgg aggcccagac
     6721 gctgcgcgag cgccttgacg ggctggctac ggcctacgcg gagggtgcga tcagcctgtc
     6781 tcagatgacg gccggctcgg aagcactgcg gaagaagctg gaggtgatcg aagccgacct
     6841 cgtgggctcg gcaggcatcc cgcccttcga tccagtggcc ggagtggctg gcctgatctc
     6901 cggctggccc accacgcctc tcccgacgcg tcgagcatgg gtggacttct gcctggtggt
     6961 cacgctgaac acccagaagg ggcgccatgc gtcgagcatg accgtggacg accacgtcac
     7021 catcgagtgg cgagacgtgg ccgagtagca gatacgacga agccccggct acccccttct
     7081 gagggtgccg gggctttcgt gtgtcagttg tggtggccgc tctccaggcg gtcgatctct
     7141 tcgagggacg cctcgtagtt gccggcctct tggaggagga gcaggcggag gtcggcgagc
     7201 ttgacctgcg cccacacggg gaatgcatat gcggccgccg caattgattt aaattgaagc
     7261 cgccgaagcc ccgaagcttc cccttgacca atactagtag ttccttcgtc accatagcgg
     7321 gcagggagc
//
