LOCUS       40924_27484        4653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pL2AB-hc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 4653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(58..646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(820..1677)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(2361..3241)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3493..3761
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
     misc_RNA        3770..3844
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      3849..3868
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4
                     tRNA gene"
     terminator      3920..4109
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    4328..4345
                     /note="I-SceI; cleavage site"
     misc_feature    4346..4395
                     /label=homology region A0*
                     /note="homology region A0*"
     misc_feature    4402..4451
                     /label=homology region C0*
                     /note="homology region C0*"
     misc_feature    4452..4469
                     /note="I-SceI; cleavage site"
ORIGIN
        1 gtgagcaaaa ggccagcaaa agcccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
       61 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      481 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
      721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
      901 gataactacg atacgggagc gcttaccatc tggccccagt gctgcaatga taccgcgaga
      961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttggcattg ctacaggcat
     1141 cgtggtgtca ctctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1441 taatagtgta tcacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     1681 cttccttttt caatgggtaa taactgatat aattaaattg aagctctaat ttgtgagttt
     1741 agtatacatg catttactta taatacagtt ttatcgatga taagctgtca aagatgagaa
     1801 ttaattccac ggactataga ctatactaga tactccgtct actgtacgat acacttccgc
     1861 tcaggtcctt gtcctttaac gaggccttac cactcttttg ttactctatt gatccagctc
     1921 agcaaaggca gtgtgatcta agattctatc ttcgcgatgt agtaaaacta gctagaccga
     1981 gaaagagact agaaatgcaa aaggcacttc tacaatggct gccatcatta ttatccgatg
     2041 tgacgctgca gcttctcaat gatattcgaa tacgctttga ggagatacag cctaatatcc
     2101 gacaaactgt tttacagatt tacgatcgta cttgttaccc atcattgaat tttgaacatc
     2161 cgaacctggg agttttccct gaaacagata gtatatttga acctgtataa taatatatag
     2221 tctagcgctt tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta
     2281 ttgcataggt aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac
     2341 ttcaatagca tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     2401 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     2461 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     2521 atgcaacgcg acgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     2581 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
     2641 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
     2701 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
     2761 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
     2821 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
     2881 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
     2941 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
     3001 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
     3061 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
     3121 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
     3181 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
     3241 tgacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3301 cgaatagccc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3361 gagtgttgtt ccagtttcca acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3421 agggcgaaaa agggtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3481 ttttttgggg tctctttgaa aagataatgt atgattatgc tttcactcat atttatacag
     3541 aaacttgatg ttttctttcg agtatataca aggtgattac atgtacgttt gaagtacaac
     3601 tctagatttt gtagtgccct cttgggctag cggtaaaggt gcgcattttt tcacacccta
     3661 caatgttctg ttcaaaagat tttggtcaaa cgctgtagaa gtgaaagttg gtgcgcatgt
     3721 ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgcggccgcg ttttagagct
     3781 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3841 ggtggtgctt tttttgtttt ttatgtcttc gagtcatgta attagttatg tcacgcttac
     3901 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     3961 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     4021 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     4081 tgcttgagaa ggttttggga cgctcgaagg ctttaggtgc cgtaaagcag taaatcggaa
     4141 gggtaaacgg atgcccccat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4201 ggaagggaag aaagcgaaag gagcgggggc tagggcggtg ggaagtgtag gggtcacgct
     4261 gggcgtaacc accacacccg ccgcgcttaa tggggcgcta cagggcgcgt ggggatgatc
     4321 cactagttag ggataacagg gtaataaata ggaaagtctg gtaactcagg acactgtatc
     4381 tgctacgctg tttatgaatt ctattgcgtg tgatacttca ggagaaccgt tattccgtaa
     4441 aactacagtc aattaccctg ttatccctac ggccctgcat taatgaatcg gccaacgcgc
     4501 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4621 cacagaatca ggggataacg caggaaagaa cat
//
