LOCUS       62056_13705        5755 bp DNA     circular SYN 12-MAY-2019
DEFINITION  Cloning vector pLB1, complete sequence.
ACCESSION   MK159854
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..5755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     433..449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(499..595)
                     /regulatory_class="promoter"
     primer_bind     complement(616..632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(640..656)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(664..694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(709..730)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1018..1606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1859..2659
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(3096..3464)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(3497..3606)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_recomb     3795..3842
     CDS             3899..5683
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO31437.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
ORIGIN
        1 gtttaaacgc atgcatatgc ggccgccgca attgatttaa attgaagccg ccgaagcccc
       61 gaagcttccc cttgaccaat actagtgaat ctcgtgcttt cagcttcgat gtaggagggc
      121 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg
      181 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta
      241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
      301 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      361 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      421 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag
      481 gatccttaat taaggcatat ggacactcct tacttagact gtcgtattct cctggccacg
      541 actttacaac accgcacagc atgttgtcaa agcagagacg gttcgaatgt gaacagatat
      601 cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1621 ctactagtag ctccatcagc aaaaggggat gataagttta tcaccaattt aaatctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttggttc atgtgcagct ccatcagcaa aaggggatga
     1801 taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg atcgactgat
     1861 gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg
     1921 tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca gctccttccg
     1981 tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc gtgctgcgct
     2041 gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg agccgttcga
     2101 tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat tctggcgcct
     2161 gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc cacaggcaga
     2221 gcagatcatc tctgatccat tgcccctgcc acctcactcg cctgcaagcc cggtcgcccg
     2281 tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg ccaacacgac
     2341 gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac actgcaccat
     2401 tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact gctgtgagcg
     2461 ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag gtccagtcgg
     2521 tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc tgggtcaact
     2581 gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc
     2641 cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt ggaggggcaa
     2701 ggtcgcgctg attgctgggg caacacgtgg agcggatcgg ggattgtctt tcttcagctc
     2761 gctgatgata tgctgacgct caatgccgtt tggcctccga ctaacgaaaa tcccgcattt
     2821 ggacggctga tccgattggc acggcggacg gcgaatggcg gagcagacgc tcgtccgggg
     2881 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtat cgggccctgg ccagctagct
     2941 agagtcgacc tgcaggtccc cggggatcgg tcttgccttg ctcgtcggtg atgtacttca
     3001 ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc
     3061 gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg gcggaccacg
     3121 cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg
     3181 atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg
     3241 ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg ctcatagtcc
     3301 acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg
     3361 ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg
     3421 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct
     3481 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     3541 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     3601 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     3661 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     3721 cagggttatg cagcggaaaa gatccgtcga cctgcaggca tgctggcgcc ggacggggct
     3781 tcagacgttt cgggtgctgg gttgttgtct ctggacagtg atccatggga aactactcag
     3841 caccaccaat gttcccaaaa gaaagcgcag gtcagcgccc atgagccaag atctaggcat
     3901 gtcgcccttc atcgctcccg acgtccctga gcaccttcta gacactgttc gcgtcttcct
     3961 gtacgcgcgt cagtctaagg gccggtccga cggctcagac gtgtcgaccg aagcacagct
     4021 agcggccggt cgtgcgttgg tcgcgtctcg caacgcccag gggggtgcgc gctgggtcgt
     4081 ggcaggtgag ttcgtggacg tcgggcgctc cggctgggac ccgaacgtga cccgtgccga
     4141 cttcgagcgc atgatgggcg aagtccgcgc cggcgaaggt gacgttgtcg ttgtgaatga
     4201 gctttcccgg ctcactcgca agggcgccca tgacgcgctc gaaatcgaca acgaattgaa
     4261 gaagcacggc gtgcgcttca tgtcggttct tgagccgttc cttgacacgt ctacccctat
     4321 cggcgtcgcc attttcgcgc tgatcgctgc ccttgcgaaa caggacagtg acctgaaggc
     4381 ggagcgcctg aagggtgcga aagacgagat tgccgcgctg ggtggcgttc actcgtcttc
     4441 cgccccgttc ggaatgcgcg ccgtgcgcaa gaaggtcgat aatctcgtga tctccgttct
     4501 tgagccggac gaagacaacc cggatcacgt cgagctagtt gagcgcatgg cgaaaatgtc
     4561 gttcgaaggc gtgtccgaca acgccattgc aacgaccttc gagaaggaaa agatcccgtc
     4621 gcccggaatg gctgagagac gcgccacgga aaagcgtctt gcgtccatca aggcacgtcg
     4681 cctgaacggc gctgaaaagc cgatcatgtg gcgcgctcaa acggtccgat ggattctcaa
     4741 ccatcccgca atcggcggtt tcgcattcga gcgtgtgaag cacggtaagg cgcacatcaa
     4801 cgtcatacgg cgcgaccccg gcggcaagcc gctaacgccc cacacgggca ttctcagcgg
     4861 ctcgaagtgg cttgagcttc aagagaagcg ttccgggaag aatctcagcg accggaagcc
     4921 tggggccgaa gtcgaaccga cgcttctgag cgggtggcgt ttcctggggt gccgaatctg
     4981 cggcggctca atgggtcagt cccagggtgg ccgtaagcgc aacggcgacc ttgccgaagg
     5041 caattacatg tgcgccaacc cgaaggggca cggcggcttg tcggtcaagc gcagcgaact
     5101 ggacgaattc gttgcttcga aggtgtgggc acggctccgc acagccgaca tggaagatga
     5161 acacgatcag gcatggattg ccgccgctgc ggagcgcttc gcccttcagc acgacctagc
     5221 gggggtggcc gatgagcggc gcgaacaaca ggcgcaccta gacaacgtgc ggcgctccat
     5281 caaggacctt caggcggacc gtaaggccgg tctgtacgtc gggcgtgaag agctggaaac
     5341 gtggcgctca acggtgctgc aataccggtc ctacgaagcg gagtgcacga cccgactcgc
     5401 tgagcttgac gagaagatga acggcagcac ccgcgttccg tctgagtggt tcagcggcga
     5461 agacccgacg gccgaagggg gcatctgggc aagctgggac gtgtacgagc gtcgggagtt
     5521 cctgagcttc ttccttgact ccgtcatggt cgaccggggg cgccaccctg agacgaagaa
     5581 atacatcccc ctgaaggacc gtgtgacgct caagtgggcg gagctgctga aggaggaaga
     5641 cgaagcgagc gaagccactg agcgggagct tgcggcgctg tagcgcacag cgggaggggt
     5701 cgagccggcg gacggttcgg cccctttttt ggccttgaaa tcgttagtta ggcta
//
