LOCUS       40924_27489        3699 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pL2AB-lc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 3699)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(58..646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(820..1677)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    complement(1775..2278)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2539..2807
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        2816..2890
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      2895..2914
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      2966..3155
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3374..3391
                     /note="I-SceI; cleavage site"
     misc_feature    3392..3441
                     /label=homology region A0*
                     /note="homology region A0*"
     misc_feature    3448..3497
                     /label=homology region C0*
                     /note="homology region C0*"
     misc_feature    3498..3515
                     /note="I-SceI; cleavage site"
ORIGIN
        1 gtgagcaaaa ggccagcaaa agcccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
       61 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      481 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
      721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
      901 gataactacg atacgggagc gcttaccatc tggccccagt gctgcaatga taccgcgaga
      961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttggcattg ctacaggcat
     1141 cgtggtgtca ctctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1441 taatagtgta tcacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     1681 cttccttttt caatgggtaa taactgatat aattaaattg aagctctaat ttgtgagttt
     1741 agtatacatg catttactta taatacagtt gacggatcgc ttgcctgtaa cttacacgcg
     1801 cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat
     1861 gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa
     1921 aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta
     1981 ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa
     2041 atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat
     2101 acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct
     2161 tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt
     2221 ttaatttata tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga
     2281 aaaggaccac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2341 ttttaacgaa tagcccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2401 agggttgagt gttgttccag tttccaacaa gagtccacta ttaaagaacg tggactccaa
     2461 cgtcaaaggg cgaaaaaggg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2521 atcaagtttt ttggggtctc tttgaaaaga taatgtatga ttatgctttc actcatattt
     2581 atacagaaac ttgatgtttt ctttcgagta tatacaaggt gattacatgt acgtttgaag
     2641 tacaactcta gattttgtag tgccctcttg ggctagcggt aaaggtgcgc attttttcac
     2701 accctacaat gttctgttca aaagattttg gtcaaacgct gtagaagtga aagttggtgc
     2761 gcatgtttcg gcgttcgaaa cttctccgca gtgaaagata aatgatcgcg gccgcgtttt
     2821 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     2881 cgagtcggtg gtgctttttt tgttttttat gtcttcgagt catgtaatta gttatgtcac
     2941 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
     3001 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
     3061 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga
     3121 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aggtgccgta aagcagtaaa
     3181 tcggaagggt aaacggatgc ccccatttag agcttgacgg ggaaagccgg cgaacgtggc
     3241 gagaaaggaa gggaagaaag cgaaaggagc gggggctagg gcggtgggaa gtgtaggggt
     3301 cacgctgggc gtaaccacca cacccgccgc gcttaatggg gcgctacagg gcgcgtgggg
     3361 atgatccact agttagggat aacagggtaa taaataggaa agtctggtaa ctcaggacac
     3421 tgtatctgct acgctgttta tgaattctat tgcgtgtgat acttcaggag aaccgttatt
     3481 ccgtaaaact acagtcaatt accctgttat ccctacggcc ctgcattaat gaatcggcca
     3541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3661 gttatccaca gaatcagggg ataacgcagg aaagaacat
//
