LOCUS       40924_27509        3699 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pL2AD-lc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 3699)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(60..648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(822..1679)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    complement(1777..2280)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2541..2809
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        2818..2892
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      2897..2916
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      2968..3157
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3376..3393
                     /note="I-SceI; cleavage site"
     misc_feature    3394..3443
                     /label=homology region A0*
                     /note="homology region A0*"
     misc_feature    3450..3499
                     /label=homology region E0*
                     /note="homology region E0*"
     misc_feature    3500..3517
                     /note="I-SceI; cleavage site"
ORIGIN
        1 atgtgagcaa aaggccagca aaagcccagg aaccgtaaaa aggccgcgtt gctggcgttt
       61 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      481 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
      541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      661 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      721 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      781 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      841 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      901 tagataacta cgatacggga gcgcttacca tctggcccca gtgctgcaat gataccgcga
      961 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1021 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1081 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttggcat tgctacaggc
     1141 atcgtggtgt cactctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1201 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1261 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1321 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1381 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1441 gataatagtg tatcacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1501 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     1561 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1621 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1681 ctcttccttt ttcaatgggt aataactgat ataattaaat tgaagctcta atttgtgagt
     1741 ttagtataca tgcatttact tataatacag ttgacggatc gcttgcctgt aacttacacg
     1801 cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt
     1861 atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag
     1921 aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt
     1981 tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta
     2041 aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta
     2101 atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt
     2161 cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat
     2221 ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat
     2281 gaaaaggacc acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2341 ttttttaacg aatagcccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2401 atagggttga gtgttgttcc agtttccaac aagagtccac tattaaagaa cgtggactcc
     2461 aacgtcaaag ggcgaaaaag ggtctatcag ggcgatggcc cactacgtga accatcaccc
     2521 taatcaagtt ttttggggtc tctttgaaaa gataatgtat gattatgctt tcactcatat
     2581 ttatacagaa acttgatgtt ttctttcgag tatatacaag gtgattacat gtacgtttga
     2641 agtacaactc tagattttgt agtgccctct tgggctagcg gtaaaggtgc gcattttttc
     2701 acaccctaca atgttctgtt caaaagattt tggtcaaacg ctgtagaagt gaaagttggt
     2761 gcgcatgttt cggcgttcga aacttctccg cagtgaaaga taaatgatcg cggccgcgtt
     2821 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2881 accgagtcgg tggtgctttt tttgtttttt atgtcttcga gtcatgtaat tagttatgtc
     2941 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     3001 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     3061 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     3121 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaggtgccg taaagcagta
     3181 aatcggaagg gtaaacggat gcccccattt agagcttgac ggggaaagcc ggcgaacgtg
     3241 gcgagaaagg aagggaagaa agcgaaagga gcgggggcta gggcggtggg aagtgtaggg
     3301 gtcacgctgg gcgtaaccac cacacccgcc gcgcttaatg gggcgctaca gggcgcgtgg
     3361 ggatgatcca ctagttaggg ataacagggt aataaatagg aaagtctggt aactcaggac
     3421 actgtatctg ctacgctgtt tatgaattct ctcttacaat aaaatagaat actctgcgaa
     3481 gggcgtgaca ctgctgttta ttaccctgtt atccctacgg ccctgcatta atgaatcggc
     3541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3661 cggttatcca cagaatcagg ggataacgca ggaaagaac
//
