LOCUS       62056_13635        3282 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(715..733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(743..759)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      901..1356
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1382..1486
                     /label=AmpR promoter
     CDS             1487..2344
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2518..3106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctagctatca
       61 acagatttgc aattggttca cacaaaaacg ctcgagttcc cgtaccttag ctccagtaga
      121 cctagtgcag gtctcgtcag ctcagacagc ggcgcagctg ctcccgggat tccaactcaa
      181 cttgctccag tccctgtttg gagaacaacg tcaaaaattt gatttttacg acaaactgga
      241 tgaaaccaag attgtcggcg gctccgactc gccatctcca gccgatgatg agattcatag
      301 caattcagat gaaactattc aagatacttt gttttccatc aatattaaac cagaaccgga
      361 aattgattca attgcatcct ccccggatat ggcaaactca atgcgcgaat cattgtcctc
      421 gacctcccca aagcttctca gcgggctcga cctcggagca ttcagcaccc actcatcatc
      481 caccaactcc atgcaaaacg ccaactcatc agcggctcgc tcacgcctga tgttcgaccc
      541 actcaccgag ctacccgtcc tcgaaaaatg gttcgaagag aatcctcacc cgacatggat
      601 gcaaatcgat cagtacacac aatgcttgaa caactgcgct tatcgagaga attatccgca
      661 tattagtcag cacaatgtga agatctggtt caaaaatcga ggtacccaat tcgccctata
      721 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      781 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      841 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
      901 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      961 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1021 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1081 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1141 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1201 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1261 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1321 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     1381 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1441 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1501 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1561 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1621 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1681 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1741 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1801 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1861 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1921 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1981 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2041 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2101 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2161 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2221 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2281 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2341 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2401 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2461 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2581 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2641 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2821 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3181 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3241 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gc
//
