LOCUS       62056_13695        7240 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pLASV, complete sequence.
ACCESSION   MK424964
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 7240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..7240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(3028..3162)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3434..3454)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(3584..3649)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(3727..3781)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(3786..4024)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    complement(4048..4203)
                     /label=LexA binding sites
                     /note="LexA binding sites"
     misc_feature    4295..4725
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    4764..4833
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(5052..5070)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5091..5107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5115..5131)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5139..5169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5184..5205)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5493..6081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6255..7112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7113..7217)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtactggat ccagacatga taagatacat tgatgagttt
     3061 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3121 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3181 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3241 tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt
     3301 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     3361 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     3421 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     3481 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     3541 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     3601 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     3661 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     3721 agatcctcta gaggtaccct cgagccgcgg ccgcagatct gttaacgaat tcccaattcc
     3781 ctattcagag ttctcttctt gtattcaata attacttctt ggcagatttc agtagttgca
     3841 gttgatttac ttggttgctg gttactttta attgattcac tttaacttgc actttactgc
     3901 agattgttta gcttgttcag ctgcgcttgt ttatttgctt agctttcgct tagcgacgtg
     3961 ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc tatttatact
     4021 ccggcgctca agcttatcat ccctcgacgt actgtacata taaccactgg ttttatatac
     4081 agcagtactg tacatataac cactggtttt atatacagca gtcgacgtac tgtacatata
     4141 accactggtt ttatatacag cagtactgta catataacca ctggttttat atacagcagt
     4201 cgaggtaaga ttaggatccg gagagctccc aacgcgttgg atgcagccaa gctttgcgta
     4261 ctcgcaaatt attaaaaatg gatcctcaag gccttggcca cgtaataagt gtgcgttgaa
     4321 tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca taccttatca
     4381 aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc atacccgttt
     4441 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt tgacgaaaca
     4501 aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt taataaaata
     4561 cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa attttcgttg
     4621 catacccaat aaaagattat tatattgcat accttttctt gccataccat ttagccgatc
     4681 aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacactag agatccacta
     4741 gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg
     4801 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg
     4861 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg
     4921 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg
     4981 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacact agttctagcc
     5041 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
     5101 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5161 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5221 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5281 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5341 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7201 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
