LOCUS       62056_13700        9496 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pLASW, complete sequence.
ACCESSION   MK424954
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 9496)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..9496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4843..5273
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(5284..5418)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5690..5710)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(5840..5905)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(5983..6037)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(6042..6280)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    complement(6304..6459)
                     /label=LexA binding sites
                     /note="LexA binding sites"
     misc_feature    6551..6981
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    7020..7089
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7308..7326)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7347..7363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7371..7387)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7395..7425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7440..7461)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7749..8337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8511..9368)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9369..9473)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagtat aacttcgtat aatgtatgct atacgaagtt attggccacg taataagtgt
     4861 gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata
     4921 ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat
     4981 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg
     5041 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta
     5101 ataaaataca ttgcataccc tcttttaata aagaatattg catacgttga cgaaacaaat
     5161 tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc cataccattt
     5221 agccgatcaa ttgtgctcgg caacagtata tttgtggtgt gccaaccaac aacacgcgta
     5281 ctggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5341 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5401 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5461 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     5521 gatctctagt caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg
     5581 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     5641 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     5701 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     5761 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     5821 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     5881 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     5941 attatgtcac accacagaag taaggttcct tcacaaagat cctctagagg taccctcgag
     6001 ccgcggccgc agatctgtta acgaattccc aattccctat tcagagttct cttcttgtat
     6061 tcaataatta cttcttggca gatttcagta gttgcagttg atttacttgg ttgctggtta
     6121 cttttaattg attcacttta acttgcactt tactgcagat tgtttagctt gttcagctgc
     6181 gcttgtttat ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa
     6241 ttgtcgctcc gtagacgaag cgcctctatt tatactccgg cgctcaagct tatcatccct
     6301 cgacgtactg tacatataac cactggtttt atatacagca gtactgtaca tataaccact
     6361 ggttttatat acagcagtcg acgtactgta catataacca ctggttttat atacagcagt
     6421 actgtacata taaccactgg ttttatatac agcagtcgag gtaagattag gatccggaga
     6481 gctcccaacg cgttggatgc agccaagctt tgcgtactcg caaattatta aaaatggatc
     6541 ctcaaggcct tggccacgta ataagtgtgc gttgaattta ttcgcaaaaa cattgcatat
     6601 tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg catacttttt
     6661 agagaaacca aataattttt tattgcatac ccgtttttaa taaaatacat tgcataccct
     6721 cttttaataa aaaatattgc atactttgac gaaacaaatt ttcgttgcat acccaataaa
     6781 agattattat attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     6841 gaatattgca tacgttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     6901 ttgcatacct tttcttgcca taccatttag ccgatcaatt gtgctcggca acagtatatt
     6961 tgtggtgtgc caaccaacaa cactagagat ccactagtgt cgacgatgta ggtcacggtc
     7021 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     7081 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     7141 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     7201 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     7261 acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct ttagtgaggg
     7321 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7381 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7441 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7501 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7561 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7621 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7681 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7741 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7801 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7861 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7921 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7981 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8041 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8101 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8161 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8221 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8341 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8461 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8521 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8581 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8641 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8701 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8761 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8821 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8881 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8941 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9001 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9061 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9121 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9181 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9241 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9481 ccccgaaaag tgccac
//
